Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids
We have identified a new human cDNA, L-amino acid transporter-2 (LAT-2), that induces a system L transport activity with 4F2hc (the heavy chain of the surface antigen 4F2, also named CD98) in oocytes. Human LAT-2 is the fourth member of the family of amino acid transporters that are subunits of 4F2h...
| Autores: | , , , , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1999 |
| País: | España |
| Recursos: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/177123 |
| Acesso em linha: | https://hdl.handle.net/2445/177123 |
| Access Level: | acceso abierto |
| Palavra-chave: | Antígens CD Proteïnes portadores Proteïnes de membrana CD antigens Carrier proteins Membrane proteins |
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oai:recercat.cat:2445/177123 |
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Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acidsPineda Riu, MartaFernández, EsperanzaTorrents Arenales, DavidEstévez Povedano, RaúlLópez Iglesias, CarmenCamps Camprubí, MartaLloberas Cavero, JorgeZorzano Olarte, AntonioPalacín Prieto, ManuelAntígens CDProteïnes portadoresProteïnes de membranaCD antigensCarrier proteinsMembrane proteinsWe have identified a new human cDNA, L-amino acid transporter-2 (LAT-2), that induces a system L transport activity with 4F2hc (the heavy chain of the surface antigen 4F2, also named CD98) in oocytes. Human LAT-2 is the fourth member of the family of amino acid transporters that are subunits of 4F2hc. The amino acid transport activity induced by the co-expression of 4F2hc and LAT-2 was sodium-independent and showed broad specificity for small and large zwitterionic amino acids, as well as bulky analogs (e.g. BCH (2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid)). This transport activity was highly trans-stimulated, suggesting an exchanger mechanism of transport. Expression of tagged N-myc-LAT-2 alone in oocytes did not induce amino acid transport, and the protein had an intracellular location. Co-expression of N-myc-LAT-2 and 4F2hc gave amino acid transport induction and expression of N-myc-LAT-2 at the plasma membrane of the oocytes. These data suggest that LAT-2 is an additional member of the family of 4F2 light chain subunits, which associates with 4F2hc to express a system L transport activity with broad specificity for zwitterionic amino acids. Human LAT-2 mRNA is expressed in kidney >>> placenta >> brain, liver > spleen, skeletal muscle, heart, small intestine, and lung. Human LAT-2 gene localizes at chromosome 14q11.2-13 (13 cR or approximately 286 kb from marker D14S1349). The high expression of LAT-2 mRNA in epithelial cells of proximal tubules, the basolateral location of 4F2hc in these cells, and the amino acid transport activity of LAT-2 suggest that this transporter contributes to the renal reabsorption of neutral amino acids in the basolateral domain of epithelial proximal tubule cells.American Society for Biochemistry and Molecular Biology2021202119992021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion7 p.application/pdfhttps://hdl.handle.net/2445/177123Articles publicats en revistes (Ciències Fisiològiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1074/jbc.274.28.19738Journal of Biological Chemistry, 1999, vol. 274, num. 28, p. 19738-19744https://doi.org/10.1074/jbc.274.28.19738(c) American Society for Biochemistry and Molecular Biology, 1999info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1771232026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids |
| title |
Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids |
| spellingShingle |
Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids Pineda Riu, Marta Antígens CD Proteïnes portadores Proteïnes de membrana CD antigens Carrier proteins Membrane proteins |
| title_short |
Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids |
| title_full |
Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids |
| title_fullStr |
Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids |
| title_full_unstemmed |
Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids |
| title_sort |
Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids |
| dc.creator.none.fl_str_mv |
Pineda Riu, Marta Fernández, Esperanza Torrents Arenales, David Estévez Povedano, Raúl López Iglesias, Carmen Camps Camprubí, Marta Lloberas Cavero, Jorge Zorzano Olarte, Antonio Palacín Prieto, Manuel |
| author |
Pineda Riu, Marta |
| author_facet |
Pineda Riu, Marta Fernández, Esperanza Torrents Arenales, David Estévez Povedano, Raúl López Iglesias, Carmen Camps Camprubí, Marta Lloberas Cavero, Jorge Zorzano Olarte, Antonio Palacín Prieto, Manuel |
| author_role |
author |
| author2 |
Fernández, Esperanza Torrents Arenales, David Estévez Povedano, Raúl López Iglesias, Carmen Camps Camprubí, Marta Lloberas Cavero, Jorge Zorzano Olarte, Antonio Palacín Prieto, Manuel |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Antígens CD Proteïnes portadores Proteïnes de membrana CD antigens Carrier proteins Membrane proteins |
| topic |
Antígens CD Proteïnes portadores Proteïnes de membrana CD antigens Carrier proteins Membrane proteins |
| description |
We have identified a new human cDNA, L-amino acid transporter-2 (LAT-2), that induces a system L transport activity with 4F2hc (the heavy chain of the surface antigen 4F2, also named CD98) in oocytes. Human LAT-2 is the fourth member of the family of amino acid transporters that are subunits of 4F2hc. The amino acid transport activity induced by the co-expression of 4F2hc and LAT-2 was sodium-independent and showed broad specificity for small and large zwitterionic amino acids, as well as bulky analogs (e.g. BCH (2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid)). This transport activity was highly trans-stimulated, suggesting an exchanger mechanism of transport. Expression of tagged N-myc-LAT-2 alone in oocytes did not induce amino acid transport, and the protein had an intracellular location. Co-expression of N-myc-LAT-2 and 4F2hc gave amino acid transport induction and expression of N-myc-LAT-2 at the plasma membrane of the oocytes. These data suggest that LAT-2 is an additional member of the family of 4F2 light chain subunits, which associates with 4F2hc to express a system L transport activity with broad specificity for zwitterionic amino acids. Human LAT-2 mRNA is expressed in kidney >>> placenta >> brain, liver > spleen, skeletal muscle, heart, small intestine, and lung. Human LAT-2 gene localizes at chromosome 14q11.2-13 (13 cR or approximately 286 kb from marker D14S1349). The high expression of LAT-2 mRNA in epithelial cells of proximal tubules, the basolateral location of 4F2hc in these cells, and the amino acid transport activity of LAT-2 suggest that this transporter contributes to the renal reabsorption of neutral amino acids in the basolateral domain of epithelial proximal tubule cells. |
| publishDate |
1999 |
| dc.date.none.fl_str_mv |
1999 2021 2021 2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/177123 |
| url |
https://hdl.handle.net/2445/177123 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.1074/jbc.274.28.19738 Journal of Biological Chemistry, 1999, vol. 274, num. 28, p. 19738-19744 https://doi.org/10.1074/jbc.274.28.19738 |
| dc.rights.none.fl_str_mv |
(c) American Society for Biochemistry and Molecular Biology, 1999 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
(c) American Society for Biochemistry and Molecular Biology, 1999 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
7 p. application/pdf |
| dc.publisher.none.fl_str_mv |
American Society for Biochemistry and Molecular Biology |
| publisher.none.fl_str_mv |
American Society for Biochemistry and Molecular Biology |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Ciències Fisiològiques) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str |
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| reponame_str |
Recercat. Dipósit de la Recerca de Catalunya |
| collection |
Recercat. Dipósit de la Recerca de Catalunya |
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1869424461648756736 |
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15.812429 |