A Genetic Dissection of Natural Variation for Stomatal Abundance Traits in Arabidopsis

Stomatal abundance varies widely across natural populations of Arabidopsis thaliana, and presumably affects plant performance because it influences water and CO2 exchange with the atmosphere and thence photosynthesis and transpiration. In order to determine the genetic basis of this natural variatio...

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Autores: Delgado Delgado, María Dolores, Sánchez-Bermejo , Eduardo, de Marcos Serrano, Alberto, Martín-Jiménez, Cristina, Alonso Blanco, Carlos, Fenoll Comes, María Carmen, Mena Marugan, María de la Montaña
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/46498
Acceso en línea:https://hdl.handle.net/10578/46498
https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01392/full
Access Level:acceso abierto
Palabra clave:Arabidopsis thaliana
Natural variation
Quantitative trait locus
Stomatal abundance
Stomatal development
Stomatal lineage
Stomatal satellite lineages
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spelling A Genetic Dissection of Natural Variation for Stomatal Abundance Traits in ArabidopsisDelgado Delgado, María DoloresSánchez-Bermejo , Eduardode Marcos Serrano, AlbertoMartín-Jiménez, CristinaAlonso Blanco, CarlosFenoll Comes, María CarmenMena Marugan, María de la MontañaArabidopsis thalianaNatural variationQuantitative trait locusStomatal abundanceStomatal developmentStomatal lineageStomatal satellite lineagesStomatal abundance varies widely across natural populations of Arabidopsis thaliana, and presumably affects plant performance because it influences water and CO2 exchange with the atmosphere and thence photosynthesis and transpiration. In order to determine the genetic basis of this natural variation, we have analyzed a recombinant inbred line (RIL) population derived from the wild accession Ll-0 and the reference strain Landsberg erecta (Ler), which show low and high stomatal abundance, respectively. Quantitative trait locus (QTL) analyses of stomatal index, stomatal density, and pavement cell density measured in the adaxial cotyledon epidermis, identified five loci. Three of the genomic regions affect all traits and were named MID (Modulator of Cell Index and Density) 1 to 3. MID2 is a large-effect QTL overlapping with ERECTA (ER), the er-1 allele from Lerincreasing all trait values. Additional analyses of natural and induced loss-of-function ermutations in different genetic backgrounds revealed that ER dysfunctions have differential and opposite effects on the stomatal index in adaxial and abaxial cotyledon epidermis and confirmed that ER is the gene underlying MID2. Ll-0 alleles at MID1 and MID3 displayed moderate and positive effects on the various traits. Furthermore, detailed developmental studies tracking primary and satellite stomatal lineages show that MID3-Ll-0 allele promotes the spacing divisions that initiate satellite lineages, while the ER allele limits them. Finally, expression analyses suggest that ER and MID3 modulate satellization through partly different regulatory pathways. Our characterization of MID3 indicates that genetic modulation of satellization contributes to the variation for stomatal abundance in natural populations, and subsequently that this trait might be involved in plant adaptation.Frontiers Media202620262019info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://hdl.handle.net/10578/46498https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01392/fullreponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésinfo:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/464982026-05-27T07:36:41Z
dc.title.none.fl_str_mv A Genetic Dissection of Natural Variation for Stomatal Abundance Traits in Arabidopsis
title A Genetic Dissection of Natural Variation for Stomatal Abundance Traits in Arabidopsis
spellingShingle A Genetic Dissection of Natural Variation for Stomatal Abundance Traits in Arabidopsis
Delgado Delgado, María Dolores
Arabidopsis thaliana
Natural variation
Quantitative trait locus
Stomatal abundance
Stomatal development
Stomatal lineage
Stomatal satellite lineages
title_short A Genetic Dissection of Natural Variation for Stomatal Abundance Traits in Arabidopsis
title_full A Genetic Dissection of Natural Variation for Stomatal Abundance Traits in Arabidopsis
title_fullStr A Genetic Dissection of Natural Variation for Stomatal Abundance Traits in Arabidopsis
title_full_unstemmed A Genetic Dissection of Natural Variation for Stomatal Abundance Traits in Arabidopsis
title_sort A Genetic Dissection of Natural Variation for Stomatal Abundance Traits in Arabidopsis
dc.creator.none.fl_str_mv Delgado Delgado, María Dolores
Sánchez-Bermejo , Eduardo
de Marcos Serrano, Alberto
Martín-Jiménez, Cristina
Alonso Blanco, Carlos
Fenoll Comes, María Carmen
Mena Marugan, María de la Montaña
author Delgado Delgado, María Dolores
author_facet Delgado Delgado, María Dolores
Sánchez-Bermejo , Eduardo
de Marcos Serrano, Alberto
Martín-Jiménez, Cristina
Alonso Blanco, Carlos
Fenoll Comes, María Carmen
Mena Marugan, María de la Montaña
author_role author
author2 Sánchez-Bermejo , Eduardo
de Marcos Serrano, Alberto
Martín-Jiménez, Cristina
Alonso Blanco, Carlos
Fenoll Comes, María Carmen
Mena Marugan, María de la Montaña
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis thaliana
Natural variation
Quantitative trait locus
Stomatal abundance
Stomatal development
Stomatal lineage
Stomatal satellite lineages
topic Arabidopsis thaliana
Natural variation
Quantitative trait locus
Stomatal abundance
Stomatal development
Stomatal lineage
Stomatal satellite lineages
description Stomatal abundance varies widely across natural populations of Arabidopsis thaliana, and presumably affects plant performance because it influences water and CO2 exchange with the atmosphere and thence photosynthesis and transpiration. In order to determine the genetic basis of this natural variation, we have analyzed a recombinant inbred line (RIL) population derived from the wild accession Ll-0 and the reference strain Landsberg erecta (Ler), which show low and high stomatal abundance, respectively. Quantitative trait locus (QTL) analyses of stomatal index, stomatal density, and pavement cell density measured in the adaxial cotyledon epidermis, identified five loci. Three of the genomic regions affect all traits and were named MID (Modulator of Cell Index and Density) 1 to 3. MID2 is a large-effect QTL overlapping with ERECTA (ER), the er-1 allele from Lerincreasing all trait values. Additional analyses of natural and induced loss-of-function ermutations in different genetic backgrounds revealed that ER dysfunctions have differential and opposite effects on the stomatal index in adaxial and abaxial cotyledon epidermis and confirmed that ER is the gene underlying MID2. Ll-0 alleles at MID1 and MID3 displayed moderate and positive effects on the various traits. Furthermore, detailed developmental studies tracking primary and satellite stomatal lineages show that MID3-Ll-0 allele promotes the spacing divisions that initiate satellite lineages, while the ER allele limits them. Finally, expression analyses suggest that ER and MID3 modulate satellization through partly different regulatory pathways. Our characterization of MID3 indicates that genetic modulation of satellization contributes to the variation for stomatal abundance in natural populations, and subsequently that this trait might be involved in plant adaptation.
publishDate 2019
dc.date.none.fl_str_mv 2019
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10578/46498
https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01392/full
url https://hdl.handle.net/10578/46498
https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01392/full
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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