Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor I
Previous results have shown that in rats with non-ascitic cirrhosis there is an altered transport of sugars and amino acids associated with elongated microvilli. These alterations returned to normal with the administration of Insulin-Like Growth Factor-I (IGF-I). The aims of this study were to explo...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2004 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/27575 |
| Acceso en línea: | https://hdl.handle.net/10171/27575 |
| Access Level: | acceso abierto |
| Palabra clave: | Galactose/pharmacokinetics Insulin-Like Growth Factor I/metabolism Liver Cirrhosis/chemically induced/metabolism/pathology Anatomía patológica |
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Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor ICastilla-Cortazar, I. (Inma)|||/items/65b2c98e-c765-4a59-a1e5-938b3301b9abPascual, M. (María)|||/items/79edf488-c054-4b6b-96e8-2caee2a58b22Urdaneta, E. (Elena)|||/items/3a1485af-544d-417f-b20d-b4bf98fdb182Pardo-Mindan, F.J. (Francisco Javier)|||/items/dda37df0-e0d1-4127-a3a2-b70505381a3fPuche, J.E. (Juan Enrique)|||/items/7a0b037b-b50a-4d6b-9eb5-b0b929a82799Vivas, B. (Bárbara)|||/items/92996bf5-ba77-419a-876f-75bc95a05505Diaz-Casares, A. (Amalia)|||/items/96f876f3-bf60-41f7-b3f2-1257fbe72f26García-Fernández, M.I. (María Inmaculada)|||/items/c125315c-27d1-4911-9c3f-ee2291d44cd8Diaz-Sanchez, M. (Matías)|||/items/f4f0cc9b-6709-4350-b37a-9e11e1fa4225Varela-Nieto, I. (Isabel)|||/items/58026668-3aec-47c3-aa31-d111962dd53dCastilla, A. (Alberto)|||/items/8e4bc780-6d3c-4b19-a964-ea70c06ae929Gonzalez-Baron, S. (Salvador)|||/items/1a048734-00bc-44e0-84b8-e1a579105f1eGalactose/pharmacokineticsInsulin-Like Growth Factor I/metabolismLiver Cirrhosis/chemically induced/metabolism/pathologyAnatomía patológicaPrevious results have shown that in rats with non-ascitic cirrhosis there is an altered transport of sugars and amino acids associated with elongated microvilli. These alterations returned to normal with the administration of Insulin-Like Growth Factor-I (IGF-I). The aims of this study were to explore the evolution of these alterations and analyse the effect of IGF-I in rats with advanced cirrhosis and ascites. Thus, jejunal structure and nutrient transport (D-galactose, L-leucine, L-proline, L-glutamic acid and L-cystine) were studied in rats with ascitic cirrhosis. METHODS: Advanced cirrhosis was induced by CCl4 inhalation and Phenobarbital administration for 30 weeks. Cirrhotic animals were divided into two groups which received IGF-I or saline during two weeks. Control group was studied in parallel. Jejunal microvilli were studied by electron microscopy. Nutrient transport was assessed in brush border membrane vesicles using 14C or 35S-labelled subtracts in the three experimental groups. RESULTS: Intestinal active Na+-dependent transport was significantly reduced in untreated cirrhotic rats. Kinetic studies showed a decreased Vmax and a reduced affinity for sugar and four amino acids transporters (expressed as an increased Kt) in the brush border membrane vesicles from untreated cirrhotic rats as compared with controls. Both parameters were normalised in the IGF-I-treated cirrhotic group. Electron microscopy showed elongation and fusion of microvilli with degenerative membrane lesions and/or notable atrophy. CONCLUSIONS: The initial microvilli elongation reported in non ascitic cirrhosis develops into atrophy in rats with advanced cirrhosis and nutrient transports (monosaccharides and amino acids) are progressively reduced. Both morphological and functional alterations improved significantly with low doses of IGF-I.BioMed CentralDadun. Depósito Académico Digital Universidad de Navarra20132013-01-0820042004-01-0120042004-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/27575reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/275752026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor I |
| title |
Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor I |
| spellingShingle |
Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor I Castilla-Cortazar, I. (Inma)|||/items/65b2c98e-c765-4a59-a1e5-938b3301b9ab Galactose/pharmacokinetics Insulin-Like Growth Factor I/metabolism Liver Cirrhosis/chemically induced/metabolism/pathology Anatomía patológica |
| title_short |
Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor I |
| title_full |
Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor I |
| title_fullStr |
Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor I |
| title_full_unstemmed |
Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor I |
| title_sort |
Jejunal microvilli atrophy and reduced nutrient transport in rats with advanced liver cirrhosis: improvement by Insulin-like Growth Factor I |
| dc.creator.none.fl_str_mv |
Castilla-Cortazar, I. (Inma)|||/items/65b2c98e-c765-4a59-a1e5-938b3301b9ab Pascual, M. (María)|||/items/79edf488-c054-4b6b-96e8-2caee2a58b22 Urdaneta, E. (Elena)|||/items/3a1485af-544d-417f-b20d-b4bf98fdb182 Pardo-Mindan, F.J. (Francisco Javier)|||/items/dda37df0-e0d1-4127-a3a2-b70505381a3f Puche, J.E. (Juan Enrique)|||/items/7a0b037b-b50a-4d6b-9eb5-b0b929a82799 Vivas, B. (Bárbara)|||/items/92996bf5-ba77-419a-876f-75bc95a05505 Diaz-Casares, A. (Amalia)|||/items/96f876f3-bf60-41f7-b3f2-1257fbe72f26 García-Fernández, M.I. (María Inmaculada)|||/items/c125315c-27d1-4911-9c3f-ee2291d44cd8 Diaz-Sanchez, M. (Matías)|||/items/f4f0cc9b-6709-4350-b37a-9e11e1fa4225 Varela-Nieto, I. (Isabel)|||/items/58026668-3aec-47c3-aa31-d111962dd53d Castilla, A. (Alberto)|||/items/8e4bc780-6d3c-4b19-a964-ea70c06ae929 Gonzalez-Baron, S. (Salvador)|||/items/1a048734-00bc-44e0-84b8-e1a579105f1e |
| author |
Castilla-Cortazar, I. (Inma)|||/items/65b2c98e-c765-4a59-a1e5-938b3301b9ab |
| author_facet |
Castilla-Cortazar, I. (Inma)|||/items/65b2c98e-c765-4a59-a1e5-938b3301b9ab Pascual, M. (María)|||/items/79edf488-c054-4b6b-96e8-2caee2a58b22 Urdaneta, E. (Elena)|||/items/3a1485af-544d-417f-b20d-b4bf98fdb182 Pardo-Mindan, F.J. (Francisco Javier)|||/items/dda37df0-e0d1-4127-a3a2-b70505381a3f Puche, J.E. (Juan Enrique)|||/items/7a0b037b-b50a-4d6b-9eb5-b0b929a82799 Vivas, B. (Bárbara)|||/items/92996bf5-ba77-419a-876f-75bc95a05505 Diaz-Casares, A. (Amalia)|||/items/96f876f3-bf60-41f7-b3f2-1257fbe72f26 García-Fernández, M.I. (María Inmaculada)|||/items/c125315c-27d1-4911-9c3f-ee2291d44cd8 Diaz-Sanchez, M. (Matías)|||/items/f4f0cc9b-6709-4350-b37a-9e11e1fa4225 Varela-Nieto, I. (Isabel)|||/items/58026668-3aec-47c3-aa31-d111962dd53d Castilla, A. (Alberto)|||/items/8e4bc780-6d3c-4b19-a964-ea70c06ae929 Gonzalez-Baron, S. (Salvador)|||/items/1a048734-00bc-44e0-84b8-e1a579105f1e |
| author_role |
author |
| author2 |
Pascual, M. (María)|||/items/79edf488-c054-4b6b-96e8-2caee2a58b22 Urdaneta, E. (Elena)|||/items/3a1485af-544d-417f-b20d-b4bf98fdb182 Pardo-Mindan, F.J. (Francisco Javier)|||/items/dda37df0-e0d1-4127-a3a2-b70505381a3f Puche, J.E. (Juan Enrique)|||/items/7a0b037b-b50a-4d6b-9eb5-b0b929a82799 Vivas, B. (Bárbara)|||/items/92996bf5-ba77-419a-876f-75bc95a05505 Diaz-Casares, A. (Amalia)|||/items/96f876f3-bf60-41f7-b3f2-1257fbe72f26 García-Fernández, M.I. (María Inmaculada)|||/items/c125315c-27d1-4911-9c3f-ee2291d44cd8 Diaz-Sanchez, M. (Matías)|||/items/f4f0cc9b-6709-4350-b37a-9e11e1fa4225 Varela-Nieto, I. (Isabel)|||/items/58026668-3aec-47c3-aa31-d111962dd53d Castilla, A. (Alberto)|||/items/8e4bc780-6d3c-4b19-a964-ea70c06ae929 Gonzalez-Baron, S. (Salvador)|||/items/1a048734-00bc-44e0-84b8-e1a579105f1e |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Galactose/pharmacokinetics Insulin-Like Growth Factor I/metabolism Liver Cirrhosis/chemically induced/metabolism/pathology Anatomía patológica |
| topic |
Galactose/pharmacokinetics Insulin-Like Growth Factor I/metabolism Liver Cirrhosis/chemically induced/metabolism/pathology Anatomía patológica |
| description |
Previous results have shown that in rats with non-ascitic cirrhosis there is an altered transport of sugars and amino acids associated with elongated microvilli. These alterations returned to normal with the administration of Insulin-Like Growth Factor-I (IGF-I). The aims of this study were to explore the evolution of these alterations and analyse the effect of IGF-I in rats with advanced cirrhosis and ascites. Thus, jejunal structure and nutrient transport (D-galactose, L-leucine, L-proline, L-glutamic acid and L-cystine) were studied in rats with ascitic cirrhosis. METHODS: Advanced cirrhosis was induced by CCl4 inhalation and Phenobarbital administration for 30 weeks. Cirrhotic animals were divided into two groups which received IGF-I or saline during two weeks. Control group was studied in parallel. Jejunal microvilli were studied by electron microscopy. Nutrient transport was assessed in brush border membrane vesicles using 14C or 35S-labelled subtracts in the three experimental groups. RESULTS: Intestinal active Na+-dependent transport was significantly reduced in untreated cirrhotic rats. Kinetic studies showed a decreased Vmax and a reduced affinity for sugar and four amino acids transporters (expressed as an increased Kt) in the brush border membrane vesicles from untreated cirrhotic rats as compared with controls. Both parameters were normalised in the IGF-I-treated cirrhotic group. Electron microscopy showed elongation and fusion of microvilli with degenerative membrane lesions and/or notable atrophy. CONCLUSIONS: The initial microvilli elongation reported in non ascitic cirrhosis develops into atrophy in rats with advanced cirrhosis and nutrient transports (monosaccharides and amino acids) are progressively reduced. Both morphological and functional alterations improved significantly with low doses of IGF-I. |
| publishDate |
2004 |
| dc.date.none.fl_str_mv |
2004 2004-01-01 2004 2004-01-01 2013 2013-01-08 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/27575 |
| url |
https://hdl.handle.net/10171/27575 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
BioMed Central |
| publisher.none.fl_str_mv |
BioMed Central |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869424518338969600 |
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15.301603 |