Satellite cells and markers of muscle regeneration during unloading and reloading: effects of treatment with resveratrol and curcumin

We hypothesized that treatment with pharmacological agents known to increase sirtuin-1 activity (resveratrol and curcumin) may enhance muscle regeneration. In limb muscles of mice (C57BL/6J, 10 weeks) exposed to reloading for seven days following a seven-day period of hindlimb immobilization with/wi...

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Detalhes bibliográficos
Autores: Mañas García, Laura, 1991-, Guitart, Maria, Duran Jordà, Xavier, 1974-, Barreiro Portela, Esther
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/45520
Acesso em linha:http://hdl.handle.net/10230/45520
http://dx.doi.org/10.3390/nu12061870
Access Level:acceso abierto
Palavra-chave:Activated satellite cells
Muscle progenitor cells
Muscle regeneration markers
Muscle reloading
Muscle unloading
Quiescent satellite cells
Sirtuin-1
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spelling Satellite cells and markers of muscle regeneration during unloading and reloading: effects of treatment with resveratrol and curcuminMañas García, Laura, 1991-Guitart, MariaDuran Jordà, Xavier, 1974-Barreiro Portela, EstherActivated satellite cellsMuscle progenitor cellsMuscle regeneration markersMuscle reloadingMuscle unloadingQuiescent satellite cellsSirtuin-1We hypothesized that treatment with pharmacological agents known to increase sirtuin-1 activity (resveratrol and curcumin) may enhance muscle regeneration. In limb muscles of mice (C57BL/6J, 10 weeks) exposed to reloading for seven days following a seven-day period of hindlimb immobilization with/without curcumin or resveratrol treatment, progenitor muscle cell numbers (FACS), satellite cell subtypes (histology), early and late muscle regeneration markers, phenotype and morphometry, sirtuin-1 activity and content, and muscle function were assessed. Treatment with either resveratrol or curcumin in immobilized muscles elicited a significant improvement in numbers of progenitor, activated, quiescent, and total counts of muscle satellite cells, compared to non-treated animals. Treatment with either resveratrol or curcumin in reloaded muscles compared to non-treated mice induced a significant improvement in the CSA of both hybrid (curcumin) and fast-twitch fibers (resveratrol), sirtuin-1 activity (curcumin), sirtuin-1 content (resveratrol), and counts of progenitor muscle cells (resveratrol). Treatment with the pharmacological agents resveratrol and curcumin enhanced the numbers of satellite cells (muscle progenitor, quiescent, activated, and total satellite cells) in the unloaded limb muscles but not in the reloaded muscles. These findings have potential clinical implications as treatment with these phenolic compounds would predominantly be indicated during disuse muscle atrophy to enhance the muscle regeneration process.MDPI202020202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/45520http://dx.doi.org/10.3390/nu12061870reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésNutrients. 2020 Jun 23;12(6):1870Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/455202026-06-12T07:21:37Z
dc.title.none.fl_str_mv Satellite cells and markers of muscle regeneration during unloading and reloading: effects of treatment with resveratrol and curcumin
title Satellite cells and markers of muscle regeneration during unloading and reloading: effects of treatment with resveratrol and curcumin
spellingShingle Satellite cells and markers of muscle regeneration during unloading and reloading: effects of treatment with resveratrol and curcumin
Mañas García, Laura, 1991-
Activated satellite cells
Muscle progenitor cells
Muscle regeneration markers
Muscle reloading
Muscle unloading
Quiescent satellite cells
Sirtuin-1
title_short Satellite cells and markers of muscle regeneration during unloading and reloading: effects of treatment with resveratrol and curcumin
title_full Satellite cells and markers of muscle regeneration during unloading and reloading: effects of treatment with resveratrol and curcumin
title_fullStr Satellite cells and markers of muscle regeneration during unloading and reloading: effects of treatment with resveratrol and curcumin
title_full_unstemmed Satellite cells and markers of muscle regeneration during unloading and reloading: effects of treatment with resveratrol and curcumin
title_sort Satellite cells and markers of muscle regeneration during unloading and reloading: effects of treatment with resveratrol and curcumin
dc.creator.none.fl_str_mv Mañas García, Laura, 1991-
Guitart, Maria
Duran Jordà, Xavier, 1974-
Barreiro Portela, Esther
author Mañas García, Laura, 1991-
author_facet Mañas García, Laura, 1991-
Guitart, Maria
Duran Jordà, Xavier, 1974-
Barreiro Portela, Esther
author_role author
author2 Guitart, Maria
Duran Jordà, Xavier, 1974-
Barreiro Portela, Esther
author2_role author
author
author
dc.subject.none.fl_str_mv Activated satellite cells
Muscle progenitor cells
Muscle regeneration markers
Muscle reloading
Muscle unloading
Quiescent satellite cells
Sirtuin-1
topic Activated satellite cells
Muscle progenitor cells
Muscle regeneration markers
Muscle reloading
Muscle unloading
Quiescent satellite cells
Sirtuin-1
description We hypothesized that treatment with pharmacological agents known to increase sirtuin-1 activity (resveratrol and curcumin) may enhance muscle regeneration. In limb muscles of mice (C57BL/6J, 10 weeks) exposed to reloading for seven days following a seven-day period of hindlimb immobilization with/without curcumin or resveratrol treatment, progenitor muscle cell numbers (FACS), satellite cell subtypes (histology), early and late muscle regeneration markers, phenotype and morphometry, sirtuin-1 activity and content, and muscle function were assessed. Treatment with either resveratrol or curcumin in immobilized muscles elicited a significant improvement in numbers of progenitor, activated, quiescent, and total counts of muscle satellite cells, compared to non-treated animals. Treatment with either resveratrol or curcumin in reloaded muscles compared to non-treated mice induced a significant improvement in the CSA of both hybrid (curcumin) and fast-twitch fibers (resveratrol), sirtuin-1 activity (curcumin), sirtuin-1 content (resveratrol), and counts of progenitor muscle cells (resveratrol). Treatment with the pharmacological agents resveratrol and curcumin enhanced the numbers of satellite cells (muscle progenitor, quiescent, activated, and total satellite cells) in the unloaded limb muscles but not in the reloaded muscles. These findings have potential clinical implications as treatment with these phenolic compounds would predominantly be indicated during disuse muscle atrophy to enhance the muscle regeneration process.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/45520
http://dx.doi.org/10.3390/nu12061870
url http://hdl.handle.net/10230/45520
http://dx.doi.org/10.3390/nu12061870
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nutrients. 2020 Jun 23;12(6):1870
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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