Chronic central leptin infusion promotes an anti‐inflammatory cytokine profile related to the activation of insulin signaling in the gastrocnemius of male rats

Leptin is involved in the modulation of insulin signaling in peripheral tissues, being closely associated with changes in lipid metabolism. This adipokine modifies inflammatory pathways that can interact with insulin targets in peripheral organs; however, the mechanisms remain unclear. Inflammatory...

Descripción completa

Detalles Bibliográficos
Autores: Barrios, Vicente, Guerra-Cantera, Santiago, Martín-Rivada, Álvaro, Canelles, Sandra, Campillo-Calatayud, Ana, Arilla-Ferreiro, Eduardo, Frago Fernández, Laura María, Chowen, Julie Ann, Argente Oliver, Jesús
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/706750
Acceso en línea:http://hdl.handle.net/10486/706750
https://dx.doi.org/10.3390/biomedicines10071465
Access Level:acceso abierto
Palabra clave:adiponectin
cytokine
gastrocnemius
inflammation
leptin
NEFA
signaling
Medicina
id ES_f62a9fa3b9e9feafe67fdd8d8f67e4fd
oai_identifier_str oai:repositorio.uam.es:10486/706750
network_acronym_str ES
network_name_str España
repository_id_str
spelling Chronic central leptin infusion promotes an anti‐inflammatory cytokine profile related to the activation of insulin signaling in the gastrocnemius of male ratsBarrios, VicenteGuerra-Cantera, SantiagoMartín-Rivada, ÁlvaroCanelles, SandraCampillo-Calatayud, AnaArilla-Ferreiro, EduardoFrago Fernández, Laura MaríaChowen, Julie AnnArgente Oliver, JesúsadiponectincytokinegastrocnemiusinflammationleptinNEFAsignalingMedicinaLeptin is involved in the modulation of insulin signaling in peripheral tissues, being closely associated with changes in lipid metabolism. This adipokine modifies inflammatory pathways that can interact with insulin targets in peripheral organs; however, the mechanisms remain unclear. Inflammatory and insulin signaling targets, cytokines, adiponectin, irisin and non‐esteri-fied fatty acid (NEFA) levels and enzymes of fatty acid anabolism were studied in the gastrocnem-ius of chronic centrally infused leptin (L), pair‐fed and control rats. The phosphorylation of signal transducer and activator of transcription 3 (STAT3) and c‐Jun N‐terminal kinase (JNK) was reduced in L rats (59% and 58%, respectively). The phosphorylation of the insulin receptor and Akt and adiponectin and irisin content was increased in L rats (154%, 157%, 308% and 329%, respectively). The levels of glucose‐6‐phosphate dehydrogenase, the mRNA content of acetyl Co‐A carboxylase and NEFA concentrations were diminished in the muscles of L rats (59%, 50% and 61%, respec-tively). The activation of JNK correlated positively with STAT3 phosphorylation, tumoral necrosis factor‐α and NEFA and negatively with irisin and Akt phosphorylation. These data suggest that the activation of insulin signaling targets and a decrease in NEFA content are associated with a reduction in muscle inflammation parameters, suggesting that leptin may integrate these pathwaysThis research was funded by the Spanish Ministry of Science and Innovation with the help of European FEDER funding (FIS PI19/00166), Ministerio de Economía y Competitividad (BFU2017-82565-C2-1-R) and the Network Center for Biomedical Research on Obesity and Nutrition (CIBEROBN) Instituto Carlos III. S.C. was supported by CIBER de Fisiopatología de la Obesidad y la Nutrición, Instituto de Salud Carlos III and A.C.-C. by the Comunidad de Madrid (PEJ-2019-TL_BMD-13560)MDPIDepartamento de PediatríaFacultad de Medicina20222022-06-21research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/706750https://dx.doi.org/10.3390/biomedicines10071465reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7067502026-06-23T12:46:27Z
dc.title.none.fl_str_mv Chronic central leptin infusion promotes an anti‐inflammatory cytokine profile related to the activation of insulin signaling in the gastrocnemius of male rats
title Chronic central leptin infusion promotes an anti‐inflammatory cytokine profile related to the activation of insulin signaling in the gastrocnemius of male rats
spellingShingle Chronic central leptin infusion promotes an anti‐inflammatory cytokine profile related to the activation of insulin signaling in the gastrocnemius of male rats
Barrios, Vicente
adiponectin
cytokine
gastrocnemius
inflammation
leptin
NEFA
signaling
Medicina
title_short Chronic central leptin infusion promotes an anti‐inflammatory cytokine profile related to the activation of insulin signaling in the gastrocnemius of male rats
title_full Chronic central leptin infusion promotes an anti‐inflammatory cytokine profile related to the activation of insulin signaling in the gastrocnemius of male rats
title_fullStr Chronic central leptin infusion promotes an anti‐inflammatory cytokine profile related to the activation of insulin signaling in the gastrocnemius of male rats
title_full_unstemmed Chronic central leptin infusion promotes an anti‐inflammatory cytokine profile related to the activation of insulin signaling in the gastrocnemius of male rats
title_sort Chronic central leptin infusion promotes an anti‐inflammatory cytokine profile related to the activation of insulin signaling in the gastrocnemius of male rats
dc.creator.none.fl_str_mv Barrios, Vicente
Guerra-Cantera, Santiago
Martín-Rivada, Álvaro
Canelles, Sandra
Campillo-Calatayud, Ana
Arilla-Ferreiro, Eduardo
Frago Fernández, Laura María
Chowen, Julie Ann
Argente Oliver, Jesús
author Barrios, Vicente
author_facet Barrios, Vicente
Guerra-Cantera, Santiago
Martín-Rivada, Álvaro
Canelles, Sandra
Campillo-Calatayud, Ana
Arilla-Ferreiro, Eduardo
Frago Fernández, Laura María
Chowen, Julie Ann
Argente Oliver, Jesús
author_role author
author2 Guerra-Cantera, Santiago
Martín-Rivada, Álvaro
Canelles, Sandra
Campillo-Calatayud, Ana
Arilla-Ferreiro, Eduardo
Frago Fernández, Laura María
Chowen, Julie Ann
Argente Oliver, Jesús
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Pediatría
Facultad de Medicina
dc.subject.none.fl_str_mv adiponectin
cytokine
gastrocnemius
inflammation
leptin
NEFA
signaling
Medicina
topic adiponectin
cytokine
gastrocnemius
inflammation
leptin
NEFA
signaling
Medicina
description Leptin is involved in the modulation of insulin signaling in peripheral tissues, being closely associated with changes in lipid metabolism. This adipokine modifies inflammatory pathways that can interact with insulin targets in peripheral organs; however, the mechanisms remain unclear. Inflammatory and insulin signaling targets, cytokines, adiponectin, irisin and non‐esteri-fied fatty acid (NEFA) levels and enzymes of fatty acid anabolism were studied in the gastrocnem-ius of chronic centrally infused leptin (L), pair‐fed and control rats. The phosphorylation of signal transducer and activator of transcription 3 (STAT3) and c‐Jun N‐terminal kinase (JNK) was reduced in L rats (59% and 58%, respectively). The phosphorylation of the insulin receptor and Akt and adiponectin and irisin content was increased in L rats (154%, 157%, 308% and 329%, respectively). The levels of glucose‐6‐phosphate dehydrogenase, the mRNA content of acetyl Co‐A carboxylase and NEFA concentrations were diminished in the muscles of L rats (59%, 50% and 61%, respec-tively). The activation of JNK correlated positively with STAT3 phosphorylation, tumoral necrosis factor‐α and NEFA and negatively with irisin and Akt phosphorylation. These data suggest that the activation of insulin signaling targets and a decrease in NEFA content are associated with a reduction in muscle inflammation parameters, suggesting that leptin may integrate these pathways
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-06-21
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/706750
https://dx.doi.org/10.3390/biomedicines10071465
url http://hdl.handle.net/10486/706750
https://dx.doi.org/10.3390/biomedicines10071465
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424711114424320
score 15.301603

Similares en LA Referencia