Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples

Tetrodotoxin (TTX) is a marine toxin responsible for many intoxications around the world. Its presence in some pufferfish species and, as recently reported, in shellfish, poses a serious health concern. Although TTX is not routinely monitored, there is a need for fast, sensitive, reliable, and simpl...

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Autores: Campàs, Mònica, Reverté, Jaume, Tudó, Àngels, Alkassar, Mounira, Diogène, Jorge, Sureda, Francesc X.
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/2950
Acceso en línea:http://hdl.handle.net/20.500.12327/2950
https://doi.org/10.3390/md22040176
Access Level:acceso abierto
Palabra clave:574
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spelling Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish SamplesCampàs, MònicaReverté, JaumeTudó, ÀngelsAlkassar, MouniraDiogène, JorgeSureda, Francesc X.574Tetrodotoxin (TTX) is a marine toxin responsible for many intoxications around the world. Its presence in some pufferfish species and, as recently reported, in shellfish, poses a serious health concern. Although TTX is not routinely monitored, there is a need for fast, sensitive, reliable, and simple methods for its detection and quantification. In this work, we describe the use of an automated patch clamp (APC) system with Neuro-2a cells for the determination of TTX contents in pufferfish samples. The cells showed an IC50 of 6.4 nM for TTX and were not affected by the presence of muscle, skin, liver, and gonad tissues of a Sphoeroides pachygaster specimen (TTX-free) when analysed at 10 mg/mL. The LOD achieved with this technique was 0.05 mg TTX equiv./kg, which is far below the Japanese regulatory limit of 2 mg TTX equiv./kg. The APC system was applied to the analysis of extracts of a Lagocephalus sceleratus specimen, showing TTX contents that followed the trend of gonads > liver > skin > muscle. The APC system, providing an in vitro toxicological approach, offers the advantages of being sensitive, rapid, and reliable for the detection of TTX-like compounds in seafood.This research was funded by the Ministerio de Ciencia e Innovación (MICIN) and the Agencia Estatal de Investigación (AEI) (Spain) through the CELLECTRA (PID2020-112976RB-C21 and PID2020-112976RB-C22) project. J.R. acknowledges IRTA for his Ph.D. grant (CPI0422). A.T. acknowledges Ministerio de Universidades (Spain) for her Margarita Salas postdoc grant (2021URV-MS-24). M.A. acknowledges MICIN and AEI for her Ph.D. grant (PRE2019-088181). Authors also acknowledge the support from the CERCA Program/Generalitat de Catalunya. The article processing fees were funded by MDPI.info:eu-repo/semantics/publishedVersionMDPIProducció AnimalAigües Marines i Continentals202420242024info:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/20.500.12327/2950https://doi.org/10.3390/md22040176reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésMarine DrugsMC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRAMC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.irta.cat:20.500.12327/29502026-06-16T08:51:17Z
dc.title.none.fl_str_mv Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples
title Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples
spellingShingle Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples
Campàs, Mònica
574
title_short Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples
title_full Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples
title_fullStr Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples
title_full_unstemmed Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples
title_sort Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples
dc.creator.none.fl_str_mv Campàs, Mònica
Reverté, Jaume
Tudó, Àngels
Alkassar, Mounira
Diogène, Jorge
Sureda, Francesc X.
author Campàs, Mònica
author_facet Campàs, Mònica
Reverté, Jaume
Tudó, Àngels
Alkassar, Mounira
Diogène, Jorge
Sureda, Francesc X.
author_role author
author2 Reverté, Jaume
Tudó, Àngels
Alkassar, Mounira
Diogène, Jorge
Sureda, Francesc X.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aigües Marines i Continentals
dc.subject.none.fl_str_mv 574
topic 574
description Tetrodotoxin (TTX) is a marine toxin responsible for many intoxications around the world. Its presence in some pufferfish species and, as recently reported, in shellfish, poses a serious health concern. Although TTX is not routinely monitored, there is a need for fast, sensitive, reliable, and simple methods for its detection and quantification. In this work, we describe the use of an automated patch clamp (APC) system with Neuro-2a cells for the determination of TTX contents in pufferfish samples. The cells showed an IC50 of 6.4 nM for TTX and were not affected by the presence of muscle, skin, liver, and gonad tissues of a Sphoeroides pachygaster specimen (TTX-free) when analysed at 10 mg/mL. The LOD achieved with this technique was 0.05 mg TTX equiv./kg, which is far below the Japanese regulatory limit of 2 mg TTX equiv./kg. The APC system was applied to the analysis of extracts of a Lagocephalus sceleratus specimen, showing TTX contents that followed the trend of gonads > liver > skin > muscle. The APC system, providing an in vitro toxicological approach, offers the advantages of being sensitive, rapid, and reliable for the detection of TTX-like compounds in seafood.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/2950
https://doi.org/10.3390/md22040176
url http://hdl.handle.net/20.500.12327/2950
https://doi.org/10.3390/md22040176
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Marine Drugs
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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