A proteomics approach to decipher the molecular nature of planarian stem cells.

Background In recent years, planaria have emerged as an important model system for research into stem cells and regeneration. Attention is focused on their unique stem cells, the neoblasts, which can differentiate into any cell type present in the adult organism. Sequencing of the Schmidtea mediterr...

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Autores: Fernandez-Taboada, Enrique, Rodríguez Esteban, Gustavo, Saló i Boix, Emili, Abril Ferrando, Josep Francesc, 1970-
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/18329
Acesso em linha:https://hdl.handle.net/2445/18329
Access Level:acceso abierto
Palavra-chave:Proteòmica
Embriologia
Regeneració (Biologia)
Cèl·lules mare
Planària
Proteomics
Embryology
Regeneration (Biology)
Stem cells
Planaria
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spelling A proteomics approach to decipher the molecular nature of planarian stem cells.Fernandez-Taboada, EnriqueRodríguez Esteban, GustavoSaló i Boix, EmiliAbril Ferrando, Josep Francesc, 1970-ProteòmicaEmbriologiaEmbriologiaRegeneració (Biologia)Cèl·lules marePlanàriaProteomicsEmbryologyRegeneration (Biology)Stem cellsPlanariaBackground In recent years, planaria have emerged as an important model system for research into stem cells and regeneration. Attention is focused on their unique stem cells, the neoblasts, which can differentiate into any cell type present in the adult organism. Sequencing of the Schmidtea mediterranea genome and some expressed sequence tag projects have generated extensive data on the genetic profile of these cells. However, little information is available on their protein dynamics. Results We developed a proteomic strategy to identify neoblast-specific proteins. Here we describe the method and discuss the results in comparison to the genomic high-throughput analyses carried out in planaria and to proteomic studies using other stem cell systems. We also show functional data for some of the candidate genes selected in our proteomic approach. Conclusions We have developed an accurate and reliable mass-spectra-based proteomics approach to complement previous genomic studies and to further achieve a more accurate understanding and description of the molecular and cellular processes related to the neoblasts.BioMed Central2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/18329Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1186/1471-2164-12-133BMC Genomics, 2011, 12:133http://dx.doi.org/10.1186/1471-2164-12-133cc-by, (c) Fernandez-Taboada et al., 2011http://creativecommons.org/licenses/by/2.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/183292026-05-27T06:46:51Z
dc.title.none.fl_str_mv A proteomics approach to decipher the molecular nature of planarian stem cells.
title A proteomics approach to decipher the molecular nature of planarian stem cells.
spellingShingle A proteomics approach to decipher the molecular nature of planarian stem cells.
Fernandez-Taboada, Enrique
Proteòmica
Embriologia
Embriologia
Regeneració (Biologia)
Cèl·lules mare
Planària
Proteomics
Embryology
Regeneration (Biology)
Stem cells
Planaria
title_short A proteomics approach to decipher the molecular nature of planarian stem cells.
title_full A proteomics approach to decipher the molecular nature of planarian stem cells.
title_fullStr A proteomics approach to decipher the molecular nature of planarian stem cells.
title_full_unstemmed A proteomics approach to decipher the molecular nature of planarian stem cells.
title_sort A proteomics approach to decipher the molecular nature of planarian stem cells.
dc.creator.none.fl_str_mv Fernandez-Taboada, Enrique
Rodríguez Esteban, Gustavo
Saló i Boix, Emili
Abril Ferrando, Josep Francesc, 1970-
author Fernandez-Taboada, Enrique
author_facet Fernandez-Taboada, Enrique
Rodríguez Esteban, Gustavo
Saló i Boix, Emili
Abril Ferrando, Josep Francesc, 1970-
author_role author
author2 Rodríguez Esteban, Gustavo
Saló i Boix, Emili
Abril Ferrando, Josep Francesc, 1970-
author2_role author
author
author
dc.subject.none.fl_str_mv Proteòmica
Embriologia
Embriologia
Regeneració (Biologia)
Cèl·lules mare
Planària
Proteomics
Embryology
Regeneration (Biology)
Stem cells
Planaria
topic Proteòmica
Embriologia
Embriologia
Regeneració (Biologia)
Cèl·lules mare
Planària
Proteomics
Embryology
Regeneration (Biology)
Stem cells
Planaria
description Background In recent years, planaria have emerged as an important model system for research into stem cells and regeneration. Attention is focused on their unique stem cells, the neoblasts, which can differentiate into any cell type present in the adult organism. Sequencing of the Schmidtea mediterranea genome and some expressed sequence tag projects have generated extensive data on the genetic profile of these cells. However, little information is available on their protein dynamics. Results We developed a proteomic strategy to identify neoblast-specific proteins. Here we describe the method and discuss the results in comparison to the genomic high-throughput analyses carried out in planaria and to proteomic studies using other stem cell systems. We also show functional data for some of the candidate genes selected in our proteomic approach. Conclusions We have developed an accurate and reliable mass-spectra-based proteomics approach to complement previous genomic studies and to further achieve a more accurate understanding and description of the molecular and cellular processes related to the neoblasts.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/18329
url https://hdl.handle.net/2445/18329
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1186/1471-2164-12-133
BMC Genomics, 2011, 12:133
http://dx.doi.org/10.1186/1471-2164-12-133
dc.rights.none.fl_str_mv cc-by, (c) Fernandez-Taboada et al., 2011
http://creativecommons.org/licenses/by/2.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by, (c) Fernandez-Taboada et al., 2011
http://creativecommons.org/licenses/by/2.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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