Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration [Dataset]

Retinal neurodegenerative diseases are the leading causes of visual impairment and irreversible blindness worldwide. Although the retinal response to injury remains closely similar between different retinal neurodegenerative diseases, available therapeutic alternatives are only palliative, too expen...

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Autores: Puertas-Neyra, Kevin, Galindo-Cabello, Nadia, Hernández-Rodríguez, Leticia A., González-Pérez, Fernando, Rodríguez-Cabello, José Carlos, González-Sarmiento, Rogelio, Pastor, José Carlos, Usategui-Martín, Ricardo, Fernández-Bueno, Iván
Tipo de recurso: conjunto de datos
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/329975
Acceso en línea:http://hdl.handle.net/10261/329975
Access Level:acceso abierto
Palabra clave:Retina
Neurodegeneration
Autophagy
Apoptosis
Necroptosis
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spelling Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration [Dataset]Puertas-Neyra, KevinGalindo-Cabello, NadiaHernández-Rodríguez, Leticia A.González-Pérez, FernandoRodríguez-Cabello, José CarlosGonzález-Sarmiento, RogelioPastor, José CarlosUsategui-Martín, RicardoFernández-Bueno, IvánRetinaNeurodegenerationAutophagyApoptosisNecroptosisRetinal neurodegenerative diseases are the leading causes of visual impairment and irreversible blindness worldwide. Although the retinal response to injury remains closely similar between different retinal neurodegenerative diseases, available therapeutic alternatives are only palliative, too expensive, or very specific, such as gene therapy. In that sense, the development of broad-spectrum neuroprotective therapies seems to be an excellent option. In this regard, it is essential to identify molecular targets involved in retinal degeneration, such as cell death mechanisms. Apoptosis has been considered as the primary cell death mechanism during retinal degeneration; however, recent studies have demonstrated that the only use of anti-apoptotic drugs is not enough to confer good neuroprotection in terms of cell viability and preservation. For that reason, the interrelationship that exists between apoptosis and other cell death mechanisms needs to be characterized deeply to design future therapeutic options that simultaneously block the main cell death pathways. In that sense, the study aimed to characterize the programmed cell death (in terms of apoptosis and necroptosis) and autophagy response and modulation in retinal neurodegenerative diseases, using an in vitro model of spontaneous retinal neurodegeneration. For that purpose, we measured the mRNA relative expression through qPCR of a selected pool of genes involved in apoptosis (BAX, BCL2, CASP3, CASP8, and CASP9), necroptosis (MLKL, RIPK1, and RIPK3), and autophagy (ATG7, BCLIN1, LC3B, mTOR, and SQSTM1); besides, the immunoexpression of their encoding proteins (Casp3, MLKL, RIPK1, LC3B, and p62) were analyzed using immunohistochemistry. Our results showed an increase of pro-apoptotic and pro-necroptotic related genes and proteins during in vitro retinal neurodegeneration. Besides, we describe for the first time the modulation between programmed cell death mechanisms and autophagy in an in vitro retinal neurodegeneration model. This study reinforces the idea that cell death mechanisms are closely interconnected and provides new information about molecular signaling and autophagy along the retinal degeneration process.Peer reviewedFigshareConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1Publisher's versioninfo:eu-repo/semantics/publishedVersionimage/jpeghttp://hdl.handle.net/10261/329975reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésPuertas-Neyra, Kevin; Galindo-Cabello, Nadia; Hernández-Rodríguez, Leticia A.; González-Pérez, Fernando; Rodríguez-Cabello, José Carlos; González-Sarmiento, Rogelio; Pastor, José Carlos; Usategui-Martín, Ricardo; Fernández-Bueno, Iván; 2022; Image_1_Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration.JPEG [Dataset]; Figshare; https://doi.org/10.3389/fnana.2022.812487.s001Puertas-Neyra, Kevin; Galindo-Cabello, Nadia; Hernández-Rodríguez, Leticia A.; González-Pérez, Fernando; Rodríguez-Cabello, José Carlos; González-Sarmiento, Rogelio; Pastor, José Carlos; Usategui-Martín, Ricardo; Fernández-Bueno, Iván. Programmed cell death and autophagy in an in vitro model of spontaneous neuroretinal degeneration. http://dx.doi.org/10.3389/fnana.2022.812487. http://hdl.handle.net/10261/283072https://doi.org/10.3389/fnana.2022.812487.s001Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3299752026-05-22T06:33:51Z
dc.title.none.fl_str_mv Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration [Dataset]
title Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration [Dataset]
spellingShingle Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration [Dataset]
Puertas-Neyra, Kevin
Retina
Neurodegeneration
Autophagy
Apoptosis
Necroptosis
title_short Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration [Dataset]
title_full Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration [Dataset]
title_fullStr Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration [Dataset]
title_full_unstemmed Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration [Dataset]
title_sort Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration [Dataset]
dc.creator.none.fl_str_mv Puertas-Neyra, Kevin
Galindo-Cabello, Nadia
Hernández-Rodríguez, Leticia A.
González-Pérez, Fernando
Rodríguez-Cabello, José Carlos
González-Sarmiento, Rogelio
Pastor, José Carlos
Usategui-Martín, Ricardo
Fernández-Bueno, Iván
author Puertas-Neyra, Kevin
author_facet Puertas-Neyra, Kevin
Galindo-Cabello, Nadia
Hernández-Rodríguez, Leticia A.
González-Pérez, Fernando
Rodríguez-Cabello, José Carlos
González-Sarmiento, Rogelio
Pastor, José Carlos
Usategui-Martín, Ricardo
Fernández-Bueno, Iván
author_role author
author2 Galindo-Cabello, Nadia
Hernández-Rodríguez, Leticia A.
González-Pérez, Fernando
Rodríguez-Cabello, José Carlos
González-Sarmiento, Rogelio
Pastor, José Carlos
Usategui-Martín, Ricardo
Fernández-Bueno, Iván
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Retina
Neurodegeneration
Autophagy
Apoptosis
Necroptosis
topic Retina
Neurodegeneration
Autophagy
Apoptosis
Necroptosis
description Retinal neurodegenerative diseases are the leading causes of visual impairment and irreversible blindness worldwide. Although the retinal response to injury remains closely similar between different retinal neurodegenerative diseases, available therapeutic alternatives are only palliative, too expensive, or very specific, such as gene therapy. In that sense, the development of broad-spectrum neuroprotective therapies seems to be an excellent option. In this regard, it is essential to identify molecular targets involved in retinal degeneration, such as cell death mechanisms. Apoptosis has been considered as the primary cell death mechanism during retinal degeneration; however, recent studies have demonstrated that the only use of anti-apoptotic drugs is not enough to confer good neuroprotection in terms of cell viability and preservation. For that reason, the interrelationship that exists between apoptosis and other cell death mechanisms needs to be characterized deeply to design future therapeutic options that simultaneously block the main cell death pathways. In that sense, the study aimed to characterize the programmed cell death (in terms of apoptosis and necroptosis) and autophagy response and modulation in retinal neurodegenerative diseases, using an in vitro model of spontaneous retinal neurodegeneration. For that purpose, we measured the mRNA relative expression through qPCR of a selected pool of genes involved in apoptosis (BAX, BCL2, CASP3, CASP8, and CASP9), necroptosis (MLKL, RIPK1, and RIPK3), and autophagy (ATG7, BCLIN1, LC3B, mTOR, and SQSTM1); besides, the immunoexpression of their encoding proteins (Casp3, MLKL, RIPK1, LC3B, and p62) were analyzed using immunohistochemistry. Our results showed an increase of pro-apoptotic and pro-necroptotic related genes and proteins during in vitro retinal neurodegeneration. Besides, we describe for the first time the modulation between programmed cell death mechanisms and autophagy in an in vitro retinal neurodegeneration model. This study reinforces the idea that cell death mechanisms are closely interconnected and provides new information about molecular signaling and autophagy along the retinal degeneration process.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
Publisher's version
info:eu-repo/semantics/publishedVersion
format dataset
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/329975
url http://hdl.handle.net/10261/329975
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Puertas-Neyra, Kevin; Galindo-Cabello, Nadia; Hernández-Rodríguez, Leticia A.; González-Pérez, Fernando; Rodríguez-Cabello, José Carlos; González-Sarmiento, Rogelio; Pastor, José Carlos; Usategui-Martín, Ricardo; Fernández-Bueno, Iván; 2022; Image_1_Programmed Cell Death and Autophagy in an in vitro Model of Spontaneous Neuroretinal Degeneration.JPEG [Dataset]; Figshare; https://doi.org/10.3389/fnana.2022.812487.s001
Puertas-Neyra, Kevin; Galindo-Cabello, Nadia; Hernández-Rodríguez, Leticia A.; González-Pérez, Fernando; Rodríguez-Cabello, José Carlos; González-Sarmiento, Rogelio; Pastor, José Carlos; Usategui-Martín, Ricardo; Fernández-Bueno, Iván. Programmed cell death and autophagy in an in vitro model of spontaneous neuroretinal degeneration. http://dx.doi.org/10.3389/fnana.2022.812487. http://hdl.handle.net/10261/283072
https://doi.org/10.3389/fnana.2022.812487.s001
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv image/jpeg
dc.publisher.none.fl_str_mv Figshare
publisher.none.fl_str_mv Figshare
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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