Relevance of the SlCIPK23 kinase in Na+ uptake and root morphology in K+-starved tomato plants

The beneficial effects of Na+ as a substitute for K+ have been well-documented at the physiological level. However, the transport systems and regulatory mechanisms that allow Na+ acquisition under K+ deficiency remain poorly understood in the majority of land plants. In tomato, SlCIPK23 kinase was i...

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Detalhes bibliográficos
Autores: Amo Pérez, Jesús, Martínez-Martínez, Almudena, Martínez, Vicente, Rubio, Francisco, Nieves-Cordones, Manuel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/367543
Acesso em linha:http://hdl.handle.net/10261/367543
https://api.elsevier.com/content/abstract/scopus_id/85183544049
Access Level:acceso abierto
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spelling Relevance of the SlCIPK23 kinase in Na+ uptake and root morphology in K+-starved tomato plantsAmo Pérez, JesúsMartínez-Martínez, AlmudenaMartínez, VicenteRubio, FranciscoNieves-Cordones, ManuelThe beneficial effects of Na+ as a substitute for K+ have been well-documented at the physiological level. However, the transport systems and regulatory mechanisms that allow Na+ acquisition under K+ deficiency remain poorly understood in the majority of land plants. In tomato, SlCIPK23 kinase was involved in Na+ accumulation in K+-starved plants, in addition to activating the LKT1 K+ channel and the K+ transporter SlHAK5. We used the central role of SlCIPK23 in K+ and Na+ acquisition to study which molecular entities mediate Na+ uptake with knockout tomato mutants and expression in heterologous systems. Two main pathways for Na+ uptake were deduced in tomato plants: an NH4+-sensitive pathway dependent on SlCIPK23, and a second one sensitive to Ba2+, Ca2+, La3+, and Li+. The addition of Na+ (10 mM) to lkt1, slhak5, or slcipk23 mutant KO lines produced interesting changes in root morphology. In particular, the roots of slcipk23 plants were longer and lighter than those of the WT under K+-deficient conditions and this effect was reversed by the addition of 10 mM Na+. These results provide a stimulating perspective for the study of the beneficial effects of Na+ in crops.This work was funded by grants 20806/PI/18 from Fundación Séneca (to FR), PID2019-106649RB-I00 from MICINN (to FR and MN-C), and Extraordinary grant preparation support 2023AEP051 from CSIC (to FR and MN-C). All grants from Spanish ministries were co-financed by the FEDER Fund. MN-C is the recipient of a Ramón y Cajal Fellowship (RyC-2017-21924) from MINECO, Spain. JA and AM-M are recipients of FPU predoctoral fellowships from the Ministerio de Educación (FPU2017-01883 and FPU20/04469, respectively). This work was also supported by the Red de Excelencia BIO2016-81957-REDT from MINECO. This study formed part of the AGROALNEXT program and was supported by MCIN with funding from European Union NextGenerationEU (PRTR-C17. I1) and by Fundación Séneca with funding from Comunidad Autónoma Región de Murcia (CARM)Peer reviewedElsevierFundación SénecaAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas (España)European CommissionMinisterio de Economía, Industria y Competitividad (España)Ministerio de Educación (España)Martínez, Vicente [0000-0002-3388-3116]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/367543https://api.elsevier.com/content/abstract/scopus_id/85183544049reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106649RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2017-21924info:eu-repo/grantAgreement//FPU2017-01883/info:eu-repo/grantAgreement//FPU20/04469Plant physiology and biochemistry : PPBThe underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.plaphy.2024.108373https://doi.org/10.1016/j.plaphy.2024.108373Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3675432026-05-22T06:33:51Z
dc.title.none.fl_str_mv Relevance of the SlCIPK23 kinase in Na+ uptake and root morphology in K+-starved tomato plants
title Relevance of the SlCIPK23 kinase in Na+ uptake and root morphology in K+-starved tomato plants
spellingShingle Relevance of the SlCIPK23 kinase in Na+ uptake and root morphology in K+-starved tomato plants
Amo Pérez, Jesús
title_short Relevance of the SlCIPK23 kinase in Na+ uptake and root morphology in K+-starved tomato plants
title_full Relevance of the SlCIPK23 kinase in Na+ uptake and root morphology in K+-starved tomato plants
title_fullStr Relevance of the SlCIPK23 kinase in Na+ uptake and root morphology in K+-starved tomato plants
title_full_unstemmed Relevance of the SlCIPK23 kinase in Na+ uptake and root morphology in K+-starved tomato plants
title_sort Relevance of the SlCIPK23 kinase in Na+ uptake and root morphology in K+-starved tomato plants
dc.creator.none.fl_str_mv Amo Pérez, Jesús
Martínez-Martínez, Almudena
Martínez, Vicente
Rubio, Francisco
Nieves-Cordones, Manuel
author Amo Pérez, Jesús
author_facet Amo Pérez, Jesús
Martínez-Martínez, Almudena
Martínez, Vicente
Rubio, Francisco
Nieves-Cordones, Manuel
author_role author
author2 Martínez-Martínez, Almudena
Martínez, Vicente
Rubio, Francisco
Nieves-Cordones, Manuel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Fundación Séneca
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas (España)
European Commission
Ministerio de Economía, Industria y Competitividad (España)
Ministerio de Educación (España)
Martínez, Vicente [0000-0002-3388-3116]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description The beneficial effects of Na+ as a substitute for K+ have been well-documented at the physiological level. However, the transport systems and regulatory mechanisms that allow Na+ acquisition under K+ deficiency remain poorly understood in the majority of land plants. In tomato, SlCIPK23 kinase was involved in Na+ accumulation in K+-starved plants, in addition to activating the LKT1 K+ channel and the K+ transporter SlHAK5. We used the central role of SlCIPK23 in K+ and Na+ acquisition to study which molecular entities mediate Na+ uptake with knockout tomato mutants and expression in heterologous systems. Two main pathways for Na+ uptake were deduced in tomato plants: an NH4+-sensitive pathway dependent on SlCIPK23, and a second one sensitive to Ba2+, Ca2+, La3+, and Li+. The addition of Na+ (10 mM) to lkt1, slhak5, or slcipk23 mutant KO lines produced interesting changes in root morphology. In particular, the roots of slcipk23 plants were longer and lighter than those of the WT under K+-deficient conditions and this effect was reversed by the addition of 10 mM Na+. These results provide a stimulating perspective for the study of the beneficial effects of Na+ in crops.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/367543
https://api.elsevier.com/content/abstract/scopus_id/85183544049
url http://hdl.handle.net/10261/367543
https://api.elsevier.com/content/abstract/scopus_id/85183544049
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106649RB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2017-21924
info:eu-repo/grantAgreement//FPU2017-01883/
info:eu-repo/grantAgreement//FPU20/04469
Plant physiology and biochemistry : PPB
The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.plaphy.2024.108373
https://doi.org/10.1016/j.plaphy.2024.108373
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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