Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain

We aimed to obtain insights on the nature of a collection of isolates presumptively identified as atypical Streptococcus pneumoniae recovered from invasive and non-invasive infections in Spain. One-hundred and thirty-two isolates were characterized by: optochin susceptibility in ambient and CO(2)-en...

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Autores: Rolo, Dora, S Simões, Alexandra, Domenech, Arnau, Fenoll, Asuncion, Liñares, Josefina, de Lencastre, Hermínia, Ardanuy, Carmen, Sá-Leão, Raquel
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/6961
Acceso en línea:http://hdl.handle.net/20.500.12105/6961
Access Level:acceso abierto
Palabra clave:Female
Genotype
Humans
Male
Microbial Sensitivity Tests
Multilocus Sequence Typing
Phenotype
Phylogeny
Spain
Streptococcal Infections
Streptococcus
Streptococcus pneumoniae
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repository_id_str
spelling Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in SpainRolo, DoraS Simões, AlexandraDomenech, ArnauFenoll, AsuncionLiñares, Josefinade Lencastre, HermíniaArdanuy, CarmenSá-Leão, RaquelFemaleGenotypeHumansMaleMicrobial Sensitivity TestsMultilocus Sequence TypingPhenotypePhylogenySpainStreptococcal InfectionsStreptococcusStreptococcus pneumoniaeWe aimed to obtain insights on the nature of a collection of isolates presumptively identified as atypical Streptococcus pneumoniae recovered from invasive and non-invasive infections in Spain. One-hundred and thirty-two isolates were characterized by: optochin susceptibility in ambient and CO(2)-enriched atmosphere; bile solubility; PCR-based assays targeting pneumococcal genes lytA, ply, pspA, cpsA, Spn9802, aliB-like ORF2, and a specific 16S rRNA region; multilocus sequence analysis; and antimicrobial susceptibility. By multilocus sequence analysis, 61 isolates were S. pseudopneumoniae, 34 were pneumococci, 13 were S. mitis, and 24 remained unclassified as non-pneumococci. Among S. pseudopneumoniae isolates, 51 (83.6%) were collected from respiratory tract samples; eight isolates were obtained from sterile sources. High frequency of non-susceptibility to penicillin (60.7%) and erythromycin (42.6%) was found. Only 50.8% of the S. pseudopneumoniae isolates displayed the typical optochin phenotype originally described for this species. None harbored the cpsA gene or the pneumococcal typical lytA restriction fragment length polymorphism. The Spn9802 and the specific 16S rRNA regions were detected among the majority of the S. pseudopneumoniae isolates (n = 59 and n = 49, respectively). The ply and pspA genes were rarely found. A high genetic diversity was found and 59 profiles were identified. Among the S. pneumoniae, 23 were capsulated and 11 were non-typeable. Three non-typeable isolates, associated to international non-capsulated lineages, were recovered from invasive disease sources. In conclusion, half of the atypical pneumococcal clinical isolates were, in fact, S. pseudopneumoniae and one-fourth were other streptococci. We identified S. pseudopneumoniae and non-typeable pneumococci as cause of disease in Spain including invasive disease.Public Library of Science (PLOS)Ministerio de Ciencia e Innovación (España)Instituto de Salud Carlos IIIFundação para a Ciência e Tecnologia (Portugal)20182018-12-2720132013-02-2120132013-02-21journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/6961reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES PI060647 Not availableES PI090104 Not availableES BIO2008-02154 Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/69612026-06-12T12:43:37Z
dc.title.none.fl_str_mv Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain
title Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain
spellingShingle Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain
Rolo, Dora
Female
Genotype
Humans
Male
Microbial Sensitivity Tests
Multilocus Sequence Typing
Phenotype
Phylogeny
Spain
Streptococcal Infections
Streptococcus
Streptococcus pneumoniae
title_short Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain
title_full Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain
title_fullStr Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain
title_full_unstemmed Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain
title_sort Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain
dc.creator.none.fl_str_mv Rolo, Dora
S Simões, Alexandra
Domenech, Arnau
Fenoll, Asuncion
Liñares, Josefina
de Lencastre, Hermínia
Ardanuy, Carmen
Sá-Leão, Raquel
author Rolo, Dora
author_facet Rolo, Dora
S Simões, Alexandra
Domenech, Arnau
Fenoll, Asuncion
Liñares, Josefina
de Lencastre, Hermínia
Ardanuy, Carmen
Sá-Leão, Raquel
author_role author
author2 S Simões, Alexandra
Domenech, Arnau
Fenoll, Asuncion
Liñares, Josefina
de Lencastre, Hermínia
Ardanuy, Carmen
Sá-Leão, Raquel
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Instituto de Salud Carlos III
Fundação para a Ciência e Tecnologia (Portugal)
dc.subject.none.fl_str_mv Female
Genotype
Humans
Male
Microbial Sensitivity Tests
Multilocus Sequence Typing
Phenotype
Phylogeny
Spain
Streptococcal Infections
Streptococcus
Streptococcus pneumoniae
topic Female
Genotype
Humans
Male
Microbial Sensitivity Tests
Multilocus Sequence Typing
Phenotype
Phylogeny
Spain
Streptococcal Infections
Streptococcus
Streptococcus pneumoniae
description We aimed to obtain insights on the nature of a collection of isolates presumptively identified as atypical Streptococcus pneumoniae recovered from invasive and non-invasive infections in Spain. One-hundred and thirty-two isolates were characterized by: optochin susceptibility in ambient and CO(2)-enriched atmosphere; bile solubility; PCR-based assays targeting pneumococcal genes lytA, ply, pspA, cpsA, Spn9802, aliB-like ORF2, and a specific 16S rRNA region; multilocus sequence analysis; and antimicrobial susceptibility. By multilocus sequence analysis, 61 isolates were S. pseudopneumoniae, 34 were pneumococci, 13 were S. mitis, and 24 remained unclassified as non-pneumococci. Among S. pseudopneumoniae isolates, 51 (83.6%) were collected from respiratory tract samples; eight isolates were obtained from sterile sources. High frequency of non-susceptibility to penicillin (60.7%) and erythromycin (42.6%) was found. Only 50.8% of the S. pseudopneumoniae isolates displayed the typical optochin phenotype originally described for this species. None harbored the cpsA gene or the pneumococcal typical lytA restriction fragment length polymorphism. The Spn9802 and the specific 16S rRNA regions were detected among the majority of the S. pseudopneumoniae isolates (n = 59 and n = 49, respectively). The ply and pspA genes were rarely found. A high genetic diversity was found and 59 profiles were identified. Among the S. pneumoniae, 23 were capsulated and 11 were non-typeable. Three non-typeable isolates, associated to international non-capsulated lineages, were recovered from invasive disease sources. In conclusion, half of the atypical pneumococcal clinical isolates were, in fact, S. pseudopneumoniae and one-fourth were other streptococci. We identified S. pseudopneumoniae and non-typeable pneumococci as cause of disease in Spain including invasive disease.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-02-21
2013
2013-02-21
2018
2018-12-27
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/6961
url http://hdl.handle.net/20.500.12105/6961
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES PI060647 Not available
ES PI090104 Not available
ES BIO2008-02154 Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLOS)
publisher.none.fl_str_mv Public Library of Science (PLOS)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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