Rainbow trout integrated response after recovery from short-term acute hypoxia
Overcoming a stress situation, such as hypoxia episodes, which involve an allostatic load, will depend on the ability of fish to modulate physiological and biochemical systems to maintain homeostasis. The aim of the study was to determine the integrated stress response after acute hypoxia of rainbow...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/194302 |
| Acceso en línea: | https://hdl.handle.net/2445/194302 |
| Access Level: | acceso abierto |
| Palabra clave: | Anoxèmia Truites (Peixos) Estrès (Fisiologia) Expressió gènica Anoxemia Trout Stress (Physiology) Gene expression |
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Rainbow trout integrated response after recovery from short-term acute hypoxiaGarcía Meilán, IreneTort Bardolet, LluísKhansari, Ali RezaAnoxèmiaTruites (Peixos)Estrès (Fisiologia)Expressió gènicaAnoxemiaTroutStress (Physiology)Gene expressionOvercoming a stress situation, such as hypoxia episodes, which involve an allostatic load, will depend on the ability of fish to modulate physiological and biochemical systems to maintain homeostasis. The aim of the study was to determine the integrated stress response after acute hypoxia of rainbow trout considering the different elements and areas of the stress response: systemic and mucosal, local and global, from the systemic HPI axis to skin mucosa. For this purpose, trout were subjected to acute hypoxia (dissolved O2 down to 2 mg/L) for one hour and then recovered and sampled 1, 6 and 24h after reoxygenation. Physiological responses were significantly affected by hypoxic stress and their interaction with time after the challenge, being significant for plasma lactate and cortisol levels, in both plasma and skin mucus. At central brain level, only trh expression was modulated 1h after hypoxia which indicates that brain function is not heavily affected by this particular stress. Unlike brain, head kidney and skin were more affected by hypoxia and reoxygenation. In head kidney an upregulation in expression of most of the genes studied (gr, il1β, il6, tgfβ1, lysozyme, caspase 3, enolase, hif-1, myoglobin, sod2, gpx, gst and gsr) took place 6h after recovery, whereas only hsp70 and il10 were upregulated after 1h. On the contrary, in skin most of the analyzed genes showed a higher upregulation during 1h after stress suggesting that in the skin a local response took place as soon as the stressor was detected, thus indicating the importance of the skin in the building of a stress response, whereas the interrenal tissue participated in a later time point, to help preventing further alteration at central level. The present results also show that, even that the stressor is a physical/environmental stressor, all components of the biological systems participate in the regulation of the response and the recovery process, including neuroendocrine, metabolism and immunity.Frontiers Media2023202320222023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion14 p.application/pdfhttps://hdl.handle.net/2445/194302Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fphys.2022.1021927Frontiers in Physiology, 2022, vol. 13, num. 1021927https://doi.org/10.3389/fphys.2022.1021927cc-by (c) García Meilán, Irene et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1943022026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Rainbow trout integrated response after recovery from short-term acute hypoxia |
| title |
Rainbow trout integrated response after recovery from short-term acute hypoxia |
| spellingShingle |
Rainbow trout integrated response after recovery from short-term acute hypoxia García Meilán, Irene Anoxèmia Truites (Peixos) Estrès (Fisiologia) Expressió gènica Anoxemia Trout Stress (Physiology) Gene expression |
| title_short |
Rainbow trout integrated response after recovery from short-term acute hypoxia |
| title_full |
Rainbow trout integrated response after recovery from short-term acute hypoxia |
| title_fullStr |
Rainbow trout integrated response after recovery from short-term acute hypoxia |
| title_full_unstemmed |
Rainbow trout integrated response after recovery from short-term acute hypoxia |
| title_sort |
Rainbow trout integrated response after recovery from short-term acute hypoxia |
| dc.creator.none.fl_str_mv |
García Meilán, Irene Tort Bardolet, Lluís Khansari, Ali Reza |
| author |
García Meilán, Irene |
| author_facet |
García Meilán, Irene Tort Bardolet, Lluís Khansari, Ali Reza |
| author_role |
author |
| author2 |
Tort Bardolet, Lluís Khansari, Ali Reza |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Anoxèmia Truites (Peixos) Estrès (Fisiologia) Expressió gènica Anoxemia Trout Stress (Physiology) Gene expression |
| topic |
Anoxèmia Truites (Peixos) Estrès (Fisiologia) Expressió gènica Anoxemia Trout Stress (Physiology) Gene expression |
| description |
Overcoming a stress situation, such as hypoxia episodes, which involve an allostatic load, will depend on the ability of fish to modulate physiological and biochemical systems to maintain homeostasis. The aim of the study was to determine the integrated stress response after acute hypoxia of rainbow trout considering the different elements and areas of the stress response: systemic and mucosal, local and global, from the systemic HPI axis to skin mucosa. For this purpose, trout were subjected to acute hypoxia (dissolved O2 down to 2 mg/L) for one hour and then recovered and sampled 1, 6 and 24h after reoxygenation. Physiological responses were significantly affected by hypoxic stress and their interaction with time after the challenge, being significant for plasma lactate and cortisol levels, in both plasma and skin mucus. At central brain level, only trh expression was modulated 1h after hypoxia which indicates that brain function is not heavily affected by this particular stress. Unlike brain, head kidney and skin were more affected by hypoxia and reoxygenation. In head kidney an upregulation in expression of most of the genes studied (gr, il1β, il6, tgfβ1, lysozyme, caspase 3, enolase, hif-1, myoglobin, sod2, gpx, gst and gsr) took place 6h after recovery, whereas only hsp70 and il10 were upregulated after 1h. On the contrary, in skin most of the analyzed genes showed a higher upregulation during 1h after stress suggesting that in the skin a local response took place as soon as the stressor was detected, thus indicating the importance of the skin in the building of a stress response, whereas the interrenal tissue participated in a later time point, to help preventing further alteration at central level. The present results also show that, even that the stressor is a physical/environmental stressor, all components of the biological systems participate in the regulation of the response and the recovery process, including neuroendocrine, metabolism and immunity. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2023 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/194302 |
| url |
https://hdl.handle.net/2445/194302 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.3389/fphys.2022.1021927 Frontiers in Physiology, 2022, vol. 13, num. 1021927 https://doi.org/10.3389/fphys.2022.1021927 |
| dc.rights.none.fl_str_mv |
cc-by (c) García Meilán, Irene et al., 2022 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by (c) García Meilán, Irene et al., 2022 https://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
14 p. application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers Media |
| publisher.none.fl_str_mv |
Frontiers Media |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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