Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta
BACKGROUND: Prenatal exposure to endocrine disrupting compounds (EDCs) has previously shown to alter epigenetic marks. OBJECTIVES: In this work we explore whether prenatal exposure to mixtures of xenoestrogens has the potential to alter the placenta epigenome, by studying DNA methylation in retrotra...
| Autores: | , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10230/26908 |
| Acceso en línea: | http://hdl.handle.net/10230/26908 http://dx.doi.org/10.1016/j.envint.2014.06.006 |
| Access Level: | acceso abierto |
| Palabra clave: | Embaràs -- Complicacions Metilació Placenta -- Metabolisme |
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Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placentaVilahur Chiaraviglio, Nadia, 1982-Bustamante Pineda, MarionaByun, Hyang-MinFernandez, Mariana F.Santa Marina, LoretoBasterrechea, MikelBallester Díez, FerranMurcia, MarioTardón, AdoninaFernández Somoano, AnaEstivill, Xavier, 1955-Olea, NicolásSunyer Deu, JordiBaccarelli, Andrea A.Embaràs -- ComplicacionsMetilacióPlacenta -- MetabolismeBACKGROUND: Prenatal exposure to endocrine disrupting compounds (EDCs) has previously shown to alter epigenetic marks. OBJECTIVES: In this work we explore whether prenatal exposure to mixtures of xenoestrogens has the potential to alter the placenta epigenome, by studying DNA methylation in retrotransposons as a surrogate of global DNA methylation. METHODS: The biomarker total effective xenoestrogen burden (TEXB) was measured in 192 placentas from participants in the longitudinal INMA Project. DNA methylation was quantitatively assessed by bisulfite pyrosequencing on 10 different retrotransposons including 3 different long interspersed nuclear elements (LINEs), 4 short interspersed nuclear elements (SINEs) and 3 human endogenous retroviruses (HERVs). Associations were tested using linear mixed-effects regression models and sex interaction was evaluated.RESULTS: A significant sex interaction was observed for AluYb8 (p-value for interaction <0.001, significant at Bonferroni corrected p-value threshold of 0.0025). Boys with the highest TEXB-alpha levels of exposure (third tertile) presented on average a decrease of 0.84% in methylation compared to those in the first tertile (p-value<0.001), while no significant effects were found in girls (p-value=0.134). CONCLUSIONS: Our findings suggest that boys may be more susceptible to the effect of exposure to xenoestrogens during prenatal development, producing shifts in DNA methylation of certain sensitive genomic repetitive sequences in a tissue important for fetal growth and development.This work was supported by grants from the Spanish Ministry of Health (FIS-PI042018; FIS-PI060867; FIS-PI081151; FIS-PI09/02311; FIS-PI09/02647; FIS-PI11/00610); Instituto de Salud Carlos III [Red INMA G03/176 and CB06/02/0041]; the EU Commission (QLK4-1999-01422, QLK4-2002-00603 and CONTAMED FP7-ENV-212502), the Generalitat de Catalunya—CIRIT [1999SGR 00241]; the Consejería de Salud de la Junta de Andalucía (183/07 and 0675/10), the Diputación Foral de Gipuzkoa (DFG06/004), the Department of Health of the Basque Government (2005111093), and by the University of Oviedo, Obra Social Cajastur, the Fundación Roger Torné and La Fundació La Marató de TV3. NV was supported by an FPI Grant from the Spanish Ministry of Health (BES-2009-023933) and a Formación de Personal Investigador Grant for Short Research Stays in Foreign Institutions (BES-2009-023933). AA Baccarelli receives support from the Harvard School of Public Health and National Institute of Environmental Health Sciences Center for Environmental Health (R01 ES021357).The HUSC BioBank, integrated in the Andalusia Public Health System (SSPA) and the National Biobank Network, is financed by the Institute of Health Carlos III, (RD09/0076/00148) and the Regional Government of AndaluciaElsevier201620162014info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/26908http://dx.doi.org/10.1016/j.envint.2014.06.006reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésEnvironment International. 2014 Oct;71:81-7info:eu-repo/grantAgreement/EC/FP7/212502© Elsevier http://dx.doi.org/10.1016/j.envint.2014.06.006info:eu-repo/semantics/openAccessoai:recercat.cat:10230/269082026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta |
| title |
Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta |
| spellingShingle |
Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta Vilahur Chiaraviglio, Nadia, 1982- Embaràs -- Complicacions Metilació Placenta -- Metabolisme |
| title_short |
Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta |
| title_full |
Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta |
| title_fullStr |
Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta |
| title_full_unstemmed |
Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta |
| title_sort |
Prenatal exposure to mixtures of xenoestrogens and repetitive element DNA methylation changes in human placenta |
| dc.creator.none.fl_str_mv |
Vilahur Chiaraviglio, Nadia, 1982- Bustamante Pineda, Mariona Byun, Hyang-Min Fernandez, Mariana F. Santa Marina, Loreto Basterrechea, Mikel Ballester Díez, Ferran Murcia, Mario Tardón, Adonina Fernández Somoano, Ana Estivill, Xavier, 1955- Olea, Nicolás Sunyer Deu, Jordi Baccarelli, Andrea A. |
| author |
Vilahur Chiaraviglio, Nadia, 1982- |
| author_facet |
Vilahur Chiaraviglio, Nadia, 1982- Bustamante Pineda, Mariona Byun, Hyang-Min Fernandez, Mariana F. Santa Marina, Loreto Basterrechea, Mikel Ballester Díez, Ferran Murcia, Mario Tardón, Adonina Fernández Somoano, Ana Estivill, Xavier, 1955- Olea, Nicolás Sunyer Deu, Jordi Baccarelli, Andrea A. |
| author_role |
author |
| author2 |
Bustamante Pineda, Mariona Byun, Hyang-Min Fernandez, Mariana F. Santa Marina, Loreto Basterrechea, Mikel Ballester Díez, Ferran Murcia, Mario Tardón, Adonina Fernández Somoano, Ana Estivill, Xavier, 1955- Olea, Nicolás Sunyer Deu, Jordi Baccarelli, Andrea A. |
| author2_role |
author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Embaràs -- Complicacions Metilació Placenta -- Metabolisme |
| topic |
Embaràs -- Complicacions Metilació Placenta -- Metabolisme |
| description |
BACKGROUND: Prenatal exposure to endocrine disrupting compounds (EDCs) has previously shown to alter epigenetic marks. OBJECTIVES: In this work we explore whether prenatal exposure to mixtures of xenoestrogens has the potential to alter the placenta epigenome, by studying DNA methylation in retrotransposons as a surrogate of global DNA methylation. METHODS: The biomarker total effective xenoestrogen burden (TEXB) was measured in 192 placentas from participants in the longitudinal INMA Project. DNA methylation was quantitatively assessed by bisulfite pyrosequencing on 10 different retrotransposons including 3 different long interspersed nuclear elements (LINEs), 4 short interspersed nuclear elements (SINEs) and 3 human endogenous retroviruses (HERVs). Associations were tested using linear mixed-effects regression models and sex interaction was evaluated.RESULTS: A significant sex interaction was observed for AluYb8 (p-value for interaction <0.001, significant at Bonferroni corrected p-value threshold of 0.0025). Boys with the highest TEXB-alpha levels of exposure (third tertile) presented on average a decrease of 0.84% in methylation compared to those in the first tertile (p-value<0.001), while no significant effects were found in girls (p-value=0.134). CONCLUSIONS: Our findings suggest that boys may be more susceptible to the effect of exposure to xenoestrogens during prenatal development, producing shifts in DNA methylation of certain sensitive genomic repetitive sequences in a tissue important for fetal growth and development. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 2016 2016 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
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article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/26908 http://dx.doi.org/10.1016/j.envint.2014.06.006 |
| url |
http://hdl.handle.net/10230/26908 http://dx.doi.org/10.1016/j.envint.2014.06.006 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Environment International. 2014 Oct;71:81-7 info:eu-repo/grantAgreement/EC/FP7/212502 |
| dc.rights.none.fl_str_mv |
© Elsevier http://dx.doi.org/10.1016/j.envint.2014.06.006 info:eu-repo/semantics/openAccess |
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© Elsevier http://dx.doi.org/10.1016/j.envint.2014.06.006 |
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openAccess |
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application/pdf application/pdf |
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Elsevier |
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Elsevier |
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