Analysis of the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy

data showed that treatment of rats with a tachykinin NK3R antagonist caused a reduction of litter size. In the present study, we analyzed the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy. Uterine samples were obtained from early pregnant rats (Days 1-9...

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Autores: Pinto Pérez, Francisco M., Bello, Aixa R., Gallardo-Castro, Manuel, Valladares, Francisco, Almeida, Teresa A., Tena-Sempere, Manuel, Candenas, M. Luz
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/124344
Acceso en línea:http://hdl.handle.net/10261/124344
Access Level:acceso abierto
Palabra clave:Decidua
Early pregnancy
Rat uterus
Tachykinin receptors
Tachykinins
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spelling Analysis of the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancyPinto Pérez, Francisco M.Bello, Aixa R.Gallardo-Castro, ManuelValladares, FranciscoAlmeida, Teresa A.Tena-Sempere, ManuelCandenas, M. LuzDeciduaEarly pregnancyRat uterusTachykinin receptorsTachykininsdata showed that treatment of rats with a tachykinin NK3R antagonist caused a reduction of litter size. In the present study, we analyzed the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy. Uterine samples were obtained from early pregnant rats (Days 1-9 of pregnancy) and from nonpregnant rats during the proestrus stage of the ovarian cycle, and real-time quantitative RT-PCR, immunohistochemistry, and Western blot studies were used to investigate the pattern of expression of tachykinins and tachykinin receptors. We found that all tachykinins and tachykinin receptors were locally synthesized in the uterus of early pregnant rats. The expression of substance P, neurokinin B, and the tachykinin receptors NK1R and NK3R mRNAs and proteins underwent major changes during the days around implantation and they were widely distributed in implantation sites, being particularly abundant in decidual cells. These findings support the involvement of the tachykinin system in the series of uterine events that occur around embryo implantation in the rat.Peer reviewedSociety for the Study of ReproductionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/124344reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1095/biolreprod.115.130617Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1243442026-05-22T06:33:51Z
dc.title.none.fl_str_mv Analysis of the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy
title Analysis of the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy
spellingShingle Analysis of the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy
Pinto Pérez, Francisco M.
Decidua
Early pregnancy
Rat uterus
Tachykinin receptors
Tachykinins
title_short Analysis of the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy
title_full Analysis of the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy
title_fullStr Analysis of the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy
title_full_unstemmed Analysis of the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy
title_sort Analysis of the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy
dc.creator.none.fl_str_mv Pinto Pérez, Francisco M.
Bello, Aixa R.
Gallardo-Castro, Manuel
Valladares, Francisco
Almeida, Teresa A.
Tena-Sempere, Manuel
Candenas, M. Luz
author Pinto Pérez, Francisco M.
author_facet Pinto Pérez, Francisco M.
Bello, Aixa R.
Gallardo-Castro, Manuel
Valladares, Francisco
Almeida, Teresa A.
Tena-Sempere, Manuel
Candenas, M. Luz
author_role author
author2 Bello, Aixa R.
Gallardo-Castro, Manuel
Valladares, Francisco
Almeida, Teresa A.
Tena-Sempere, Manuel
Candenas, M. Luz
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Decidua
Early pregnancy
Rat uterus
Tachykinin receptors
Tachykinins
topic Decidua
Early pregnancy
Rat uterus
Tachykinin receptors
Tachykinins
description data showed that treatment of rats with a tachykinin NK3R antagonist caused a reduction of litter size. In the present study, we analyzed the expression of tachykinins and tachykinin receptors in the rat uterus during early pregnancy. Uterine samples were obtained from early pregnant rats (Days 1-9 of pregnancy) and from nonpregnant rats during the proestrus stage of the ovarian cycle, and real-time quantitative RT-PCR, immunohistochemistry, and Western blot studies were used to investigate the pattern of expression of tachykinins and tachykinin receptors. We found that all tachykinins and tachykinin receptors were locally synthesized in the uterus of early pregnant rats. The expression of substance P, neurokinin B, and the tachykinin receptors NK1R and NK3R mRNAs and proteins underwent major changes during the days around implantation and they were widely distributed in implantation sites, being particularly abundant in decidual cells. These findings support the involvement of the tachykinin system in the series of uterine events that occur around embryo implantation in the rat.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/124344
url http://hdl.handle.net/10261/124344
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1095/biolreprod.115.130617
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for the Study of Reproduction
publisher.none.fl_str_mv Society for the Study of Reproduction
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424665001197568
score 15,811543

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