CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis

Background: The CX3CL1–CX3CR1 axis has been related to numerous diseases. The aim of our study was to investigate its involvement in coeliac disease (CD) pathogenesis, particularly in the early phase of the disease. Methods: We collected peripheral blood from CD patients and controls, enrolled in a...

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Autores: Fernández Prieto, Marta, Núñez, Concepción, Fernández Aceñero, María Jesús, López Palacios, Natalia, Bodas Pinedo, Andrés, Farrais, Sergio, Cuevas, David, Pascual, Virginia, Cerón Nieto, María Ángeles, Horta Herrera, Saúl, Espino Paisán, Laura, Salazar Mendoza, Isabel
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/12534
Acceso en línea:https://hdl.handle.net/20.500.14352/12534
Access Level:acceso abierto
Palabra clave:Coeliac disease
Fractalkine
CX3CL1
CX3CR1
Chemokines
Gastroenterología y hepatología
3205.03 Gastroenterología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/12534
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repository_id_str
spelling CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease PathogenesisFernández Prieto, MartaNúñez, ConcepciónFernández Aceñero, María JesúsLópez Palacios, NataliaBodas Pinedo, AndrésFarrais, SergioCuevas, DavidPascual, VirginiaCerón Nieto, María ÁngelesHorta Herrera, SaúlEspino Paisán, LauraSalazar Mendoza, IsabelCoeliac diseaseFractalkineCX3CL1CX3CR1ChemokinesGastroenterología y hepatología3205.03 GastroenterologíaBackground: The CX3CL1–CX3CR1 axis has been related to numerous diseases. The aim of our study was to investigate its involvement in coeliac disease (CD) pathogenesis, particularly in the early phase of the disease. Methods: We collected peripheral blood from CD patients and controls, enrolled in a 3-day gluten challenge, to study soluble CX3CL1, I-TAC and MIG by Luminex, CX3CL1 and CX3CR1 gene expression by qPCR, and CX3CR1 protein expression in monocytes and CD8+, CD4+ and γδ+ T cells, by flow cytometry. We also analysed the expression of the CX3CL1 and CX3CR1 mRNA and protein in the duodenal biopsies of CD patients with active and treated disease, and in non-CD control individuals, by qPCR and immunohistochemistry. Results: After the gluten challenge, increased levels of CX3CL1, I-TAC and MIG proteins were observed in the peripheral blood of CD patients, with no changes in CX3CL1 mRNA, or CX3CR1 mRNA and protein. Regarding duodenal tissue, CX3CL1 was absent or barely present in the superficial and basal epithelium of CD patients, contrasting with the moderate to high levels present in controls. Conclusions: CX3CL1 seems to be involved in the appearance and progression of CD, and it appears to be a potential diagnostic biomarker. Its use as an alternative therapeutic target in CD deserves further research.MDPIUniversidad Complutense de Madrid20192019-10-2320192019-10-23journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/12534reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/125342026-06-02T12:44:21Z
dc.title.none.fl_str_mv CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis
title CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis
spellingShingle CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis
Fernández Prieto, Marta
Coeliac disease
Fractalkine
CX3CL1
CX3CR1
Chemokines
Gastroenterología y hepatología
3205.03 Gastroenterología
title_short CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis
title_full CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis
title_fullStr CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis
title_full_unstemmed CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis
title_sort CX3CL1–CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis
dc.creator.none.fl_str_mv Fernández Prieto, Marta
Núñez, Concepción
Fernández Aceñero, María Jesús
López Palacios, Natalia
Bodas Pinedo, Andrés
Farrais, Sergio
Cuevas, David
Pascual, Virginia
Cerón Nieto, María Ángeles
Horta Herrera, Saúl
Espino Paisán, Laura
Salazar Mendoza, Isabel
author Fernández Prieto, Marta
author_facet Fernández Prieto, Marta
Núñez, Concepción
Fernández Aceñero, María Jesús
López Palacios, Natalia
Bodas Pinedo, Andrés
Farrais, Sergio
Cuevas, David
Pascual, Virginia
Cerón Nieto, María Ángeles
Horta Herrera, Saúl
Espino Paisán, Laura
Salazar Mendoza, Isabel
author_role author
author2 Núñez, Concepción
Fernández Aceñero, María Jesús
López Palacios, Natalia
Bodas Pinedo, Andrés
Farrais, Sergio
Cuevas, David
Pascual, Virginia
Cerón Nieto, María Ángeles
Horta Herrera, Saúl
Espino Paisán, Laura
Salazar Mendoza, Isabel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Coeliac disease
Fractalkine
CX3CL1
CX3CR1
Chemokines
Gastroenterología y hepatología
3205.03 Gastroenterología
topic Coeliac disease
Fractalkine
CX3CL1
CX3CR1
Chemokines
Gastroenterología y hepatología
3205.03 Gastroenterología
description Background: The CX3CL1–CX3CR1 axis has been related to numerous diseases. The aim of our study was to investigate its involvement in coeliac disease (CD) pathogenesis, particularly in the early phase of the disease. Methods: We collected peripheral blood from CD patients and controls, enrolled in a 3-day gluten challenge, to study soluble CX3CL1, I-TAC and MIG by Luminex, CX3CL1 and CX3CR1 gene expression by qPCR, and CX3CR1 protein expression in monocytes and CD8+, CD4+ and γδ+ T cells, by flow cytometry. We also analysed the expression of the CX3CL1 and CX3CR1 mRNA and protein in the duodenal biopsies of CD patients with active and treated disease, and in non-CD control individuals, by qPCR and immunohistochemistry. Results: After the gluten challenge, increased levels of CX3CL1, I-TAC and MIG proteins were observed in the peripheral blood of CD patients, with no changes in CX3CL1 mRNA, or CX3CR1 mRNA and protein. Regarding duodenal tissue, CX3CL1 was absent or barely present in the superficial and basal epithelium of CD patients, contrasting with the moderate to high levels present in controls. Conclusions: CX3CL1 seems to be involved in the appearance and progression of CD, and it appears to be a potential diagnostic biomarker. Its use as an alternative therapeutic target in CD deserves further research.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-10-23
2019
2019-10-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/12534
url https://hdl.handle.net/20.500.14352/12534
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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