Titration of proteinase K digestion of the brain PrPres from MoPrP H95Y mice

Immunoblotting of brain PrPres from k905 mice and an atypical scrapie Nor98 control (C+ Atyp Sc) digested with a range of increasing concentration of PK (from 0 to 120 μg/ml), revealed with 12B2 Mab. Dilution factor of the loaded samples is provided. Molecular weight markers in kilodaltons (kDa) are...

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Detalles Bibliográficos
Autores: Torres, Juan María, Marín-Moreno, Alba, Espinosa, Juan Carlos, Canoyra, Sara, Burato, Anna, Ciullini, Arianna, De Luca, Chiara Maria Giulia, Bistaffa, Edoardo, Moda, F., Legname, Giuseppe
Tipo de recurso: conjunto de datos
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/406754
Acceso en línea:http://hdl.handle.net/10261/406754
Access Level:acceso abierto
Palabra clave:Neurodegenerative disorders caused
Mice expressing wild
Copper may play
div >< p
vivo </
tga20 </
Spongiform encephalopathy
Spongiform degeneration
Region located
Prion protein
Prion diseases
Octapeptide repeats
Misfolded isoform
Histidine 95
Functional implication
Brain homogenate
Binding site
100 Days
Descripción
Sumario:Immunoblotting of brain PrPres from k905 mice and an atypical scrapie Nor98 control (C+ Atyp Sc) digested with a range of increasing concentration of PK (from 0 to 120 μg/ml), revealed with 12B2 Mab. Dilution factor of the loaded samples is provided. Molecular weight markers in kilodaltons (kDa) are included on the left side of the blots.

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