Role of tactic response on the mobilization of motile bacteria through micrometer-sized pores

10 páginas.- 6 figuras.- 1 tabla.- referencias .- . Supplementary data Figures showing the FIB-SEM images of the membrane bioreactor experiments, the tactic response of Pseudomonas putida G7 under sonication conditions in transport assays and capillary test and bioreactor transport experiments with...

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Detalles Bibliográficos
Autores: Castilla-Alcántara, José Carlos, Akbari, A., Ghoshal, S., Ortega Calvo, J. J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/271396
Acceso en línea:http://hdl.handle.net/10261/271396
Access Level:acceso abierto
Palabra clave:Bacterial motility
Taxis
Pore
Contaminant
Bioaccessibility
Soil
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spelling Role of tactic response on the mobilization of motile bacteria through micrometer-sized poresCastilla-Alcántara, José CarlosAkbari, A.Ghoshal, S.Ortega Calvo, J. J.Bacterial motilityTaxisPoreContaminantBioaccessibilitySoil10 páginas.- 6 figuras.- 1 tabla.- referencias .- . Supplementary data Figures showing the FIB-SEM images of the membrane bioreactor experiments, the tactic response of Pseudomonas putida G7 under sonication conditions in transport assays and capillary test and bioreactor transport experiments with different concentrations of the chemoeffectors. Tables showing the experimental growth control with chemical effectors and bacterial accessibility to the different porous fractions of contaminated soil. Supplementary data to this article can be found online at: doi.org/10.1016/j.scitotenv.2022.154938A major cause of high bioremediation endpoints is the limited bioaccessibility to residual contaminants resting in soil pores with diameters close to the size exclusion limit of bacterial cells. Under nongrowing conditions and in the absence of hydraulic flow, we examined how the tactic behavior of motile, contaminant-degrading Pseudomonas putida G7 cells (2 × 1 μm) influenced passage through membranes with pores ranging in size from 1 μm to 12 μm. The bacteria were spontaneously retained by the membranes - even those with the largest pore size. However, the cells were mobilized through 5 μm and 12 μm pores after the application of an attractant (salicylate). Mobilization also occurred by attraction to the common root exudate constituents γ-aminobutyric acid and citrate and repellence (or negative taxis) to zero-valent iron nanoparticles. The observed pore size threshold for tactic mobilization (5 μm) and unaltered cell fluxes and effective cell diffusion against different chemoeffector strengths and concentrations suggest that there is a physical constraint on the gradient sensing mechanism at the pores that drives the tactic response. Our results indicate that chemically mediated, small-scale tactic reactions of motile bacteria may become relevant to enhance the bioaccessibility of the residual contaminants present in micrometer-sized soil pores.We would like to thank the Spanish Ministries of Economy and Competitiveness (CGL2016-77497-R) and Science and Innovation (BES-2017-079905 and PID2019-109700RB-C21) for supporting this workPeer reviewedElsevierMinisterio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)Castilla-Alcántara, José Carlos [0000-0002-9123-1052]Ghoshal, S. [0000-0001-9968-6150]Ortega Calvo, J. J. [0000-0003-1672-5199]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/271396reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2016-77497-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109700RB-C21info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BES-2017-079905http://dx.doi.org/10.1016/j.scitotenv.2022.154938Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2713962026-05-22T06:33:51Z
dc.title.none.fl_str_mv Role of tactic response on the mobilization of motile bacteria through micrometer-sized pores
title Role of tactic response on the mobilization of motile bacteria through micrometer-sized pores
spellingShingle Role of tactic response on the mobilization of motile bacteria through micrometer-sized pores
Castilla-Alcántara, José Carlos
Bacterial motility
Taxis
Pore
Contaminant
Bioaccessibility
Soil
title_short Role of tactic response on the mobilization of motile bacteria through micrometer-sized pores
title_full Role of tactic response on the mobilization of motile bacteria through micrometer-sized pores
title_fullStr Role of tactic response on the mobilization of motile bacteria through micrometer-sized pores
title_full_unstemmed Role of tactic response on the mobilization of motile bacteria through micrometer-sized pores
title_sort Role of tactic response on the mobilization of motile bacteria through micrometer-sized pores
dc.creator.none.fl_str_mv Castilla-Alcántara, José Carlos
Akbari, A.
Ghoshal, S.
Ortega Calvo, J. J.
author Castilla-Alcántara, José Carlos
author_facet Castilla-Alcántara, José Carlos
Akbari, A.
Ghoshal, S.
Ortega Calvo, J. J.
author_role author
author2 Akbari, A.
Ghoshal, S.
Ortega Calvo, J. J.
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Castilla-Alcántara, José Carlos [0000-0002-9123-1052]
Ghoshal, S. [0000-0001-9968-6150]
Ortega Calvo, J. J. [0000-0003-1672-5199]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacterial motility
Taxis
Pore
Contaminant
Bioaccessibility
Soil
topic Bacterial motility
Taxis
Pore
Contaminant
Bioaccessibility
Soil
description 10 páginas.- 6 figuras.- 1 tabla.- referencias .- . Supplementary data Figures showing the FIB-SEM images of the membrane bioreactor experiments, the tactic response of Pseudomonas putida G7 under sonication conditions in transport assays and capillary test and bioreactor transport experiments with different concentrations of the chemoeffectors. Tables showing the experimental growth control with chemical effectors and bacterial accessibility to the different porous fractions of contaminated soil. Supplementary data to this article can be found online at: doi.org/10.1016/j.scitotenv.2022.154938
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/271396
url http://hdl.handle.net/10261/271396
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2016-77497-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109700RB-C21
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BES-2017-079905
http://dx.doi.org/10.1016/j.scitotenv.2022.154938
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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