Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.

The main goal of this study was to evaluate if the administration of cannabidiol (CBD) regulates behavioral and gene expression alterations induced by spontaneous alcohol withdrawal (SAW) in mice. Increasing doses of ethanol were administered to C57BL/6J male mice for 15 days (2.5, 3 and 3.5 g/kg/12...

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Autores: Gasparyan A, Navarrete F, Navarro D, Manzanares J
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
Repositorio:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
OAI Identifier:oai:isabial.fundanetsuite.com:p9578
Acceso en línea:https://isabial.portalinvestigacion.com/publicaciones9578
https://www.sciencedirect.com/science/article/pii/S0028390823001399?via%3Dihub
Access Level:acceso abierto
Palabra clave:Alcohol withdrawal
Cannabidiol
Correlation studies
Gene expression
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spelling Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.Gasparyan ANavarrete FNavarro DManzanares JAlcohol withdrawalCannabidiolCorrelation studiesGene expressionThe main goal of this study was to evaluate if the administration of cannabidiol (CBD) regulates behavioral and gene expression alterations induced by spontaneous alcohol withdrawal (SAW) in mice. Increasing doses of ethanol were administered to C57BL/6J male mice for 15 days (2.5, 3 and 3.5 g/kg/12 h, p. o.), and SAW was studied at 6, 12, 24, and 72 h after the last ethanol administration. The efficacy of acute CBD (10, 20, and 40 mg/kg, i. p.) to regulate behavioral changes induced by SAW was explored at 6 h. Gene expression analyses of cannabinoid receptors 1 (Cnr1) and 2 (Cnr2), mu-opioid receptor (Opmr1), and proopiomelanocortin (Pomc) in the nucleus accumbens (NAcc), and Pomc and tyrosine hydroxylase (Th) in the ventral tegmental area (VTA), were carried out by real time-PCR. Pearson correlation was used to identify potential associations between the gene expression data and the anxiety-like behaviors. Biostatistical studies suggest associations between gene expression data and the anxiogenic behaviors in mice exposed to the SAW model and treated with VEH and 40 mg/kg of CBD. Mice exposed to the SAW model showed significant somatic withdrawal signs, anxiety-like behaviors, and remarkable changes in the gene expression of all brain targets at 6 h. CBD dose-dependently normalized the behavioral, somatic withdrawal signs and anxiety-like behaviors and modulated gene expression changes in the NAcc, but not in the VTA. The results of this study suggest that CBD may regulate specific alcohol withdrawal-associated alterations. However, further studies are required to explore the possible mechanisms involved.PERGAMON-ELSEVIER SCIENCE LTD2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://isabial.portalinvestigacion.com/publicaciones9578https://www.sciencedirect.com/science/article/pii/S0028390823001399?via%3DihubNEUROPHARMACOLOGYISSN: 00283908ISSNe: 18737064reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicanteinstname:Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)Inglésinfo:eu-repo/semantics/openAccessoai:isabial.fundanetsuite.com:p95782026-06-12T10:20:37Z
dc.title.none.fl_str_mv Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.
title Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.
spellingShingle Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.
Gasparyan A
Alcohol withdrawal
Cannabidiol
Correlation studies
Gene expression
title_short Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.
title_full Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.
title_fullStr Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.
title_full_unstemmed Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.
title_sort Cannabidiol regulates behavioral and brain alterations induced by spontaneous alcohol withdrawal.
dc.creator.none.fl_str_mv Gasparyan A
Navarrete F
Navarro D
Manzanares J
author Gasparyan A
author_facet Gasparyan A
Navarrete F
Navarro D
Manzanares J
author_role author
author2 Navarrete F
Navarro D
Manzanares J
author2_role author
author
author
dc.subject.none.fl_str_mv Alcohol withdrawal
Cannabidiol
Correlation studies
Gene expression
topic Alcohol withdrawal
Cannabidiol
Correlation studies
Gene expression
description The main goal of this study was to evaluate if the administration of cannabidiol (CBD) regulates behavioral and gene expression alterations induced by spontaneous alcohol withdrawal (SAW) in mice. Increasing doses of ethanol were administered to C57BL/6J male mice for 15 days (2.5, 3 and 3.5 g/kg/12 h, p. o.), and SAW was studied at 6, 12, 24, and 72 h after the last ethanol administration. The efficacy of acute CBD (10, 20, and 40 mg/kg, i. p.) to regulate behavioral changes induced by SAW was explored at 6 h. Gene expression analyses of cannabinoid receptors 1 (Cnr1) and 2 (Cnr2), mu-opioid receptor (Opmr1), and proopiomelanocortin (Pomc) in the nucleus accumbens (NAcc), and Pomc and tyrosine hydroxylase (Th) in the ventral tegmental area (VTA), were carried out by real time-PCR. Pearson correlation was used to identify potential associations between the gene expression data and the anxiety-like behaviors. Biostatistical studies suggest associations between gene expression data and the anxiogenic behaviors in mice exposed to the SAW model and treated with VEH and 40 mg/kg of CBD. Mice exposed to the SAW model showed significant somatic withdrawal signs, anxiety-like behaviors, and remarkable changes in the gene expression of all brain targets at 6 h. CBD dose-dependently normalized the behavioral, somatic withdrawal signs and anxiety-like behaviors and modulated gene expression changes in the NAcc, but not in the VTA. The results of this study suggest that CBD may regulate specific alcohol withdrawal-associated alterations. However, further studies are required to explore the possible mechanisms involved.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://isabial.portalinvestigacion.com/publicaciones9578
https://www.sciencedirect.com/science/article/pii/S0028390823001399?via%3Dihub
url https://isabial.portalinvestigacion.com/publicaciones9578
https://www.sciencedirect.com/science/article/pii/S0028390823001399?via%3Dihub
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv PERGAMON-ELSEVIER SCIENCE LTD
publisher.none.fl_str_mv PERGAMON-ELSEVIER SCIENCE LTD
dc.source.none.fl_str_mv NEUROPHARMACOLOGY
ISSN: 00283908
ISSNe: 18737064
reponame:r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
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instname_str Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL)
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