Interactive effects of warming, antibiotics, and nanoplastics on the gut microbiome of the collembolan Folsomia candida

At 20 °C antibiotic exposure led to a loss of gut microbiome evenness. - Gram-negative bacteria targeted by colistin were not globally affected. - At 20 °C nanoplastic exposure reduced relative abundance of Actinobacteria and Firmicutes. - Wolbachia genus controlled compositional shifts under nanopl...

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Autores: Ferrín Guardiola, Miquel|||0000-0001-5030-094X, Marquez, Laura, Domene, Xavier|||0000-0002-2951-1491, Zhu, Dong|||0000-0002-0826-6423, Zhu, Yong-Guan|||0000-0003-3861-8482, Peñuelas, Josep|||0000-0002-7215-0150, Peguero, Guille|||0000-0002-6464-1486
Tipo de documento: artigo
Data de publicação:2025
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:312786
Acesso em linha:https://ddd.uab.cat/record/312786
https://dx.doi.org/urn:doi:10.1007/s42832-024-0269-8
Access Level:Acceso aberto
Palavra-chave:Xenobiotics
Bacteria
Colistin
Microplastics
Folsomia candida
Global change
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spelling Interactive effects of warming, antibiotics, and nanoplastics on the gut microbiome of the collembolan Folsomia candidaFerrín Guardiola, Miquel|||0000-0001-5030-094XMarquez, LauraDomene, Xavier|||0000-0002-2951-1491Zhu, Dong|||0000-0002-0826-6423Zhu, Yong-Guan|||0000-0003-3861-8482Peñuelas, Josep|||0000-0002-7215-0150Peguero, Guille|||0000-0002-6464-1486XenobioticsBacteriaColistinMicroplasticsFolsomia candidaGlobal changeAt 20 °C antibiotic exposure led to a loss of gut microbiome evenness. - Gram-negative bacteria targeted by colistin were not globally affected. - At 20 °C nanoplastic exposure reduced relative abundance of Actinobacteria and Firmicutes. - Wolbachia genus controlled compositional shifts under nanoplastic addition. - At 22 °C nanoplastic exposure reduced abundance, increased evenness, and changed gut microbiome composition. - Nanoplastics and antibiotics are among the most abundant chemical pollutants of soils, but their interplay with global warming remains poorly understood. The springtail Folsomia candida (Class Collembola) is a standard model for ecotoxicological assays with potential as a bioindicator of xenobiotics. Little is known, however, about their gut microbiome and how it might respond to warming and these pollutants. We exposed populations of F. candida to nanoplastics and antibiotic under two temperatures. The antibiotic treatment consisted of colistin addition, and the nanoplastic treatment consisted of polystyrene particles (50 mg kg-1 and 0.1 g kg-1 of dry soil, respectively). Both treatments were incubated at 20 and 22 °C for two months, and the bacterial gut microbiomes of springtails were then sequenced. Exposure to nanoplastics at 20 °C decreased the abundance of the dominant bacterial phyla and families, and decreased the evenness of the gut microbiome. At 22 °C, however, the abundances and evenness of the dominant families increased. Surprisingly, Gramnegative bacteria targeted by colistin were not globally affected. And at genus-level, the endosymbiont Wolbachia controlled the compositional shifts under nanoplastic addition, potentially driving the gut microbiome. Our results also indicated that warming was a major driver modulating the impacts of the antibiotic and nanoplastics. We illustrate how the gut microbiomes of springtails are sensitive communities responsive to xenobiotics and provide evidence of the need to combine multiple factors of global change operating simultaneously if we are to understand the responses of communities of soil arthropods and their microbiomes. 22025-01-0120252025-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/312786https://dx.doi.org/urn:doi:10.1007/s42832-024-0269-8reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2022-140808NB-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 TED2021-132627B-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-115770RB-I00Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-1333open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3127862026-06-06T12:50:31Z
dc.title.none.fl_str_mv Interactive effects of warming, antibiotics, and nanoplastics on the gut microbiome of the collembolan Folsomia candida
title Interactive effects of warming, antibiotics, and nanoplastics on the gut microbiome of the collembolan Folsomia candida
spellingShingle Interactive effects of warming, antibiotics, and nanoplastics on the gut microbiome of the collembolan Folsomia candida
Ferrín Guardiola, Miquel|||0000-0001-5030-094X
Xenobiotics
Bacteria
Colistin
Microplastics
Folsomia candida
Global change
title_short Interactive effects of warming, antibiotics, and nanoplastics on the gut microbiome of the collembolan Folsomia candida
title_full Interactive effects of warming, antibiotics, and nanoplastics on the gut microbiome of the collembolan Folsomia candida
title_fullStr Interactive effects of warming, antibiotics, and nanoplastics on the gut microbiome of the collembolan Folsomia candida
title_full_unstemmed Interactive effects of warming, antibiotics, and nanoplastics on the gut microbiome of the collembolan Folsomia candida
title_sort Interactive effects of warming, antibiotics, and nanoplastics on the gut microbiome of the collembolan Folsomia candida
dc.creator.none.fl_str_mv Ferrín Guardiola, Miquel|||0000-0001-5030-094X
Marquez, Laura
Domene, Xavier|||0000-0002-2951-1491
Zhu, Dong|||0000-0002-0826-6423
Zhu, Yong-Guan|||0000-0003-3861-8482
Peñuelas, Josep|||0000-0002-7215-0150
Peguero, Guille|||0000-0002-6464-1486
author Ferrín Guardiola, Miquel|||0000-0001-5030-094X
author_facet Ferrín Guardiola, Miquel|||0000-0001-5030-094X
Marquez, Laura
Domene, Xavier|||0000-0002-2951-1491
Zhu, Dong|||0000-0002-0826-6423
Zhu, Yong-Guan|||0000-0003-3861-8482
Peñuelas, Josep|||0000-0002-7215-0150
Peguero, Guille|||0000-0002-6464-1486
author_role author
author2 Marquez, Laura
Domene, Xavier|||0000-0002-2951-1491
Zhu, Dong|||0000-0002-0826-6423
Zhu, Yong-Guan|||0000-0003-3861-8482
Peñuelas, Josep|||0000-0002-7215-0150
Peguero, Guille|||0000-0002-6464-1486
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Xenobiotics
Bacteria
Colistin
Microplastics
Folsomia candida
Global change
topic Xenobiotics
Bacteria
Colistin
Microplastics
Folsomia candida
Global change
description At 20 °C antibiotic exposure led to a loss of gut microbiome evenness. - Gram-negative bacteria targeted by colistin were not globally affected. - At 20 °C nanoplastic exposure reduced relative abundance of Actinobacteria and Firmicutes. - Wolbachia genus controlled compositional shifts under nanoplastic addition. - At 22 °C nanoplastic exposure reduced abundance, increased evenness, and changed gut microbiome composition. - Nanoplastics and antibiotics are among the most abundant chemical pollutants of soils, but their interplay with global warming remains poorly understood. The springtail Folsomia candida (Class Collembola) is a standard model for ecotoxicological assays with potential as a bioindicator of xenobiotics. Little is known, however, about their gut microbiome and how it might respond to warming and these pollutants. We exposed populations of F. candida to nanoplastics and antibiotic under two temperatures. The antibiotic treatment consisted of colistin addition, and the nanoplastic treatment consisted of polystyrene particles (50 mg kg-1 and 0.1 g kg-1 of dry soil, respectively). Both treatments were incubated at 20 and 22 °C for two months, and the bacterial gut microbiomes of springtails were then sequenced. Exposure to nanoplastics at 20 °C decreased the abundance of the dominant bacterial phyla and families, and decreased the evenness of the gut microbiome. At 22 °C, however, the abundances and evenness of the dominant families increased. Surprisingly, Gramnegative bacteria targeted by colistin were not globally affected. And at genus-level, the endosymbiont Wolbachia controlled the compositional shifts under nanoplastic addition, potentially driving the gut microbiome. Our results also indicated that warming was a major driver modulating the impacts of the antibiotic and nanoplastics. We illustrate how the gut microbiomes of springtails are sensitive communities responsive to xenobiotics and provide evidence of the need to combine multiple factors of global change operating simultaneously if we are to understand the responses of communities of soil arthropods and their microbiomes.
publishDate 2025
dc.date.none.fl_str_mv 2
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/312786
https://dx.doi.org/urn:doi:10.1007/s42832-024-0269-8
url https://ddd.uab.cat/record/312786
https://dx.doi.org/urn:doi:10.1007/s42832-024-0269-8
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2022-140808NB-I00
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 TED2021-132627B-I00
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-115770RB-I00
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-1333
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
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