Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight Adults

Time-restricted eating (TRE), a popular form of intermittent fasting, has been demonstrated to provide multiple health benefits, including an extension of healthy lifespan in preclinical models. While the specific mechanisms remain elusive, emerging research indicates that one plausible mechanism th...

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Autores: Saini, Sunil K, Singh, Arashdeep, Saini, Manisha, Gonzalez-Freire, Marta, Leeuwenburgh, Christiaan, Anton, Stephen D.
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/19879
Acceso en línea:https://hdl.handle.net/20.500.13003/19879
Access Level:acceso abierto
Palabra clave:Aged
MicroRNAs
Pilot Projects
Adult
Fasting
Humans
Weight Loss
Overweight
Pérdida de Peso
Proyectos Piloto
Humanos
Ayuno
Anciano
Adulto
Sobrepeso
MicroARNs
intermittent fasting
weight loss
cell survival
diet
fat loss
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oai_identifier_str oai:docusalut.com:20.500.13003/19879
network_acronym_str ES
network_name_str España
repository_id_str
spelling Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight AdultsSaini, Sunil KSingh, ArashdeepSaini, ManishaGonzalez-Freire, MartaLeeuwenburgh, ChristiaanAnton, Stephen D.AgedMicroRNAsPilot ProjectsAdultFastingHumansWeight LossOverweightPérdida de PesoProyectos PilotoHumanosAyunoAncianoAdultoSobrepesoMicroARNsintermittent fastingweight losscell survivaldietfat lossTime-restricted eating (TRE), a popular form of intermittent fasting, has been demonstrated to provide multiple health benefits, including an extension of healthy lifespan in preclinical models. While the specific mechanisms remain elusive, emerging research indicates that one plausible mechanism through which TRE may confer health benefits is by influencing the expression of the epigenetic modulator circulatory miRNAs, which serve as intercellular communicators and are dysregulated in metabolic disorders, such as obesity. Therefore, the goal of this pilot study is to examine the effects of a 4-week TRE regimen on global circulatory miRNA from older (>= 65 years) overweight participants. Pre- and post-TRE regimen serum samples from nine individuals who participated in the Time to Eat clinical trial (NCT03590847) and had a significant weight loss (2.6 kg, p < 0.01) were analyzed. The expressions of 2083 human miRNAs were quantified using HTG molecular whole transcriptome miRNA assay. In silico analyses were performed to determine the target genes and biological pathways associated with differentially expressed miRNAs to predict the metabolic effects of the TRE regimen. Fourteen miRNAs were differentially expressed pre- and post-TRE regimen. Specifically, downregulated miRNA targets suggested increased expression of transcripts, including PTEN, TSC1, and ULK1, and were related to cell growth and survival. Furthermore, the targets of downregulated miRNAs were associated with Ras signaling (cell growth and proliferation), mTOR signaling (cell growth and protein synthesis), insulin signaling (glucose uptake), and autophagy (cellular homeostasis and survival). In conclusion, the TRE regimen downregulated miRNA, which, in turn, could inhibit the pathways of cell growth and activate the pathways of cell survival and might promote healthy aging. Future mechanistic studies are required to understand the functional role of the miRNAs reported in this study.MDPI20222022-05-0120222022-05-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/19879reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/198792026-06-22T12:44:07Z
dc.title.none.fl_str_mv Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight Adults
title Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight Adults
spellingShingle Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight Adults
Saini, Sunil K
Aged
MicroRNAs
Pilot Projects
Adult
Fasting
Humans
Weight Loss
Overweight
Pérdida de Peso
Proyectos Piloto
Humanos
Ayuno
Anciano
Adulto
Sobrepeso
MicroARNs
intermittent fasting
weight loss
cell survival
diet
fat loss
title_short Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight Adults
title_full Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight Adults
title_fullStr Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight Adults
title_full_unstemmed Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight Adults
title_sort Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight Adults
dc.creator.none.fl_str_mv Saini, Sunil K
Singh, Arashdeep
Saini, Manisha
Gonzalez-Freire, Marta
Leeuwenburgh, Christiaan
Anton, Stephen D.
author Saini, Sunil K
author_facet Saini, Sunil K
Singh, Arashdeep
Saini, Manisha
Gonzalez-Freire, Marta
Leeuwenburgh, Christiaan
Anton, Stephen D.
author_role author
author2 Singh, Arashdeep
Saini, Manisha
Gonzalez-Freire, Marta
Leeuwenburgh, Christiaan
Anton, Stephen D.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Aged
MicroRNAs
Pilot Projects
Adult
Fasting
Humans
Weight Loss
Overweight
Pérdida de Peso
Proyectos Piloto
Humanos
Ayuno
Anciano
Adulto
Sobrepeso
MicroARNs
intermittent fasting
weight loss
cell survival
diet
fat loss
topic Aged
MicroRNAs
Pilot Projects
Adult
Fasting
Humans
Weight Loss
Overweight
Pérdida de Peso
Proyectos Piloto
Humanos
Ayuno
Anciano
Adulto
Sobrepeso
MicroARNs
intermittent fasting
weight loss
cell survival
diet
fat loss
description Time-restricted eating (TRE), a popular form of intermittent fasting, has been demonstrated to provide multiple health benefits, including an extension of healthy lifespan in preclinical models. While the specific mechanisms remain elusive, emerging research indicates that one plausible mechanism through which TRE may confer health benefits is by influencing the expression of the epigenetic modulator circulatory miRNAs, which serve as intercellular communicators and are dysregulated in metabolic disorders, such as obesity. Therefore, the goal of this pilot study is to examine the effects of a 4-week TRE regimen on global circulatory miRNA from older (>= 65 years) overweight participants. Pre- and post-TRE regimen serum samples from nine individuals who participated in the Time to Eat clinical trial (NCT03590847) and had a significant weight loss (2.6 kg, p < 0.01) were analyzed. The expressions of 2083 human miRNAs were quantified using HTG molecular whole transcriptome miRNA assay. In silico analyses were performed to determine the target genes and biological pathways associated with differentially expressed miRNAs to predict the metabolic effects of the TRE regimen. Fourteen miRNAs were differentially expressed pre- and post-TRE regimen. Specifically, downregulated miRNA targets suggested increased expression of transcripts, including PTEN, TSC1, and ULK1, and were related to cell growth and survival. Furthermore, the targets of downregulated miRNAs were associated with Ras signaling (cell growth and proliferation), mTOR signaling (cell growth and protein synthesis), insulin signaling (glucose uptake), and autophagy (cellular homeostasis and survival). In conclusion, the TRE regimen downregulated miRNA, which, in turn, could inhibit the pathways of cell growth and activate the pathways of cell survival and might promote healthy aging. Future mechanistic studies are required to understand the functional role of the miRNAs reported in this study.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-05-01
2022
2022-05-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/19879
url https://hdl.handle.net/20.500.13003/19879
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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