Isolation and characterization of an exopolymer produced by bacillus licheniformis: in vitro antiviral activity against enveloped viruses

The exopolymer (EPSp) produced by the strain B. licheniformis IDN-EC was isolated and characterized using different techniques (MALDI-TOF, NMR, ATR-FTIR, TGA, DSC, SEM). The results showed that the low molecular weight EPSp contained a long polyglutamic acid and an extracellular teichoic acid polysa...

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Autores: Sánchez-León, Enrique, Bello-Morales, Raquel, López-Guerrero, José Antonio, Poveda, A., Jiménez-Barbero, Jesús, Gironès, Núria, Abrusci, C.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/216632
Acceso en línea:http://hdl.handle.net/10261/216632
Access Level:acceso abierto
Palabra clave:Bacillus licheniformis
Poly(γ-glutamic acid)
Teichoic acids
Antiviral
Enveloped viruses
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spelling Isolation and characterization of an exopolymer produced by bacillus licheniformis: in vitro antiviral activity against enveloped virusesSánchez-León, EnriqueBello-Morales, RaquelLópez-Guerrero, José AntonioPoveda, A.Jiménez-Barbero, JesúsGironès, NúriaAbrusci, C.Bacillus licheniformisPoly(γ-glutamic acid)Teichoic acidsAntiviralEnveloped virusesThe exopolymer (EPSp) produced by the strain B. licheniformis IDN-EC was isolated and characterized using different techniques (MALDI-TOF, NMR, ATR-FTIR, TGA, DSC, SEM). The results showed that the low molecular weight EPSp contained a long polyglutamic acid and an extracellular teichoic acid polysaccharide. The latter was composed of poly(glycerol phosphate) and was substituted at the 2-position of the glycerol residues with a αGal and αGlcNH2. The αGal O-6 position was also found to be substituted by a phosphate group. The antiviral capability of this EPSp was also tested on both enveloped (herpesviruses HSV, PRV and vesicular stomatitis VSV) and non-enveloped (MVM) viruses. The EPSp was efficient at inhibiting viral entry for the herpesviruses and VSV but was not effective against non-enveloped viruses. The in vivo assay of the EPSp in mice showed no signs of toxicity which could allow for its application in the healthcare sector.Peer reviewedElsevier BVConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/216632reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.carbpol.2020.116737Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2166322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Isolation and characterization of an exopolymer produced by bacillus licheniformis: in vitro antiviral activity against enveloped viruses
title Isolation and characterization of an exopolymer produced by bacillus licheniformis: in vitro antiviral activity against enveloped viruses
spellingShingle Isolation and characterization of an exopolymer produced by bacillus licheniformis: in vitro antiviral activity against enveloped viruses
Sánchez-León, Enrique
Bacillus licheniformis
Poly(γ-glutamic acid)
Teichoic acids
Antiviral
Enveloped viruses
title_short Isolation and characterization of an exopolymer produced by bacillus licheniformis: in vitro antiviral activity against enveloped viruses
title_full Isolation and characterization of an exopolymer produced by bacillus licheniformis: in vitro antiviral activity against enveloped viruses
title_fullStr Isolation and characterization of an exopolymer produced by bacillus licheniformis: in vitro antiviral activity against enveloped viruses
title_full_unstemmed Isolation and characterization of an exopolymer produced by bacillus licheniformis: in vitro antiviral activity against enveloped viruses
title_sort Isolation and characterization of an exopolymer produced by bacillus licheniformis: in vitro antiviral activity against enveloped viruses
dc.creator.none.fl_str_mv Sánchez-León, Enrique
Bello-Morales, Raquel
López-Guerrero, José Antonio
Poveda, A.
Jiménez-Barbero, Jesús
Gironès, Núria
Abrusci, C.
author Sánchez-León, Enrique
author_facet Sánchez-León, Enrique
Bello-Morales, Raquel
López-Guerrero, José Antonio
Poveda, A.
Jiménez-Barbero, Jesús
Gironès, Núria
Abrusci, C.
author_role author
author2 Bello-Morales, Raquel
López-Guerrero, José Antonio
Poveda, A.
Jiménez-Barbero, Jesús
Gironès, Núria
Abrusci, C.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacillus licheniformis
Poly(γ-glutamic acid)
Teichoic acids
Antiviral
Enveloped viruses
topic Bacillus licheniformis
Poly(γ-glutamic acid)
Teichoic acids
Antiviral
Enveloped viruses
description The exopolymer (EPSp) produced by the strain B. licheniformis IDN-EC was isolated and characterized using different techniques (MALDI-TOF, NMR, ATR-FTIR, TGA, DSC, SEM). The results showed that the low molecular weight EPSp contained a long polyglutamic acid and an extracellular teichoic acid polysaccharide. The latter was composed of poly(glycerol phosphate) and was substituted at the 2-position of the glycerol residues with a αGal and αGlcNH2. The αGal O-6 position was also found to be substituted by a phosphate group. The antiviral capability of this EPSp was also tested on both enveloped (herpesviruses HSV, PRV and vesicular stomatitis VSV) and non-enveloped (MVM) viruses. The EPSp was efficient at inhibiting viral entry for the herpesviruses and VSV but was not effective against non-enveloped viruses. The in vivo assay of the EPSp in mice showed no signs of toxicity which could allow for its application in the healthcare sector.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/216632
url http://hdl.handle.net/10261/216632
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.carbpol.2020.116737
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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