Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in sparus aurata

The administration of a single dose of chitosan nanoparticles driving the expression of sterol regulatory element-binding protein 1a (SREBP1a) was recently associated with the enhanced conversion of carbohydrates into lipids. To address the effects of the long-lasting expression of SREBP1a on the gr...

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Autores: Rashidpour, Ania, Almajano Pablos, María Pilar|||0000-0002-1127-3608, Fàbregas Cores, Anna, Meton Teijeiro, Isidoro, Wu, Yuanbing
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/395976
Acceso en línea:https://hdl.handle.net/2117/395976
https://dx.doi.org/10.3390/md21110562
Access Level:acceso abierto
Palabra clave:Chitosan
Sparus aurata
SREBP1
Gene delivery
Liver
Growth
Quitonsan
Orada
Àrees temàtiques de la UPC::Enginyeria química
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spelling Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in sparus aurataRashidpour, AniaAlmajano Pablos, María Pilar|||0000-0002-1127-3608Fàbregas Cores, AnnaMeton Teijeiro, IsidoroWu, YuanbingChitosanSparus aurataChitosanSREBP1Gene deliveryLiverGrowthSparus aurataQuitonsanOradaÀrees temàtiques de la UPC::Enginyeria químicaThe administration of a single dose of chitosan nanoparticles driving the expression of sterol regulatory element-binding protein 1a (SREBP1a) was recently associated with the enhanced conversion of carbohydrates into lipids. To address the effects of the long-lasting expression of SREBP1a on the growth and liver intermediary metabolism of carnivorous fish, chitosan-tripolyphosphate (TPP) nanoparticles complexed with a plasmid expressing the N terminal active domain of hamster SREBP1a (pSG5-SREBP1a) were injected intraperitoneally every 4 weeks (three doses in total) to gilthead sea bream (Sparus aurata) fed high-protein–low-carbohydrate and low-protein–high-carbohydrate diets. Following 70 days of treatment, chitosan-TPP-pSG5-SREBP1a nanoparticles led to the sustained upregulation of SREBP1a in the liver of S. aurata. Independently of the diet, SREBP1a overexpression significantly increased their weight gain, specific growth rate, and protein efficiency ratio but decreased their feed conversion ratio. In agreement with an improved conversion of dietary carbohydrates into lipids, SREBP1a expression increased serum triglycerides and cholesterol as well as hepatic glucose oxidation via glycolysis and the pentose phosphate pathway, while not affecting gluconeogenesis and transamination. Our findings support that the periodical administration of chitosan-TPP-DNA nanoparticles to overexpress SREBP1a in the liver enhanced the growth performance of S. aurata through a mechanism that enabled protein sparing by enhancing dietary carbohydrate metabolisation.This research was funded by the Ministerio de Ciencia e Innovación (Spain) and cofunded by the European Regional Development Fund (European Comission), grant number PID2021-125642OB-I00.  Peer ReviewedObjectius de Desenvolupament Sostenible::12 - Producció i Consum Responsables20232023-10-2620232023-11-08journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/395976https://dx.doi.org/10.3390/md21110562reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3959762026-05-27T15:37:01Z
dc.title.none.fl_str_mv Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in sparus aurata
title Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in sparus aurata
spellingShingle Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in sparus aurata
Rashidpour, Ania
Chitosan
Sparus aurata
Chitosan
SREBP1
Gene delivery
Liver
Growth
Sparus aurata
Quitonsan
Orada
Àrees temàtiques de la UPC::Enginyeria química
title_short Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in sparus aurata
title_full Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in sparus aurata
title_fullStr Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in sparus aurata
title_full_unstemmed Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in sparus aurata
title_sort Chitosan-based sustained expression of sterol regulatory element-binding protein 1a stimulates hepatic glucose oxidation and growth in sparus aurata
dc.creator.none.fl_str_mv Rashidpour, Ania
Almajano Pablos, María Pilar|||0000-0002-1127-3608
Fàbregas Cores, Anna
Meton Teijeiro, Isidoro
Wu, Yuanbing
author Rashidpour, Ania
author_facet Rashidpour, Ania
Almajano Pablos, María Pilar|||0000-0002-1127-3608
Fàbregas Cores, Anna
Meton Teijeiro, Isidoro
Wu, Yuanbing
author_role author
author2 Almajano Pablos, María Pilar|||0000-0002-1127-3608
Fàbregas Cores, Anna
Meton Teijeiro, Isidoro
Wu, Yuanbing
author2_role author
author
author
author
dc.subject.none.fl_str_mv Chitosan
Sparus aurata
Chitosan
SREBP1
Gene delivery
Liver
Growth
Sparus aurata
Quitonsan
Orada
Àrees temàtiques de la UPC::Enginyeria química
topic Chitosan
Sparus aurata
Chitosan
SREBP1
Gene delivery
Liver
Growth
Sparus aurata
Quitonsan
Orada
Àrees temàtiques de la UPC::Enginyeria química
description The administration of a single dose of chitosan nanoparticles driving the expression of sterol regulatory element-binding protein 1a (SREBP1a) was recently associated with the enhanced conversion of carbohydrates into lipids. To address the effects of the long-lasting expression of SREBP1a on the growth and liver intermediary metabolism of carnivorous fish, chitosan-tripolyphosphate (TPP) nanoparticles complexed with a plasmid expressing the N terminal active domain of hamster SREBP1a (pSG5-SREBP1a) were injected intraperitoneally every 4 weeks (three doses in total) to gilthead sea bream (Sparus aurata) fed high-protein–low-carbohydrate and low-protein–high-carbohydrate diets. Following 70 days of treatment, chitosan-TPP-pSG5-SREBP1a nanoparticles led to the sustained upregulation of SREBP1a in the liver of S. aurata. Independently of the diet, SREBP1a overexpression significantly increased their weight gain, specific growth rate, and protein efficiency ratio but decreased their feed conversion ratio. In agreement with an improved conversion of dietary carbohydrates into lipids, SREBP1a expression increased serum triglycerides and cholesterol as well as hepatic glucose oxidation via glycolysis and the pentose phosphate pathway, while not affecting gluconeogenesis and transamination. Our findings support that the periodical administration of chitosan-TPP-DNA nanoparticles to overexpress SREBP1a in the liver enhanced the growth performance of S. aurata through a mechanism that enabled protein sparing by enhancing dietary carbohydrate metabolisation.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-10-26
2023
2023-11-08
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/395976
https://dx.doi.org/10.3390/md21110562
url https://hdl.handle.net/2117/395976
https://dx.doi.org/10.3390/md21110562
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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