Long-term anaerobic conversion of lindane by electrochemically generated hydrogen

Lindane (γ‑HCH) persists in many aquifers where electron‑donor scarcity limits reductive dechlorination. Here we present a long‑term (116 d) microbial electrochemical technology (MET) that supplies cathodic H₂ to sustain lindane biotransformation. Sequentially decreasing the graphite‑brush cathode p...

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Detalhes bibliográficos
Autores: Wang, Guanxiong|||0009-0006-4859-7143, Fernandez Verdejo, David Juan|||0000-0002-3234-4006, Marco Urrea, Ernest|||0000-0002-8033-6553, Guisasola, Albert|||0000-0002-3012-7964, Blánquez Cano, Paqui|||0000-0003-2443-9977
Tipo de documento: artigo
Data de publicação:2026
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:dnet:uabarcelona_::06b46137fd08dabac6fd7477e733fc28
Acesso em linha:https://ddd.uab.cat/record/328872
https://dx.doi.org/urn:doi:10.1016/j.jhazmat.2026.142242
Access Level:Acceso aberto
Palavra-chave:Lindane
Microbial electrochemical technology
Reductive dechlorination
Hydrogen
Microbial community
Shewanella
Pseudomonas
Descrição
Resumo:Lindane (γ‑HCH) persists in many aquifers where electron‑donor scarcity limits reductive dechlorination. Here we present a long‑term (116 d) microbial electrochemical technology (MET) that supplies cathodic H₂ to sustain lindane biotransformation. Sequentially decreasing the graphite‑brush cathode potential from −0.6 to −0.7 and −0.8 V versus SHE raised headspace H₂ from < 0.1% to > 1% (v/v) and accelerated lindane degradation rates. Product inhibition led by the accumulation of monochlorobenzene and benzene stalled the culture. The application of three 15‑min N₂ flushing cycles restored activity each time and boosted the removal rate from 33.3 ± 1.2 (µM/d) to 85.3 ± 4.5 (µM/d) at −0.8 V. The value of the poised cathodic potential also affected the product distribution ratio of monochlorobenzene to benzene. Thus, the MET did not just provide a source of electrons but also seems to have an impact on the transformation pathway of lindane. 16S‑rRNA profiling revealed a biocathode dominated by Shewanella (14.6 ± 5.8%) and Pseudomonas (35.0 ± 8.2%), two genera potentially involved either in the direct transformation of lindane or in establishing an interspecies electron transfer network that enhances its reduction.