Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1

[Background]: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. [Results]: To gain insight into the metabolic pathways involved in TAG accumulatio...

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Detalles Bibliográficos
Autores: Juárez, Antonio, Villa Torrecilla, Juan Antonio, Lanza, Val F., Lázaro, Beatriz, Cruz, Fernando de la, Alvarez, Héctor M., Moncalián, Gabriel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/164567
Acceso en línea:http://hdl.handle.net/10261/164567
Access Level:acceso abierto
Palabra clave:CRP
Entner-Doudoroff pathway
RNA-seq
Nutrient starvation
Triacylglycerol
Rhodococcus
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spelling Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1Juárez, AntonioVilla Torrecilla, Juan AntonioLanza, Val F.Lázaro, BeatrizCruz, Fernando de laAlvarez, Héctor M.Moncalián, GabrielCRPEntner-Doudoroff pathwayRNA-seqNutrient starvationTriacylglycerolRhodococcus[Background]: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. [Results]: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMP-dependent CRP regulator. [Conclusion]: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.This work was financed by Grants BIO2010-14809 from the Spanish Ministry of Science and Innovation and BFU2014-55534-C2-2-P from the Spanish Ministry of Economy and Competitiveness to GM. H.M. Alvarez is a career investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.Peer ReviewedBioMed CentralConsejo Nacional de Investigaciones Científicas y Técnicas (Argentina)Ministerio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2018201820172018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/164567reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-55534-C2-2-Phttps://doi.org/10.1186/s12934-017-0651-7Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1645672026-05-22T06:33:51Z
dc.title.none.fl_str_mv Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
spellingShingle Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
Juárez, Antonio
CRP
Entner-Doudoroff pathway
RNA-seq
Nutrient starvation
Triacylglycerol
Rhodococcus
title_short Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_full Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_fullStr Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_full_unstemmed Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
title_sort Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
dc.creator.none.fl_str_mv Juárez, Antonio
Villa Torrecilla, Juan Antonio
Lanza, Val F.
Lázaro, Beatriz
Cruz, Fernando de la
Alvarez, Héctor M.
Moncalián, Gabriel
author Juárez, Antonio
author_facet Juárez, Antonio
Villa Torrecilla, Juan Antonio
Lanza, Val F.
Lázaro, Beatriz
Cruz, Fernando de la
Alvarez, Héctor M.
Moncalián, Gabriel
author_role author
author2 Villa Torrecilla, Juan Antonio
Lanza, Val F.
Lázaro, Beatriz
Cruz, Fernando de la
Alvarez, Héctor M.
Moncalián, Gabriel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv CRP
Entner-Doudoroff pathway
RNA-seq
Nutrient starvation
Triacylglycerol
Rhodococcus
topic CRP
Entner-Doudoroff pathway
RNA-seq
Nutrient starvation
Triacylglycerol
Rhodococcus
description [Background]: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. [Results]: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMP-dependent CRP regulator. [Conclusion]: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/164567
url http://hdl.handle.net/10261/164567
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-55534-C2-2-P
https://doi.org/10.1186/s12934-017-0651-7
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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