Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes

Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell fractions...

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Autores: Gañán-Gómez, I. (Irene)|||/items/92d1787d-8a7f-414a-af8a-a0cc2094a8af, Clise-Dwyer, K. (Karen)|||/items/c0664b53-8e96-4c11-aa0d-58c0afb8baee, Colla, S. (Simona)|||/items/659f9e93-5695-4c4a-948e-85565d696acc
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/121485
Acesso em linha:https://hdl.handle.net/10171/121485
Access Level:acceso abierto
Palavra-chave:hematopoietic stem and progenitor cells (HSPCs)
myelodysplastic syndromes (MDS)
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repository_id_str
spelling Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromesGañán-Gómez, I. (Irene)|||/items/92d1787d-8a7f-414a-af8a-a0cc2094a8afClise-Dwyer, K. (Karen)|||/items/c0664b53-8e96-4c11-aa0d-58c0afb8baeeColla, S. (Simona)|||/items/659f9e93-5695-4c4a-948e-85565d696acchematopoietic stem and progenitor cells (HSPCs)myelodysplastic syndromes (MDS)Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell fractions and their culturing with the support of stromal cells for improved maintenance during drug testing. Lastly, specific steps are given to quantify surviving cells and assess changes in the HSPC hierarchies.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/121485reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/1214852026-06-21T12:47:57Z
dc.title.none.fl_str_mv Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
spellingShingle Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
Gañán-Gómez, I. (Irene)|||/items/92d1787d-8a7f-414a-af8a-a0cc2094a8af
hematopoietic stem and progenitor cells (HSPCs)
myelodysplastic syndromes (MDS)
title_short Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title_full Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title_fullStr Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title_full_unstemmed Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
title_sort Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
dc.creator.none.fl_str_mv Gañán-Gómez, I. (Irene)|||/items/92d1787d-8a7f-414a-af8a-a0cc2094a8af
Clise-Dwyer, K. (Karen)|||/items/c0664b53-8e96-4c11-aa0d-58c0afb8baee
Colla, S. (Simona)|||/items/659f9e93-5695-4c4a-948e-85565d696acc
author Gañán-Gómez, I. (Irene)|||/items/92d1787d-8a7f-414a-af8a-a0cc2094a8af
author_facet Gañán-Gómez, I. (Irene)|||/items/92d1787d-8a7f-414a-af8a-a0cc2094a8af
Clise-Dwyer, K. (Karen)|||/items/c0664b53-8e96-4c11-aa0d-58c0afb8baee
Colla, S. (Simona)|||/items/659f9e93-5695-4c4a-948e-85565d696acc
author_role author
author2 Clise-Dwyer, K. (Karen)|||/items/c0664b53-8e96-4c11-aa0d-58c0afb8baee
Colla, S. (Simona)|||/items/659f9e93-5695-4c4a-948e-85565d696acc
author2_role author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv hematopoietic stem and progenitor cells (HSPCs)
myelodysplastic syndromes (MDS)
topic hematopoietic stem and progenitor cells (HSPCs)
myelodysplastic syndromes (MDS)
description Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell fractions and their culturing with the support of stromal cells for improved maintenance during drug testing. Lastly, specific steps are given to quantify surviving cells and assess changes in the HSPC hierarchies.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/121485
url https://hdl.handle.net/10171/121485
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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