Same-Day Detection of Viable Salmonella Enteritidis by Phage Amplification Coupled with Loop-Mediated Isothermal Amplification

9 pages, 3 tables, 1 figure

Detalles Bibliográficos
Autores: Lamas, Alexandre, Garrido-Maestu, Alejandro
Tipo de recurso: otro
Estado:Versión aceptada para publicación
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/397283
Acceso en línea:http://hdl.handle.net/10261/397283
Access Level:acceso embargado
Palabra clave:Salmonella
Chicken meat
LAMP
Phage
Point of care
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spelling Same-Day Detection of Viable Salmonella Enteritidis by Phage Amplification Coupled with Loop-Mediated Isothermal AmplificationLamas, AlexandreGarrido-Maestu, AlejandroSalmonellaChicken meatLAMPPhagePoint of care9 pages, 3 tables, 1 figureIn recent years, isothermal techniques such as loop-mediated isothermal amplification (LAMP) have appeared as alternatives to qPCR. Due to its amplification at constant temperature, LAMP reactions can be performed in equipment as simple as a thermoblock. However, one of the main limitations of nucleic acid-based techniques is their incapacity to differentiate between live and death cells. To overcome this limitation, some researchers are evaluating the detection of bacteriophages (phages) DNA instead of the detection of bacterial nucleic acids. Phages are used in the sample enrichment step where they reproduce in the presence of live cells. Due to narrow spectrum activity, one phage can infect one bacterial species or even serotype, which allows to perform a selective enrichment. This chapter describes a protocol for the detection of Salmonella Enteritidis in chicken meat in less than 8 h. This method combines sample enrichment in peptone water to which phage PVP-SE2 is added with phage DNA detection by LAMP. In addition, a colorimetric variant of this technique is described that allows this technique to be applied at points of interestPeer reviewedSpringer NatureConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/otherhttp://purl.org/coar/resource_type/c_3248Postprintinfo:eu-repo/semantics/acceptedVersioninfo:eu-repo/semantics/bookParthttp://hdl.handle.net/10261/397283reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésMethods and Protocols in Food Sciencehttps://doi.org/10.1007/978-1-0716-4526-0_18Síinfo:eu-repo/semantics/embargoedAccessoai:digital.csic.es:10261/3972832026-05-22T06:33:51Z
dc.title.none.fl_str_mv Same-Day Detection of Viable Salmonella Enteritidis by Phage Amplification Coupled with Loop-Mediated Isothermal Amplification
title Same-Day Detection of Viable Salmonella Enteritidis by Phage Amplification Coupled with Loop-Mediated Isothermal Amplification
spellingShingle Same-Day Detection of Viable Salmonella Enteritidis by Phage Amplification Coupled with Loop-Mediated Isothermal Amplification
Lamas, Alexandre
Salmonella
Chicken meat
LAMP
Phage
Point of care
title_short Same-Day Detection of Viable Salmonella Enteritidis by Phage Amplification Coupled with Loop-Mediated Isothermal Amplification
title_full Same-Day Detection of Viable Salmonella Enteritidis by Phage Amplification Coupled with Loop-Mediated Isothermal Amplification
title_fullStr Same-Day Detection of Viable Salmonella Enteritidis by Phage Amplification Coupled with Loop-Mediated Isothermal Amplification
title_full_unstemmed Same-Day Detection of Viable Salmonella Enteritidis by Phage Amplification Coupled with Loop-Mediated Isothermal Amplification
title_sort Same-Day Detection of Viable Salmonella Enteritidis by Phage Amplification Coupled with Loop-Mediated Isothermal Amplification
dc.creator.none.fl_str_mv Lamas, Alexandre
Garrido-Maestu, Alejandro
author Lamas, Alexandre
author_facet Lamas, Alexandre
Garrido-Maestu, Alejandro
author_role author
author2 Garrido-Maestu, Alejandro
author2_role author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Salmonella
Chicken meat
LAMP
Phage
Point of care
topic Salmonella
Chicken meat
LAMP
Phage
Point of care
description 9 pages, 3 tables, 1 figure
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/other
http://purl.org/coar/resource_type/c_3248
Postprint
info:eu-repo/semantics/acceptedVersion
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format other
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/397283
url http://hdl.handle.net/10261/397283
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Methods and Protocols in Food Science
https://doi.org/10.1007/978-1-0716-4526-0_18
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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