The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresis

6 p.-4 fig.

Detalhes bibliográficos
Autores: Schvartzman, Jorge Bernardo, Martínez-Robles, María Luisa, Hernandez, Pablo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:1993
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/210629
Acesso em linha:http://hdl.handle.net/10261/210629
Access Level:acceso abierto
Palavra-chave:Saccharomyces-cerevisiae
Potential origins
Egg extracts
Human-cells
Plasmids
Sequence
Type-1
Virus
Locus
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oai_identifier_str oai:digital.csic.es:10261/210629
network_acronym_str ES
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repository_id_str
spelling The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresisSchvartzman, Jorge BernardoMartínez-Robles, María LuisaHernandez, PabloSaccharomyces-cerevisiaePotential originsEgg extractsHuman-cellsPlasmidsSequenceType-1VirusLocus6 p.-4 fig.Initiation of DNA replication in higher eukaryotes is still a matter of controversy. Some evidence suggests it occurs at specific sites. Data obtained using two-dimensional (2D) agarose gel electrophoresis, however, led to the notion that it may occur at random in broad zones. This hypothesis is primarily based on the observation that several contiguous DNA fragments generate a mixture of the so-called 'bubble' and 'simple Y' patterns in Neutral/neutral 2D gels. The interpretation that this mixture of hybridisation patterns is indicative for random initiation of DNA synthesis relies on the assumption that replicative intermediates (RIs) containing an internal bubble where initiation occurred at different relative positions, generate comigrating signals. The latter, however, is still to be proven. We investigated this problem by analysing together, in the same 2D gel, populations of pBR322 RIs that were digested with different restriction endonucleases that cut the monomer only once at different locations. DNA synthesis begins at a specific site in pBR322 and progresses in a uni-directional manner. Thus, the main difference between these sets of RIs was the relative position of the origin. The results obtained clearly showed that populations of RIs containing an internal bubble where initiation occurred at different relative positions do not generate signals that co-migrate all-the-way in 2D gels. Despite this observation, however, our results support the notion that random initiation is indeed responsible for the peculiar 'bubble' signal observed in the case of several metazoan eukaryotes.This work was partially supported by grant 159/92 from the Comunidad Autónoma de Madrid, Spain and grant 93/0161 from the Spanish Fondo de Investigaciones Sanitarias.Peer reviewedOxford University PressComunidad de MadridInstituto de Salud Carlos IIISchvartzman, Jorge Bernardo [0000-0002-1856-6278]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020201993info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/210629reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.2903/j.efsa.2020.5991Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2106292026-05-22T06:33:51Z
dc.title.none.fl_str_mv The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresis
title The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresis
spellingShingle The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresis
Schvartzman, Jorge Bernardo
Saccharomyces-cerevisiae
Potential origins
Egg extracts
Human-cells
Plasmids
Sequence
Type-1
Virus
Locus
title_short The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresis
title_full The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresis
title_fullStr The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresis
title_full_unstemmed The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresis
title_sort The migration behaviour of DNA replicative intermediates containing an internal bubble analyzed by two-dimensional agarose gel electrophoresis
dc.creator.none.fl_str_mv Schvartzman, Jorge Bernardo
Martínez-Robles, María Luisa
Hernandez, Pablo
author Schvartzman, Jorge Bernardo
author_facet Schvartzman, Jorge Bernardo
Martínez-Robles, María Luisa
Hernandez, Pablo
author_role author
author2 Martínez-Robles, María Luisa
Hernandez, Pablo
author2_role author
author
dc.contributor.none.fl_str_mv Comunidad de Madrid
Instituto de Salud Carlos III
Schvartzman, Jorge Bernardo [0000-0002-1856-6278]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Saccharomyces-cerevisiae
Potential origins
Egg extracts
Human-cells
Plasmids
Sequence
Type-1
Virus
Locus
topic Saccharomyces-cerevisiae
Potential origins
Egg extracts
Human-cells
Plasmids
Sequence
Type-1
Virus
Locus
description 6 p.-4 fig.
publishDate 1993
dc.date.none.fl_str_mv 1993
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/210629
url http://hdl.handle.net/10261/210629
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.2903/j.efsa.2020.5991
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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