Glucagon-like peptide-1 (GLP-1) and glucose metabolism in human myocytes.

Glucagon-like peptide-1 (GLP-1) has been shown to have insulin-like effects upon the metabolism of glucose in rat liver, muscle and fat, and on that of lipids in rat and human adipocytes. These actions seem to be exerted through specific receptors which, unlike that of the pancreas, are not - at lea...

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Autores: Luque, M.A., Márquez, L., Acitores, A., Redondo, A., Morales, M., Valverde, I., Villanueva-Peñacarrillo, M. L., González Gómez, Nieves|||/items/6c182060-e7f8-4e44-bd18-5fb98b24e841
Tipo de recurso: artículo
Fecha de publicación:2002
País:España
Institución:Universidad Alfonso X el Sabio
Repositorio:Repositorio Institucional de la Universidad Alfonso X el Sabio
Idioma:inglés
OAI Identifier:oai:archive.uax.com:20.500.12080/55085
Acceso en línea:https://hdl.handle.net/20.500.12080/55085
Access Level:acceso abierto
Palabra clave:GLP-1
Miocitos humanos
Glucosa
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spelling Glucagon-like peptide-1 (GLP-1) and glucose metabolism in human myocytes.Luque, M.A.Márquez, L.Acitores, A.Redondo, A.Morales, M.Valverde, I.Villanueva-Peñacarrillo, M. L.González Gómez, Nieves|||/items/6c182060-e7f8-4e44-bd18-5fb98b24e841GLP-1Miocitos humanosGlucosaGlucagon-like peptide-1 (GLP-1) has been shown to have insulin-like effects upon the metabolism of glucose in rat liver, muscle and fat, and on that of lipids in rat and human adipocytes. These actions seem to be exerted through specific receptors which, unlike that of the pancreas, are not - at least in liver and muscle - cAMP-associated. Here we have investigated the effect, its characteristics, and possible second messengers of GLP-1 on the glucose metabolism of human skeletal muscle, in tissue strips and primary cultured myocytes. In muscle strips, GLP-1, like insulin, stimulated glycogen synthesis, glycogen synthase a activity, and glucose oxidation and utilization, and inhibited glycogen phosphorylase a activity, all of this at physiological concentrations of the peptide. In cultured myotubes, GLP-1 exerted, from 10(-13) mol/l, a dose-related increase of the D-[U-(14)C]glucose incorporation into glycogen, with the same potency as insulin, together with an activation of glycogen synthase a; the effect of 10(-11) mol/l GLP-1 on both parameters was additive to that induced by the equimolar amount of insulin. Synthase a was still activated in cells after 2 days of exposure to GLP-1, as compared with myotubes maintained in the absence of peptide. In human muscle cells, exendin-4 and its truncated form 9-39 amide (Ex-9) are both agonists of the GLP-1 effect on glycogen synthesis and synthase a activity; but while neither GLP-1 nor exendin-4 affected the cellular cAMP content after 5-min incubation in the absence of 3-isobutyl-1-methylxantine (IBMX), an increase was detected with Ex-9. GLP-1, exendin-4, Ex-9 and insulin all induced the prompt hydrolysis of glycosylphosphatidylinositols (GPIs). This work shows a potent stimulatory effect of GLP-1 on the glucose metabolism of human skeletal muscle, and supports the long-term therapeutic value of the peptide. Further evidence for a GLP-1 receptor in this tissue, different from that of the pancreas, is also illustrated, suggesting a role for an inositolphosphoglycan (IPG) as at least one of the possible second messengers of the GLP-1 action in human muscle.BioscientificaUAX -Campus MadridUAX Archive20022002-01-0120022002-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12080/55085reponame:Repositorio Institucional de la Universidad Alfonso X el Sabioinstname:Universidad Alfonso X el SabioInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:archive.uax.com:20.500.12080/550852026-05-28T12:40:21Z
dc.title.none.fl_str_mv Glucagon-like peptide-1 (GLP-1) and glucose metabolism in human myocytes.
title Glucagon-like peptide-1 (GLP-1) and glucose metabolism in human myocytes.
spellingShingle Glucagon-like peptide-1 (GLP-1) and glucose metabolism in human myocytes.
Luque, M.A.
GLP-1
Miocitos humanos
Glucosa
title_short Glucagon-like peptide-1 (GLP-1) and glucose metabolism in human myocytes.
title_full Glucagon-like peptide-1 (GLP-1) and glucose metabolism in human myocytes.
title_fullStr Glucagon-like peptide-1 (GLP-1) and glucose metabolism in human myocytes.
title_full_unstemmed Glucagon-like peptide-1 (GLP-1) and glucose metabolism in human myocytes.
title_sort Glucagon-like peptide-1 (GLP-1) and glucose metabolism in human myocytes.
dc.creator.none.fl_str_mv Luque, M.A.
Márquez, L.
Acitores, A.
Redondo, A.
Morales, M.
Valverde, I.
Villanueva-Peñacarrillo, M. L.
González Gómez, Nieves|||/items/6c182060-e7f8-4e44-bd18-5fb98b24e841
author Luque, M.A.
author_facet Luque, M.A.
Márquez, L.
Acitores, A.
Redondo, A.
Morales, M.
Valverde, I.
Villanueva-Peñacarrillo, M. L.
González Gómez, Nieves|||/items/6c182060-e7f8-4e44-bd18-5fb98b24e841
author_role author
author2 Márquez, L.
Acitores, A.
Redondo, A.
Morales, M.
Valverde, I.
Villanueva-Peñacarrillo, M. L.
González Gómez, Nieves|||/items/6c182060-e7f8-4e44-bd18-5fb98b24e841
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UAX -Campus Madrid
UAX Archive
dc.subject.none.fl_str_mv GLP-1
Miocitos humanos
Glucosa
topic GLP-1
Miocitos humanos
Glucosa
description Glucagon-like peptide-1 (GLP-1) has been shown to have insulin-like effects upon the metabolism of glucose in rat liver, muscle and fat, and on that of lipids in rat and human adipocytes. These actions seem to be exerted through specific receptors which, unlike that of the pancreas, are not - at least in liver and muscle - cAMP-associated. Here we have investigated the effect, its characteristics, and possible second messengers of GLP-1 on the glucose metabolism of human skeletal muscle, in tissue strips and primary cultured myocytes. In muscle strips, GLP-1, like insulin, stimulated glycogen synthesis, glycogen synthase a activity, and glucose oxidation and utilization, and inhibited glycogen phosphorylase a activity, all of this at physiological concentrations of the peptide. In cultured myotubes, GLP-1 exerted, from 10(-13) mol/l, a dose-related increase of the D-[U-(14)C]glucose incorporation into glycogen, with the same potency as insulin, together with an activation of glycogen synthase a; the effect of 10(-11) mol/l GLP-1 on both parameters was additive to that induced by the equimolar amount of insulin. Synthase a was still activated in cells after 2 days of exposure to GLP-1, as compared with myotubes maintained in the absence of peptide. In human muscle cells, exendin-4 and its truncated form 9-39 amide (Ex-9) are both agonists of the GLP-1 effect on glycogen synthesis and synthase a activity; but while neither GLP-1 nor exendin-4 affected the cellular cAMP content after 5-min incubation in the absence of 3-isobutyl-1-methylxantine (IBMX), an increase was detected with Ex-9. GLP-1, exendin-4, Ex-9 and insulin all induced the prompt hydrolysis of glycosylphosphatidylinositols (GPIs). This work shows a potent stimulatory effect of GLP-1 on the glucose metabolism of human skeletal muscle, and supports the long-term therapeutic value of the peptide. Further evidence for a GLP-1 receptor in this tissue, different from that of the pancreas, is also illustrated, suggesting a role for an inositolphosphoglycan (IPG) as at least one of the possible second messengers of the GLP-1 action in human muscle.
publishDate 2002
dc.date.none.fl_str_mv 2002
2002-01-01
2002
2002-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12080/55085
url https://hdl.handle.net/20.500.12080/55085
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Bioscientifica
publisher.none.fl_str_mv Bioscientifica
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad Alfonso X el Sabio
instname:Universidad Alfonso X el Sabio
instname_str Universidad Alfonso X el Sabio
reponame_str Repositorio Institucional de la Universidad Alfonso X el Sabio
collection Repositorio Institucional de la Universidad Alfonso X el Sabio
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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