The use of size exclusion chromatography to monitor protein self-assembly

This article belongs to the Special Issue Recent Advances in Protein Crystallography.

Detalles Bibliográficos
Autores: Adawy, Alaa, Groves, Matthew R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/360172
Acceso en línea:http://hdl.handle.net/10261/360172
Access Level:acceso abierto
Palabra clave:Size exclusion chromatography
Static light scattering
Protein aggregation
Protein nucleation
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spelling The use of size exclusion chromatography to monitor protein self-assemblyAdawy, AlaaGroves, Matthew R.Size exclusion chromatographyStatic light scatteringProtein aggregationProtein nucleationThis article belongs to the Special Issue Recent Advances in Protein Crystallography.High resolution size exclusion chromatography (SEC) coupled with static light scattering (SLS) analyses were conducted to study the effect of the mobile phase ionic strength and protein concentration on the output of SEC experiments. The results highlight the effect of small changes in the mobile phase composition on the estimation of molar masses estimated from retention time-based calibration curve compared with those obtained from SLS analysis. By comparing the SLS data with the SEC chromatograms, we show that SEC can provide helpful information on the protein aggregation state as macromolecules approach known precipitation points in their phase diagrams. This suggests the potential use of SEC as an easily accessible lab-based scanning methodology to monitor protein self-assembly prior to nucleation and crystallization. Implications for the use of SEC to study protein phase diagrams are discussed.We would like to thank Daiichi Sankyo Company Ltd. for funding this project through their TaNeDS program.Peer reviewedMultidisciplinary Digital Publishing InstituteDaiichi Sankyo Foundation of Life ScienceConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/360172reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/cryst7110331Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3601722026-05-22T06:33:51Z
dc.title.none.fl_str_mv The use of size exclusion chromatography to monitor protein self-assembly
title The use of size exclusion chromatography to monitor protein self-assembly
spellingShingle The use of size exclusion chromatography to monitor protein self-assembly
Adawy, Alaa
Size exclusion chromatography
Static light scattering
Protein aggregation
Protein nucleation
title_short The use of size exclusion chromatography to monitor protein self-assembly
title_full The use of size exclusion chromatography to monitor protein self-assembly
title_fullStr The use of size exclusion chromatography to monitor protein self-assembly
title_full_unstemmed The use of size exclusion chromatography to monitor protein self-assembly
title_sort The use of size exclusion chromatography to monitor protein self-assembly
dc.creator.none.fl_str_mv Adawy, Alaa
Groves, Matthew R.
author Adawy, Alaa
author_facet Adawy, Alaa
Groves, Matthew R.
author_role author
author2 Groves, Matthew R.
author2_role author
dc.contributor.none.fl_str_mv Daiichi Sankyo Foundation of Life Science
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Size exclusion chromatography
Static light scattering
Protein aggregation
Protein nucleation
topic Size exclusion chromatography
Static light scattering
Protein aggregation
Protein nucleation
description This article belongs to the Special Issue Recent Advances in Protein Crystallography.
publishDate 2017
dc.date.none.fl_str_mv 2017
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/360172
url http://hdl.handle.net/10261/360172
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/cryst7110331
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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