Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated with Clove Oil and MS-222: Effects on stress axis regulation and intermediary metabolism

Transport processes between aquaculture facilities activate the stress response in fish. To deal with these situations, the hypothalamic-pituitary-interrenal (HPI) axis releases cortisol, leading to an increase in circulating energy resources to restore homeostasis. However, if the allostatic load g...

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Authors: Jerez-Cepa, Ismael, Fernández-Castro, Miriam, Santo O'Neill, Thomas-Julian del, Martos-Sitcha, Juan Antonio, Martínez-Rodríguez, Gonzalo, Mancera, Juan Miguel, Ruiz-Jarabo, Ignacio
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/209690
Online Access:http://hdl.handle.net/10261/209690
Access Level:Open access
Keyword:welfare
Sparus aurata
Anesthetics
transport
clove oil
MS-222
Stress
intermediary metabolism
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oai_identifier_str oai:digital.csic.es:10261/209690
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated with Clove Oil and MS-222: Effects on stress axis regulation and intermediary metabolism
title Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated with Clove Oil and MS-222: Effects on stress axis regulation and intermediary metabolism
spellingShingle Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated with Clove Oil and MS-222: Effects on stress axis regulation and intermediary metabolism
Jerez-Cepa, Ismael
welfare
Sparus aurata
Anesthetics
transport
clove oil
MS-222
Stress
intermediary metabolism
title_short Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated with Clove Oil and MS-222: Effects on stress axis regulation and intermediary metabolism
title_full Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated with Clove Oil and MS-222: Effects on stress axis regulation and intermediary metabolism
title_fullStr Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated with Clove Oil and MS-222: Effects on stress axis regulation and intermediary metabolism
title_full_unstemmed Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated with Clove Oil and MS-222: Effects on stress axis regulation and intermediary metabolism
title_sort Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated with Clove Oil and MS-222: Effects on stress axis regulation and intermediary metabolism
dc.creator.none.fl_str_mv Jerez-Cepa, Ismael
Fernández-Castro, Miriam
Santo O'Neill, Thomas-Julian del
Martos-Sitcha, Juan Antonio
Martínez-Rodríguez, Gonzalo
Mancera, Juan Miguel
Ruiz-Jarabo, Ignacio
author Jerez-Cepa, Ismael
author_facet Jerez-Cepa, Ismael
Fernández-Castro, Miriam
Santo O'Neill, Thomas-Julian del
Martos-Sitcha, Juan Antonio
Martínez-Rodríguez, Gonzalo
Mancera, Juan Miguel
Ruiz-Jarabo, Ignacio
author_role author
author2 Fernández-Castro, Miriam
Santo O'Neill, Thomas-Julian del
Martos-Sitcha, Juan Antonio
Martínez-Rodríguez, Gonzalo
Mancera, Juan Miguel
Ruiz-Jarabo, Ignacio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Universidad de Cádiz
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv welfare
Sparus aurata
Anesthetics
transport
clove oil
MS-222
Stress
intermediary metabolism
topic welfare
Sparus aurata
Anesthetics
transport
clove oil
MS-222
Stress
intermediary metabolism
description Transport processes between aquaculture facilities activate the stress response in fish. To deal with these situations, the hypothalamic-pituitary-interrenal (HPI) axis releases cortisol, leading to an increase in circulating energy resources to restore homeostasis. However, if the allostatic load generated exceeds fish tolerance limits, stress-related responses will compromise health and welfare of the animals. In this context, anesthetics have arisen as potential agents aiming to reduce negative effects of stress response. Here we assessed the effects of a sedative dose of clove oil (CO) and MS-222 on hallmarks involved in HPI axis regulation and energy management after simulated transport, and further recovery, in gilthead seabream (Sparus aurata L.) juveniles. Fish were placed in a mobile setup of water tanks where transport conditions were simulated for 6 h. Sedation doses of either CO (2.5 mg L−1) or MS-222 (5 mg L−1) were added in the water tanks. A control group without anesthetics was also included in the setup. Half of the animals (n = 12 per group) were sampled immediately after transport, while remaining animals were allowed to recover for 18 h in clean water tanks and then sampled. Our results showed that the HPI axis response was modified at peripheral level, with differences depending on the anesthetic employed. Head kidney gene-expressions related to cortisol production (star and cyp11b1) matched concomitantly with increased plasma cortisol levels immediately after transport in CO-sedated fish, but these levels remained constant in MS-222-sedated fish. Differential changes in the energy management of carbohydrates, lipids and amino acids, depending on the anesthetic employed, were also observed. The use of CO stimulated amino acids catabolism, while MS-222-sedated fish tended to consume liver glycogen and mobilize triglycerides. Further studies, including alternative doses of both anestethics, as well as the assessment of time-course HPI activation and longer recovery periods, are necessary to better understand if the use of clove oil and MS-222 is beneficial for S. aurata under these circumstances.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/209690
url http://hdl.handle.net/10261/209690
dc.language.none.fl_str_mv Italiano
language_invalid_str_mv Italiano
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info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-76069-C2-1-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-81808-REDT
http://doi.org/10.3389/fphys.2019.00612
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated with Clove Oil and MS-222: Effects on stress axis regulation and intermediary metabolismJerez-Cepa, IsmaelFernández-Castro, MiriamSanto O'Neill, Thomas-Julian delMartos-Sitcha, Juan AntonioMartínez-Rodríguez, GonzaloMancera, Juan MiguelRuiz-Jarabo, IgnaciowelfareSparus aurataAnestheticstransportclove oilMS-222Stressintermediary metabolismTransport processes between aquaculture facilities activate the stress response in fish. To deal with these situations, the hypothalamic-pituitary-interrenal (HPI) axis releases cortisol, leading to an increase in circulating energy resources to restore homeostasis. However, if the allostatic load generated exceeds fish tolerance limits, stress-related responses will compromise health and welfare of the animals. In this context, anesthetics have arisen as potential agents aiming to reduce negative effects of stress response. Here we assessed the effects of a sedative dose of clove oil (CO) and MS-222 on hallmarks involved in HPI axis regulation and energy management after simulated transport, and further recovery, in gilthead seabream (Sparus aurata L.) juveniles. Fish were placed in a mobile setup of water tanks where transport conditions were simulated for 6 h. Sedation doses of either CO (2.5 mg L−1) or MS-222 (5 mg L−1) were added in the water tanks. A control group without anesthetics was also included in the setup. Half of the animals (n = 12 per group) were sampled immediately after transport, while remaining animals were allowed to recover for 18 h in clean water tanks and then sampled. Our results showed that the HPI axis response was modified at peripheral level, with differences depending on the anesthetic employed. Head kidney gene-expressions related to cortisol production (star and cyp11b1) matched concomitantly with increased plasma cortisol levels immediately after transport in CO-sedated fish, but these levels remained constant in MS-222-sedated fish. Differential changes in the energy management of carbohydrates, lipids and amino acids, depending on the anesthetic employed, were also observed. The use of CO stimulated amino acids catabolism, while MS-222-sedated fish tended to consume liver glycogen and mobilize triglycerides. Further studies, including alternative doses of both anestethics, as well as the assessment of time-course HPI activation and longer recovery periods, are necessary to better understand if the use of clove oil and MS-222 is beneficial for S. aurata under these circumstances.This work was funded by Spanish Ministry of Science, Innovation and Universities - MICINN (AGL2013-48835-C2-1-R and AGL2016-76069-C2-1-R) awarded to JM. IJ-C is currently supported by a contract of Trainee Predoctoral Research Staff from the University of Cádiz (PIF UCA/REC02VIT/2014; 2018-011/PU/AY.PUENTE/CD). The authors (IJ-C, IR-J, JM-S, and JM) belong to the Fish Welfare and Stress Network (AGL2016-81808-REDT), supported by the Agencia Estatal de Investigación (MICINN, Spanish Government).Frontiers MediaMinisterio de Economía y Competitividad (España)Universidad de CádizAgencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/209690reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Italiano#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-48835-C2-1-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-76069-C2-1-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-81808-REDThttp://doi.org/10.3389/fphys.2019.00612Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2096902026-05-22T06:33:51Z
score 15,812429

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