Cell-driven encapsulation of chlorophyllin-based carbon dots within exosomes for enhanced photodynamic therapy: miRNA profiling reveals mechanistic insights

et al.

Detalles Bibliográficos
Autores: Besbinar, Omur, Martín-Pardillos, Ana, Hueso, José L., Santamaría, Jesús, Yilmazer, Açelya
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/413421
Acceso en línea:http://hdl.handle.net/10261/413421
Access Level:acceso abierto
Palabra clave:Exosomes
Drug delivery
Carbondots
miRNA profiling
Photodynamic therapy
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spelling Cell-driven encapsulation of chlorophyllin-based carbon dots within exosomes for enhanced photodynamic therapy: miRNA profiling reveals mechanistic insightsBesbinar, OmurMartín-Pardillos, AnaHueso, José L.Santamaría, JesúsYilmazer, AçelyaExosomesDrug deliveryCarbondotsmiRNA profilingPhotodynamic therapyet al.Exosomes, nanoscale extracellular vesicles, have emerged as promising carriers in drug delivery due to their ability to bypass biological barriers, low toxicity, high stability, and intrinsic targeting capabilities. Mesenchymal stem-cell-derived exosomes (EXOMSC), with their natural tropism toward the tumor microenvironment, offer an ideal platform for enhancing therapeutic cargo delivery. In this study, we demonstrate an approach where red-emission chlorophyll-based carbon dots (Chl-CDs) were encapsulated within EXOMSC through a cell-driven uptake mechanism, creating CD@EXOMSC. These exosomes achieved superior photodynamic therapy (PDT) efficacy, requiring 40 times less nanomaterial compared to freestanding Chl-CDs. Mechanistic insights from glioblastoma cell miRNA profiling revealed that the enhanced efficacy was mediated by the regulation of efflux transporter genes, oxidative stress responses, and endocytosis pathways. This work highlights the synergistic potential of combining photosensitizers and miRNA-rich exosomes to achieve targeted and sustained therapeutic delivery, paving the way for a multifaceted approach in cancer therapy.O.B. expresses gratitude to the Council of Higher Education (YOK) for the YOK100/2000 Ph.D. scholarship and to the Scientific and Technological Research Council of Turkey (TUBITAK) for the 2214-A PhD. International Research Scholarship. A.Y. acknowledges funding from the European Union’s Horizon Europe program, under the Marie Sklodowska-Curie grant agreement No. 101086184 (MX-MAP). J.L.H. and J.S. acknowledge the Spanish Research Agency and MICN/AEI (CNS2022-135911), the Government of Aragon (Group T57_23R) and CIBER-BBN. Ayuda Severo Ochoa CEX2023-001286-S financiada por MICIU/AEI/10.13039/501100011033 is also acknowledged. J.S., A.M.P., and J.L.H. acknowledge funding from Ministerio de Ciencia, Innovación y Universidades, Project CONCERT, PID2023-148732NB-I00.With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2023-001286-S).Peer reviewedAmerican Chemical SocietyCouncil of Higher Education (Türkiye)The Scientific and Technological Research Council of TürkiyeEuropean CommissionGobierno de AragónAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/413421reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/HE/101086184info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CNS2022-135911info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2023-001286-Sinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-148732NB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1021/acsami.5c18555https://doi.org/10.1021/acsami.5c18555Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4134212026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cell-driven encapsulation of chlorophyllin-based carbon dots within exosomes for enhanced photodynamic therapy: miRNA profiling reveals mechanistic insights
title Cell-driven encapsulation of chlorophyllin-based carbon dots within exosomes for enhanced photodynamic therapy: miRNA profiling reveals mechanistic insights
spellingShingle Cell-driven encapsulation of chlorophyllin-based carbon dots within exosomes for enhanced photodynamic therapy: miRNA profiling reveals mechanistic insights
Besbinar, Omur
Exosomes
Drug delivery
Carbondots
miRNA profiling
Photodynamic therapy
title_short Cell-driven encapsulation of chlorophyllin-based carbon dots within exosomes for enhanced photodynamic therapy: miRNA profiling reveals mechanistic insights
title_full Cell-driven encapsulation of chlorophyllin-based carbon dots within exosomes for enhanced photodynamic therapy: miRNA profiling reveals mechanistic insights
title_fullStr Cell-driven encapsulation of chlorophyllin-based carbon dots within exosomes for enhanced photodynamic therapy: miRNA profiling reveals mechanistic insights
title_full_unstemmed Cell-driven encapsulation of chlorophyllin-based carbon dots within exosomes for enhanced photodynamic therapy: miRNA profiling reveals mechanistic insights
title_sort Cell-driven encapsulation of chlorophyllin-based carbon dots within exosomes for enhanced photodynamic therapy: miRNA profiling reveals mechanistic insights
dc.creator.none.fl_str_mv Besbinar, Omur
Martín-Pardillos, Ana
Hueso, José L.
Santamaría, Jesús
Yilmazer, Açelya
author Besbinar, Omur
author_facet Besbinar, Omur
Martín-Pardillos, Ana
Hueso, José L.
Santamaría, Jesús
Yilmazer, Açelya
author_role author
author2 Martín-Pardillos, Ana
Hueso, José L.
Santamaría, Jesús
Yilmazer, Açelya
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Council of Higher Education (Türkiye)
The Scientific and Technological Research Council of Türkiye
European Commission
Gobierno de Aragón
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Exosomes
Drug delivery
Carbondots
miRNA profiling
Photodynamic therapy
topic Exosomes
Drug delivery
Carbondots
miRNA profiling
Photodynamic therapy
description et al.
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/413421
url http://hdl.handle.net/10261/413421
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/HE/101086184
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CNS2022-135911
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2023-001286-S
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-148732NB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1021/acsami.5c18555
https://doi.org/10.1021/acsami.5c18555
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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