Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)

The genus Narcissus has several endemic, rare and/or threatened species in the Iberian Peninsula and North Africa. In vitro propagation is a useful tool for threatened plants conservation used in ex situ strategies. Thus, the aim of this work was to study the propagation in vitro of bulb scale expla...

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Detalles Bibliográficos
Autores: Juan Vicedo, Jorge, Pavlov, Atanas, Ríos, Segundo, Casas, José Luis
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/3255
Acceso en línea:http://hdl.handle.net/20.500.12466/3255
Access Level:acceso abierto
Palabra clave:Bulb scales
Conservation
Endangered plants
Micropropagation
Narcissus
Plant growth regulators
2417 Biología Vegetal (Botánica)
2417.19 Fisiología Vegetal
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spelling Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)Juan Vicedo, JorgePavlov, AtanasRíos, SegundoCasas, José LuisBulb scalesConservationEndangered plantsMicropropagationNarcissusPlant growth regulators2417 Biología Vegetal (Botánica)2417.19 Fisiología VegetalThe genus Narcissus has several endemic, rare and/or threatened species in the Iberian Peninsula and North Africa. In vitro propagation is a useful tool for threatened plants conservation used in ex situ strategies. Thus, the aim of this work was to study the propagation in vitro of bulb scale explants of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula, treated with different PGR combinations. Initiation was achieved in half-strength Murashige and Skoog (MS) basal salts and vitamins, 10 g/L sucrose, 500 mg/L casein hydrolysate, 2 mg/L adenine, 10 mg/L glutathione and 5.5 g/L plant agar. In the multiplication phase, the highest bulblet proliferation was obtained in MS medium supplemented with 30 g/L sucrose and the combination of 10 μM 6-Benzylaminopurine (BAP) + 5 μM α-Naphthaleneacetic acid (NAA) in N. alcaracensis, N. eugeniae and N. hedraeanthus; 20 μM BAP + 5 μM NAA in N. jonquilla and N. yepesii. The highest rooting was obtained with 5 μM NAA + 1 μM Indole-3-butyric acid (IBA) for all species (>75%) and more than 80% of the produced bulblets were successfully acclimatized.20232023-12-0520212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12466/3255reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártirinstname:Universidad Católica de Valencia San Vicente MártirInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riucv.ucv.es:20.500.12466/32552026-06-19T08:32:07Z
dc.title.none.fl_str_mv Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)
title Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)
spellingShingle Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)
Juan Vicedo, Jorge
Bulb scales
Conservation
Endangered plants
Micropropagation
Narcissus
Plant growth regulators
2417 Biología Vegetal (Botánica)
2417.19 Fisiología Vegetal
title_short Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)
title_full Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)
title_fullStr Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)
title_full_unstemmed Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)
title_sort Micropropagation of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula (Spain and Portugal)
dc.creator.none.fl_str_mv Juan Vicedo, Jorge
Pavlov, Atanas
Ríos, Segundo
Casas, José Luis
author Juan Vicedo, Jorge
author_facet Juan Vicedo, Jorge
Pavlov, Atanas
Ríos, Segundo
Casas, José Luis
author_role author
author2 Pavlov, Atanas
Ríos, Segundo
Casas, José Luis
author2_role author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Bulb scales
Conservation
Endangered plants
Micropropagation
Narcissus
Plant growth regulators
2417 Biología Vegetal (Botánica)
2417.19 Fisiología Vegetal
topic Bulb scales
Conservation
Endangered plants
Micropropagation
Narcissus
Plant growth regulators
2417 Biología Vegetal (Botánica)
2417.19 Fisiología Vegetal
description The genus Narcissus has several endemic, rare and/or threatened species in the Iberian Peninsula and North Africa. In vitro propagation is a useful tool for threatened plants conservation used in ex situ strategies. Thus, the aim of this work was to study the propagation in vitro of bulb scale explants of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula, treated with different PGR combinations. Initiation was achieved in half-strength Murashige and Skoog (MS) basal salts and vitamins, 10 g/L sucrose, 500 mg/L casein hydrolysate, 2 mg/L adenine, 10 mg/L glutathione and 5.5 g/L plant agar. In the multiplication phase, the highest bulblet proliferation was obtained in MS medium supplemented with 30 g/L sucrose and the combination of 10 μM 6-Benzylaminopurine (BAP) + 5 μM α-Naphthaleneacetic acid (NAA) in N. alcaracensis, N. eugeniae and N. hedraeanthus; 20 μM BAP + 5 μM NAA in N. jonquilla and N. yepesii. The highest rooting was obtained with 5 μM NAA + 1 μM Indole-3-butyric acid (IBA) for all species (>75%) and more than 80% of the produced bulblets were successfully acclimatized.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
2023
2023-12-05
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12466/3255
url http://hdl.handle.net/20.500.12466/3255
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
instname:Universidad Católica de Valencia San Vicente Mártir
instname_str Universidad Católica de Valencia San Vicente Mártir
reponame_str RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
collection RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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