Medicated scaffolds prepared with hydroxyapatite/streptomycin nanoparticles encapsulated into polylactide microfibers

The preparation, characterization, and controlled release of hydroxyapatite (HAp) nanoparticles loaded with streptomycin (STR) was studied. These nanoparticles are highly appropriate for the treatment of bacterial infections and are also promising for the treatment of cancer cells. The analyses invo...

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Autores: Kadkhodaie Elyaderani, Amirmajid, Lama Odría, María del Carmen Elizabeth de|||0000-0002-5076-8312, Rivas Cañas, Manuel|||0000-0002-8884-3410, Martínez Rovira, Immaculada|||0000-0002-2918-489X, Yousef, Ibraheem, Puiggalí Allepuz, Jordi, Valle Mendoza, Luis Javier del|||0000-0001-9916-1741
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/369797
Acceso en línea:https://hdl.handle.net/2117/369797
https://dx.doi.org/10.3390/ijms23031282
Access Level:acceso abierto
Palabra clave:Nanoparticles
Hydroxyapatite nanoparticles
Streptomycin
Polylactide
Drug delivery
Antimicrobial activity
Cytotoxicity
Nanopartícules
Àrees temàtiques de la UPC::Enginyeria química
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spelling Medicated scaffolds prepared with hydroxyapatite/streptomycin nanoparticles encapsulated into polylactide microfibersKadkhodaie Elyaderani, AmirmajidLama Odría, María del Carmen Elizabeth de|||0000-0002-5076-8312Rivas Cañas, Manuel|||0000-0002-8884-3410Martínez Rovira, Immaculada|||0000-0002-2918-489XYousef, IbraheemPuiggalí Allepuz, JordiValle Mendoza, Luis Javier del|||0000-0001-9916-1741NanoparticlesHydroxyapatite nanoparticlesStreptomycinPolylactideDrug deliveryAntimicrobial activityCytotoxicityNanopartículesÀrees temàtiques de la UPC::Enginyeria químicaThe preparation, characterization, and controlled release of hydroxyapatite (HAp) nanoparticles loaded with streptomycin (STR) was studied. These nanoparticles are highly appropriate for the treatment of bacterial infections and are also promising for the treatment of cancer cells. The analyses involved scanning electron microscopy, dynamic light scattering (DLS) and Z-potential measurements, as well as infrared spectroscopy and X-ray diffraction. Both amorphous (ACP) and crystalline (cHAp) hydroxyapatite nanoparticles were considered since they differ in their release behavior (faster and slower for amorphous and crystalline particles, respectively). The encapsulated nanoparticles were finally incorporated into biodegradable and biocompatible polylactide (PLA) scaffolds. The STR load was carried out following different pathways during the synthesis/precipitation of the nanoparticles (i.e., nucleation steps) and also by simple adsorption once the nanoparticles were formed. The loaded nanoparticles were biocompatible according to the study of the cytotoxicity of extracts using different cell lines. FTIR microspectroscopy was also employed to evaluate the cytotoxic effect on cancer cell lines of nanoparticles internalized by endocytosis. The results were promising when amorphous nanoparticles were employed. The nanoparticles loaded with STR increased their size and changed their superficial negative charge to positive. The nanoparticles’ crystallinity decreased, with the consequence that their crystal sizes reduced, when STR was incorporated into their structure. STR maintained its antibacterial activity, although it was reduced during the adsorption into the nanoparticles formed. The STR release was faster from the amorphous ACP nanoparticles and slower from the crystalline cHAp nanoparticles. However, in both cases, the STR release was slower when incorporated in calcium and phosphate during the synthesis. The biocompatibility of these nanoparticles was assayed by two approximations. When extracts from the nanoparticles were evaluated in cultures of cell lines, no cytotoxic damage was observed at concentrations of less than 10 mg/mL. This demonstrated their biocompatibility. Another experiment using FTIR microspectroscopy evaluated the cytotoxic effect of nanoparticles internalized by endocytosis in cancer cells. The results demonstrated slight damage to the biomacromolecules when the cells were treated with ACP nanoparticles. Both ACP and cHAp nanoparticles were efficiently encapsulated in PLA electrospun matrices, providing functionality and bioactive properties.Peer Reviewed20222022-01-2420222022-07-07journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/369797https://dx.doi.org/10.3390/ijms23031282reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3697972026-05-27T15:37:01Z
dc.title.none.fl_str_mv Medicated scaffolds prepared with hydroxyapatite/streptomycin nanoparticles encapsulated into polylactide microfibers
title Medicated scaffolds prepared with hydroxyapatite/streptomycin nanoparticles encapsulated into polylactide microfibers
spellingShingle Medicated scaffolds prepared with hydroxyapatite/streptomycin nanoparticles encapsulated into polylactide microfibers
Kadkhodaie Elyaderani, Amirmajid
Nanoparticles
Hydroxyapatite nanoparticles
Streptomycin
Polylactide
Drug delivery
Antimicrobial activity
Cytotoxicity
Nanopartícules
Àrees temàtiques de la UPC::Enginyeria química
title_short Medicated scaffolds prepared with hydroxyapatite/streptomycin nanoparticles encapsulated into polylactide microfibers
title_full Medicated scaffolds prepared with hydroxyapatite/streptomycin nanoparticles encapsulated into polylactide microfibers
title_fullStr Medicated scaffolds prepared with hydroxyapatite/streptomycin nanoparticles encapsulated into polylactide microfibers
title_full_unstemmed Medicated scaffolds prepared with hydroxyapatite/streptomycin nanoparticles encapsulated into polylactide microfibers
title_sort Medicated scaffolds prepared with hydroxyapatite/streptomycin nanoparticles encapsulated into polylactide microfibers
dc.creator.none.fl_str_mv Kadkhodaie Elyaderani, Amirmajid
Lama Odría, María del Carmen Elizabeth de|||0000-0002-5076-8312
Rivas Cañas, Manuel|||0000-0002-8884-3410
Martínez Rovira, Immaculada|||0000-0002-2918-489X
Yousef, Ibraheem
Puiggalí Allepuz, Jordi
Valle Mendoza, Luis Javier del|||0000-0001-9916-1741
author Kadkhodaie Elyaderani, Amirmajid
author_facet Kadkhodaie Elyaderani, Amirmajid
Lama Odría, María del Carmen Elizabeth de|||0000-0002-5076-8312
Rivas Cañas, Manuel|||0000-0002-8884-3410
Martínez Rovira, Immaculada|||0000-0002-2918-489X
Yousef, Ibraheem
Puiggalí Allepuz, Jordi
Valle Mendoza, Luis Javier del|||0000-0001-9916-1741
author_role author
author2 Lama Odría, María del Carmen Elizabeth de|||0000-0002-5076-8312
Rivas Cañas, Manuel|||0000-0002-8884-3410
Martínez Rovira, Immaculada|||0000-0002-2918-489X
Yousef, Ibraheem
Puiggalí Allepuz, Jordi
Valle Mendoza, Luis Javier del|||0000-0001-9916-1741
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanoparticles
Hydroxyapatite nanoparticles
Streptomycin
Polylactide
Drug delivery
Antimicrobial activity
Cytotoxicity
Nanopartícules
Àrees temàtiques de la UPC::Enginyeria química
topic Nanoparticles
Hydroxyapatite nanoparticles
Streptomycin
Polylactide
Drug delivery
Antimicrobial activity
Cytotoxicity
Nanopartícules
Àrees temàtiques de la UPC::Enginyeria química
description The preparation, characterization, and controlled release of hydroxyapatite (HAp) nanoparticles loaded with streptomycin (STR) was studied. These nanoparticles are highly appropriate for the treatment of bacterial infections and are also promising for the treatment of cancer cells. The analyses involved scanning electron microscopy, dynamic light scattering (DLS) and Z-potential measurements, as well as infrared spectroscopy and X-ray diffraction. Both amorphous (ACP) and crystalline (cHAp) hydroxyapatite nanoparticles were considered since they differ in their release behavior (faster and slower for amorphous and crystalline particles, respectively). The encapsulated nanoparticles were finally incorporated into biodegradable and biocompatible polylactide (PLA) scaffolds. The STR load was carried out following different pathways during the synthesis/precipitation of the nanoparticles (i.e., nucleation steps) and also by simple adsorption once the nanoparticles were formed. The loaded nanoparticles were biocompatible according to the study of the cytotoxicity of extracts using different cell lines. FTIR microspectroscopy was also employed to evaluate the cytotoxic effect on cancer cell lines of nanoparticles internalized by endocytosis. The results were promising when amorphous nanoparticles were employed. The nanoparticles loaded with STR increased their size and changed their superficial negative charge to positive. The nanoparticles’ crystallinity decreased, with the consequence that their crystal sizes reduced, when STR was incorporated into their structure. STR maintained its antibacterial activity, although it was reduced during the adsorption into the nanoparticles formed. The STR release was faster from the amorphous ACP nanoparticles and slower from the crystalline cHAp nanoparticles. However, in both cases, the STR release was slower when incorporated in calcium and phosphate during the synthesis. The biocompatibility of these nanoparticles was assayed by two approximations. When extracts from the nanoparticles were evaluated in cultures of cell lines, no cytotoxic damage was observed at concentrations of less than 10 mg/mL. This demonstrated their biocompatibility. Another experiment using FTIR microspectroscopy evaluated the cytotoxic effect of nanoparticles internalized by endocytosis in cancer cells. The results demonstrated slight damage to the biomacromolecules when the cells were treated with ACP nanoparticles. Both ACP and cHAp nanoparticles were efficiently encapsulated in PLA electrospun matrices, providing functionality and bioactive properties.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-24
2022
2022-07-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/369797
https://dx.doi.org/10.3390/ijms23031282
url https://hdl.handle.net/2117/369797
https://dx.doi.org/10.3390/ijms23031282
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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