Electrospun Polyvinyl Alcohol/Sodium Alginate Nanocomposite Dressings Loaded with ZnO and Bioglass: Characterization, Antibacterial Activity, and Cytocompatibility

Chronic wounds pose a great challenge due to their slow healing and susceptibility to infections, hence the need for innovative alternatives to conventional antibiotics, as increasing bacterial resistance limits the efficacy of current treatments. This paper addresses the development of novel electr...

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Authors: Ortiz, J. Andrés, Sepúlveda, Francesca Antonella, Flores, Siomara, Saavedra, Marcela, Sáez-Silva, Suhelen, Jiménez, Thomas, Murgas, Paola, Troncoso, Scarlett, Sanhueza, Camila, Ulloa, María T., Porte Torre, Lorena, Ahumada, Manuel, Corrales, Teresa, Palza, Humberto, Zapata, Paula A.
Format: article
Status:Published version
Publication Date:2025
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/399004
Online Access:http://hdl.handle.net/10261/399004
Access Level:Open access
Keyword:polyvinyl alcohol
sodium alginate
wound dressing
ZnO
bioglass
cytotoxicity
antibacterial activity
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spelling Electrospun Polyvinyl Alcohol/Sodium Alginate Nanocomposite Dressings Loaded with ZnO and Bioglass: Characterization, Antibacterial Activity, and CytocompatibilityOrtiz, J. AndrésSepúlveda, Francesca AntonellaFlores, SiomaraSaavedra, MarcelaSáez-Silva, SuhelenJiménez, ThomasMurgas, PaolaTroncoso, ScarlettSanhueza, CamilaUlloa, María T.Porte Torre, LorenaAhumada, ManuelCorrales, TeresaPalza, HumbertoZapata, Paula A.polyvinyl alcoholsodium alginatewound dressingZnObioglasscytotoxicityantibacterial activityChronic wounds pose a great challenge due to their slow healing and susceptibility to infections, hence the need for innovative alternatives to conventional antibiotics, as increasing bacterial resistance limits the efficacy of current treatments. This paper addresses the development of novel electrospun membranes based on polyvinyl alcohol (PVA) and sodium alginate, incorporating therapeutic ZnO and bioglass (54SiO<sub>2</sub>:40CaO:6P<sub>2</sub>O<sub>5</sub>) nanoparticles. While nanocomposites presented smaller fiber diameters than pure polymers, ternary nanocomposites displayed higher values, e.g., in porous areas, values were in the ca. 80–240 nm range and 0.06–0.60 μm<sup>2</sup>, respectively. The Young’s modulus of the PVA/SA membrane, initially 15.9 ± 2.0 MPa, decreased by 65% with 10 wt.% ZnO NPs, whereas 10 wt.% BG NPs increased it by 100%. The membranes demonstrated efficacy against Gram-positive bacteria, including methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) isolated from a human wound secretion, as well as two ATCC strains: <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i>. A cell viability assay conducted with HaCaT cells demonstrated nearly complete survival following 72 h of membrane exposure. Their combined Gram-positive antibacterial activity and cytocompatibility support their potential application as biofunctional dressings for the management of chronic and hospital-acquired topical infections, while also contributing to the global effort to combat antibiotic resistance.This research was funded by ANID-FONDECYT 11230657, ANID-Basal Center of Interventional Medicine for Precision and Advanced Cellular Therapy, IMPACT, # FB210024, ANIDFONDECYT 1241229, ANID-FONDECYT 1220093, USACH Fondo VIME consolidación 680-2024 and MINECOProject Ref. PID2021-124926NB-I00. The APC was funded by DICYT Universidad de Santiago de Chile.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Universidad de ChileAgencia Nacional de Investigación y Desarrollo (Chile)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/399004reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124926NB-I00https://doi.org/10.3390/polym17162185Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3990042026-05-22T06:33:51Z
dc.title.none.fl_str_mv Electrospun Polyvinyl Alcohol/Sodium Alginate Nanocomposite Dressings Loaded with ZnO and Bioglass: Characterization, Antibacterial Activity, and Cytocompatibility
title Electrospun Polyvinyl Alcohol/Sodium Alginate Nanocomposite Dressings Loaded with ZnO and Bioglass: Characterization, Antibacterial Activity, and Cytocompatibility
spellingShingle Electrospun Polyvinyl Alcohol/Sodium Alginate Nanocomposite Dressings Loaded with ZnO and Bioglass: Characterization, Antibacterial Activity, and Cytocompatibility
Ortiz, J. Andrés
polyvinyl alcohol
sodium alginate
wound dressing
ZnO
bioglass
cytotoxicity
antibacterial activity
title_short Electrospun Polyvinyl Alcohol/Sodium Alginate Nanocomposite Dressings Loaded with ZnO and Bioglass: Characterization, Antibacterial Activity, and Cytocompatibility
title_full Electrospun Polyvinyl Alcohol/Sodium Alginate Nanocomposite Dressings Loaded with ZnO and Bioglass: Characterization, Antibacterial Activity, and Cytocompatibility
title_fullStr Electrospun Polyvinyl Alcohol/Sodium Alginate Nanocomposite Dressings Loaded with ZnO and Bioglass: Characterization, Antibacterial Activity, and Cytocompatibility
title_full_unstemmed Electrospun Polyvinyl Alcohol/Sodium Alginate Nanocomposite Dressings Loaded with ZnO and Bioglass: Characterization, Antibacterial Activity, and Cytocompatibility
title_sort Electrospun Polyvinyl Alcohol/Sodium Alginate Nanocomposite Dressings Loaded with ZnO and Bioglass: Characterization, Antibacterial Activity, and Cytocompatibility
dc.creator.none.fl_str_mv Ortiz, J. Andrés
Sepúlveda, Francesca Antonella
Flores, Siomara
Saavedra, Marcela
Sáez-Silva, Suhelen
Jiménez, Thomas
Murgas, Paola
Troncoso, Scarlett
Sanhueza, Camila
Ulloa, María T.
Porte Torre, Lorena
Ahumada, Manuel
Corrales, Teresa
Palza, Humberto
Zapata, Paula A.
author Ortiz, J. Andrés
author_facet Ortiz, J. Andrés
Sepúlveda, Francesca Antonella
Flores, Siomara
Saavedra, Marcela
Sáez-Silva, Suhelen
Jiménez, Thomas
Murgas, Paola
Troncoso, Scarlett
Sanhueza, Camila
Ulloa, María T.
Porte Torre, Lorena
Ahumada, Manuel
Corrales, Teresa
Palza, Humberto
Zapata, Paula A.
author_role author
author2 Sepúlveda, Francesca Antonella
Flores, Siomara
Saavedra, Marcela
Sáez-Silva, Suhelen
Jiménez, Thomas
Murgas, Paola
Troncoso, Scarlett
Sanhueza, Camila
Ulloa, María T.
Porte Torre, Lorena
Ahumada, Manuel
Corrales, Teresa
Palza, Humberto
Zapata, Paula A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Universidad de Chile
Agencia Nacional de Investigación y Desarrollo (Chile)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv polyvinyl alcohol
sodium alginate
wound dressing
ZnO
bioglass
cytotoxicity
antibacterial activity
topic polyvinyl alcohol
sodium alginate
wound dressing
ZnO
bioglass
cytotoxicity
antibacterial activity
description Chronic wounds pose a great challenge due to their slow healing and susceptibility to infections, hence the need for innovative alternatives to conventional antibiotics, as increasing bacterial resistance limits the efficacy of current treatments. This paper addresses the development of novel electrospun membranes based on polyvinyl alcohol (PVA) and sodium alginate, incorporating therapeutic ZnO and bioglass (54SiO<sub>2</sub>:40CaO:6P<sub>2</sub>O<sub>5</sub>) nanoparticles. While nanocomposites presented smaller fiber diameters than pure polymers, ternary nanocomposites displayed higher values, e.g., in porous areas, values were in the ca. 80–240 nm range and 0.06–0.60 μm<sup>2</sup>, respectively. The Young’s modulus of the PVA/SA membrane, initially 15.9 ± 2.0 MPa, decreased by 65% with 10 wt.% ZnO NPs, whereas 10 wt.% BG NPs increased it by 100%. The membranes demonstrated efficacy against Gram-positive bacteria, including methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) isolated from a human wound secretion, as well as two ATCC strains: <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i>. A cell viability assay conducted with HaCaT cells demonstrated nearly complete survival following 72 h of membrane exposure. Their combined Gram-positive antibacterial activity and cytocompatibility support their potential application as biofunctional dressings for the management of chronic and hospital-acquired topical infections, while also contributing to the global effort to combat antibiotic resistance.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/399004
url http://hdl.handle.net/10261/399004
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124926NB-I00
https://doi.org/10.3390/polym17162185
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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