A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study

Laser ablation ICP-mass spectrometry (LA-ICP-MS) has developed as a powerful tool for elemental quantitative analysis of individual cells, assuring that the content of each cell is analyzed individually. However, this technique is still limited by the difficulties associated with calibration using s...

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Autores: Bazo , Antonio, Bolea-Fernández , Eduardo, Billimoria , Kharmen, Rua-Ibarz , Ana, Aramendía , Maite, Menero-Valdés , Paula, Morley , Jack, Neves , Sara, Goenaga-Infante , Heidi, Resano , Martín, Sánchez Cachero, Armando
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/45648
Acceso en línea:https://doi.org/10.1039/D5JA00253B
https://hdl.handle.net/10578/45648
Access Level:acceso abierto
Palabra clave:Cancer cells
Gold Nanoparticles (AuNPs)
Laser Ablation ICP-MS
Particle mass calibration
Single-cell quantification
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spelling A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case studyBazo , AntonioBolea-Fernández , EduardoBillimoria , KharmenRua-Ibarz , AnaAramendía , MaiteMenero-Valdés , PaulaMorley , JackNeves , SaraGoenaga-Infante , HeidiResano , MartínSánchez Cachero, ArmandoCancer cellsGold Nanoparticles (AuNPs)Laser Ablation ICP-MSParticle mass calibrationSingle-cell quantificationLaser ablation ICP-mass spectrometry (LA-ICP-MS) has developed as a powerful tool for elemental quantitative analysis of individual cells, assuring that the content of each cell is analyzed individually. However, this technique is still limited by the difficulties associated with calibration using solid standards. This work proposes a particle mass calibration strategy that is independent of both the properties and thickness of the gelatin films used for calibration, overcoming a significant drawback of previously established methods. The fundamental principle of this strategy relies on the individual ablation of nanoparticles (NPs) of well-characterized size that are embedded in the films, so that their mass can be directly used for calibration without the need to calculate their exact concentration within the gelatin. The performance of the newly developed method was compared to that of the previously reported approaches (ionic and particle number calibration) in terms of linearity and homogeneity between different films prepared from the same gelatin solution. As a case study, the three calibration strategies were used for the quantitative analysis of HeLa cancer cells exposed to AuNPs. In parallel, in-suspension single-cell (SC) ICP-MS Au data were obtained and used as reference for comparison with the three LA-SC-ICP-MS strategies. The results obtained with the novel particle mass approach demonstrated better accuracy and repeatability over three different working sessions, addressing key limitations and providing a robust and reliable method for quantitative LA-SC-ICP-MS analysis. The particle mass method holds promise for quantitative LA-ICP-MS analysis of samples beyond NP-exposed cells, such as biological tissues.Royal Society of Chemistry202520252025info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://doi.org/10.1039/D5JA00253Bhttps://hdl.handle.net/10578/45648reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésPID2021-122455NB-I00RYC2021-031093-IMarie-Sklodowska-Curie n.º 101034288I3A ImpulsoDepartamento de Ciencia, Universidad y Sociedad del Conocimiento de la DGA su beca predoctoral (convocatoria de 2021)info:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/456482026-05-27T07:36:41Z
dc.title.none.fl_str_mv A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study
title A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study
spellingShingle A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study
Bazo , Antonio
Cancer cells
Gold Nanoparticles (AuNPs)
Laser Ablation ICP-MS
Particle mass calibration
Single-cell quantification
title_short A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study
title_full A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study
title_fullStr A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study
title_full_unstemmed A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study
title_sort A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study
dc.creator.none.fl_str_mv Bazo , Antonio
Bolea-Fernández , Eduardo
Billimoria , Kharmen
Rua-Ibarz , Ana
Aramendía , Maite
Menero-Valdés , Paula
Morley , Jack
Neves , Sara
Goenaga-Infante , Heidi
Resano , Martín
Sánchez Cachero, Armando
author Bazo , Antonio
author_facet Bazo , Antonio
Bolea-Fernández , Eduardo
Billimoria , Kharmen
Rua-Ibarz , Ana
Aramendía , Maite
Menero-Valdés , Paula
Morley , Jack
Neves , Sara
Goenaga-Infante , Heidi
Resano , Martín
Sánchez Cachero, Armando
author_role author
author2 Bolea-Fernández , Eduardo
Billimoria , Kharmen
Rua-Ibarz , Ana
Aramendía , Maite
Menero-Valdés , Paula
Morley , Jack
Neves , Sara
Goenaga-Infante , Heidi
Resano , Martín
Sánchez Cachero, Armando
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cancer cells
Gold Nanoparticles (AuNPs)
Laser Ablation ICP-MS
Particle mass calibration
Single-cell quantification
topic Cancer cells
Gold Nanoparticles (AuNPs)
Laser Ablation ICP-MS
Particle mass calibration
Single-cell quantification
description Laser ablation ICP-mass spectrometry (LA-ICP-MS) has developed as a powerful tool for elemental quantitative analysis of individual cells, assuring that the content of each cell is analyzed individually. However, this technique is still limited by the difficulties associated with calibration using solid standards. This work proposes a particle mass calibration strategy that is independent of both the properties and thickness of the gelatin films used for calibration, overcoming a significant drawback of previously established methods. The fundamental principle of this strategy relies on the individual ablation of nanoparticles (NPs) of well-characterized size that are embedded in the films, so that their mass can be directly used for calibration without the need to calculate their exact concentration within the gelatin. The performance of the newly developed method was compared to that of the previously reported approaches (ionic and particle number calibration) in terms of linearity and homogeneity between different films prepared from the same gelatin solution. As a case study, the three calibration strategies were used for the quantitative analysis of HeLa cancer cells exposed to AuNPs. In parallel, in-suspension single-cell (SC) ICP-MS Au data were obtained and used as reference for comparison with the three LA-SC-ICP-MS strategies. The results obtained with the novel particle mass approach demonstrated better accuracy and repeatability over three different working sessions, addressing key limitations and providing a robust and reliable method for quantitative LA-SC-ICP-MS analysis. The particle mass method holds promise for quantitative LA-ICP-MS analysis of samples beyond NP-exposed cells, such as biological tissues.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.1039/D5JA00253B
https://hdl.handle.net/10578/45648
url https://doi.org/10.1039/D5JA00253B
https://hdl.handle.net/10578/45648
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PID2021-122455NB-I00
RYC2021-031093-I
Marie-Sklodowska-Curie n.º 101034288
I3A Impulso
Departamento de Ciencia, Universidad y Sociedad del Conocimiento de la DGA su beca predoctoral (convocatoria de 2021)
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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