Improving separation optimization in capillary electrophoresis by using a general quality criterion

In this paper we extend the use of the quality criterion t' to optimize separations in capillary electrophoresis (CE). The theoretical parameter t' takes into account not only the relative separation between a given pair of compounds but also their separation from the neutral species migra...

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Detalles Bibliográficos
Autores: Peró Gascón, Roger, Tascon, Marcos, Sanz Nebot, María Victoria, Gagliardi, Riccardo, Benavente Moreno, Fernando J. (Julián)
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/151387
Acceso en línea:https://hdl.handle.net/2445/151387
Access Level:acceso abierto
Palabra clave:Electroforesi capil·lar
Concentració dels ions d'hidrogen
Capillary electrophoresis
Hydrogen-ion concentration
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spelling Improving separation optimization in capillary electrophoresis by using a general quality criterionPeró Gascón, RogerTascon, MarcosSanz Nebot, María VictoriaGagliardi, RiccardoBenavente Moreno, Fernando J. (Julián)Electroforesi capil·larConcentració dels ions d'hidrogenCapillary electrophoresisHydrogen-ion concentrationIn this paper we extend the use of the quality criterion t' to optimize separations in capillary electrophoresis (CE). The theoretical parameter t' takes into account not only the relative separation between a given pair of compounds but also their separation from the neutral species migrating with the electroosmotic flow (EOF). Furthermore, it can be composed for complex mixtures as a global multicriterium optimization function T', for a rapid, simple and reliable selection of optimized separation conditions by mathematical maximization. Here, we demonstrate the applicability of T' using as a variable the electrophoretic mobility (me) for the optimization of pH in the separation of a mixture of amyloid beta (Aβ) peptide fragments. In addition, it is shown the versatility of T' using other variables related to me, which do not require experimental measurements. This is the case with ionizable compounds as the Aβ peptide fragments, whose charge-to-mass ratios can be calculated if accurate pKa values are available in the literature. The excellent performance of T' for Aβ peptide fragments is further validated optimizing the pH for the separation of mixtures of harmala alkaloids (HAlks) and quinolone antibiotics.Elsevier B.V.2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/151387Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.talanta.2019.120399Talanta, 2020, vol. 208, p. 120399https://doi.org/10.1016/j.talanta.2019.120399cc-by-nc-nd (c) Elsevier B.V., 2020http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1513872026-05-27T06:46:51Z
dc.title.none.fl_str_mv Improving separation optimization in capillary electrophoresis by using a general quality criterion
title Improving separation optimization in capillary electrophoresis by using a general quality criterion
spellingShingle Improving separation optimization in capillary electrophoresis by using a general quality criterion
Peró Gascón, Roger
Electroforesi capil·lar
Concentració dels ions d'hidrogen
Capillary electrophoresis
Hydrogen-ion concentration
title_short Improving separation optimization in capillary electrophoresis by using a general quality criterion
title_full Improving separation optimization in capillary electrophoresis by using a general quality criterion
title_fullStr Improving separation optimization in capillary electrophoresis by using a general quality criterion
title_full_unstemmed Improving separation optimization in capillary electrophoresis by using a general quality criterion
title_sort Improving separation optimization in capillary electrophoresis by using a general quality criterion
dc.creator.none.fl_str_mv Peró Gascón, Roger
Tascon, Marcos
Sanz Nebot, María Victoria
Gagliardi, Riccardo
Benavente Moreno, Fernando J. (Julián)
author Peró Gascón, Roger
author_facet Peró Gascón, Roger
Tascon, Marcos
Sanz Nebot, María Victoria
Gagliardi, Riccardo
Benavente Moreno, Fernando J. (Julián)
author_role author
author2 Tascon, Marcos
Sanz Nebot, María Victoria
Gagliardi, Riccardo
Benavente Moreno, Fernando J. (Julián)
author2_role author
author
author
author
dc.subject.none.fl_str_mv Electroforesi capil·lar
Concentració dels ions d'hidrogen
Capillary electrophoresis
Hydrogen-ion concentration
topic Electroforesi capil·lar
Concentració dels ions d'hidrogen
Capillary electrophoresis
Hydrogen-ion concentration
description In this paper we extend the use of the quality criterion t' to optimize separations in capillary electrophoresis (CE). The theoretical parameter t' takes into account not only the relative separation between a given pair of compounds but also their separation from the neutral species migrating with the electroosmotic flow (EOF). Furthermore, it can be composed for complex mixtures as a global multicriterium optimization function T', for a rapid, simple and reliable selection of optimized separation conditions by mathematical maximization. Here, we demonstrate the applicability of T' using as a variable the electrophoretic mobility (me) for the optimization of pH in the separation of a mixture of amyloid beta (Aβ) peptide fragments. In addition, it is shown the versatility of T' using other variables related to me, which do not require experimental measurements. This is the case with ionizable compounds as the Aβ peptide fragments, whose charge-to-mass ratios can be calculated if accurate pKa values are available in the literature. The excellent performance of T' for Aβ peptide fragments is further validated optimizing the pH for the separation of mixtures of harmala alkaloids (HAlks) and quinolone antibiotics.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/151387
url https://hdl.handle.net/2445/151387
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.talanta.2019.120399
Talanta, 2020, vol. 208, p. 120399
https://doi.org/10.1016/j.talanta.2019.120399
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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