Allele-dependent processing pathways generate the endogenous human leukocyte antigen (HLA) class I peptide repertoire in transporters associated with antigen processing (TAP)-deficient cells

The transporters associated with antigen processing (TAP) allow the supply of peptides derived from the cytosol to translocate to the endoplasmic reticulum, where they complex with nascent human leukocyte antigen (HLA) class I molecules. However, infected and tumor cells with TAP molecules blocked o...

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Detalhes bibliográficos
Autores: Lorente, Elena, Garcia, Ruth, Lopez, Daniel
Tipo de documento: artigo
Data de publicação:2011
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositório:Repisalud
Idioma:inglês
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/8583
Acesso em linha:http://hdl.handle.net/20.500.12105/8583
Access Level:Acceso aberto
Palavra-chave:ATP Binding Cassette Transporter, Subfamily B, Member 2
ATP-Binding Cassette Transporters
Alleles
Antigens
CD8-Positive T-Lymphocytes
Cell Line
Cytosol
Endoplasmic Reticulum
HLA Antigens
Histocompatibility Antigens Class I
Humans
Ligands
Peptide Hydrolases
Peptides
Protease Inhibitors
Protein Transport
Surface Properties
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oai_identifier_str oai:repisalud.isciii.es:20.500.12105/8583
network_acronym_str ES
network_name_str España
repository_id_str
spelling Allele-dependent processing pathways generate the endogenous human leukocyte antigen (HLA) class I peptide repertoire in transporters associated with antigen processing (TAP)-deficient cellsLorente, ElenaGarcia, RuthLopez, DanielATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersAllelesAntigensCD8-Positive T-LymphocytesCell LineCytosolEndoplasmic ReticulumHLA AntigensHistocompatibility Antigens Class IHumansLigandsPeptide HydrolasesPeptidesProtease InhibitorsProtein TransportSurface PropertiesThe transporters associated with antigen processing (TAP) allow the supply of peptides derived from the cytosol to translocate to the endoplasmic reticulum, where they complex with nascent human leukocyte antigen (HLA) class I molecules. However, infected and tumor cells with TAP molecules blocked or individuals with nonfunctional TAP complexes are able to present HLA class I ligands generated by TAP-independent processing pathways. These peptides are detected by the CD8(+) lymphocyte cellular response. Here, the generation of the overall peptide repertoire associated with four different HLA class I molecules in TAP-deficient cells was studied. Using different protease inhibitors, four different proteolytic specificities were identified. These data demonstrate the different allele-dependent complex processing pathways involved in the generation of the HLA class I peptide repertoire in TAP-deficient cells.American Society for Biochemistry and Molecular Biology (ASBMB)Fundación para la Investigación y la Prevención del Sida en España20192019-11-1420112011-11-0420112011-11-04journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/8583reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/85832026-06-12T12:43:37Z
dc.title.none.fl_str_mv Allele-dependent processing pathways generate the endogenous human leukocyte antigen (HLA) class I peptide repertoire in transporters associated with antigen processing (TAP)-deficient cells
title Allele-dependent processing pathways generate the endogenous human leukocyte antigen (HLA) class I peptide repertoire in transporters associated with antigen processing (TAP)-deficient cells
spellingShingle Allele-dependent processing pathways generate the endogenous human leukocyte antigen (HLA) class I peptide repertoire in transporters associated with antigen processing (TAP)-deficient cells
Lorente, Elena
ATP Binding Cassette Transporter, Subfamily B, Member 2
ATP-Binding Cassette Transporters
Alleles
Antigens
CD8-Positive T-Lymphocytes
Cell Line
Cytosol
Endoplasmic Reticulum
HLA Antigens
Histocompatibility Antigens Class I
Humans
Ligands
Peptide Hydrolases
Peptides
Protease Inhibitors
Protein Transport
Surface Properties
title_short Allele-dependent processing pathways generate the endogenous human leukocyte antigen (HLA) class I peptide repertoire in transporters associated with antigen processing (TAP)-deficient cells
title_full Allele-dependent processing pathways generate the endogenous human leukocyte antigen (HLA) class I peptide repertoire in transporters associated with antigen processing (TAP)-deficient cells
title_fullStr Allele-dependent processing pathways generate the endogenous human leukocyte antigen (HLA) class I peptide repertoire in transporters associated with antigen processing (TAP)-deficient cells
title_full_unstemmed Allele-dependent processing pathways generate the endogenous human leukocyte antigen (HLA) class I peptide repertoire in transporters associated with antigen processing (TAP)-deficient cells
title_sort Allele-dependent processing pathways generate the endogenous human leukocyte antigen (HLA) class I peptide repertoire in transporters associated with antigen processing (TAP)-deficient cells
dc.creator.none.fl_str_mv Lorente, Elena
Garcia, Ruth
Lopez, Daniel
author Lorente, Elena
author_facet Lorente, Elena
Garcia, Ruth
Lopez, Daniel
author_role author
author2 Garcia, Ruth
Lopez, Daniel
author2_role author
author
dc.contributor.none.fl_str_mv Fundación para la Investigación y la Prevención del Sida en España
dc.subject.none.fl_str_mv ATP Binding Cassette Transporter, Subfamily B, Member 2
ATP-Binding Cassette Transporters
Alleles
Antigens
CD8-Positive T-Lymphocytes
Cell Line
Cytosol
Endoplasmic Reticulum
HLA Antigens
Histocompatibility Antigens Class I
Humans
Ligands
Peptide Hydrolases
Peptides
Protease Inhibitors
Protein Transport
Surface Properties
topic ATP Binding Cassette Transporter, Subfamily B, Member 2
ATP-Binding Cassette Transporters
Alleles
Antigens
CD8-Positive T-Lymphocytes
Cell Line
Cytosol
Endoplasmic Reticulum
HLA Antigens
Histocompatibility Antigens Class I
Humans
Ligands
Peptide Hydrolases
Peptides
Protease Inhibitors
Protein Transport
Surface Properties
description The transporters associated with antigen processing (TAP) allow the supply of peptides derived from the cytosol to translocate to the endoplasmic reticulum, where they complex with nascent human leukocyte antigen (HLA) class I molecules. However, infected and tumor cells with TAP molecules blocked or individuals with nonfunctional TAP complexes are able to present HLA class I ligands generated by TAP-independent processing pathways. These peptides are detected by the CD8(+) lymphocyte cellular response. Here, the generation of the overall peptide repertoire associated with four different HLA class I molecules in TAP-deficient cells was studied. Using different protease inhibitors, four different proteolytic specificities were identified. These data demonstrate the different allele-dependent complex processing pathways involved in the generation of the HLA class I peptide repertoire in TAP-deficient cells.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-11-04
2011
2011-11-04
2019
2019-11-14
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/8583
url http://hdl.handle.net/20.500.12105/8583
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology (ASBMB)
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology (ASBMB)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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