Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study

[eng] Androgen receptor is a transcription factor that plays a key role in prostate cancer development and progression. Its N-terminal intrinsically disordered activation domain is an example of ill- structured negatively charged "acid blob" or "negative noodle" described in 1988...

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Detalles Bibliográficos
Autor: Szulc, Elzbieta Maria
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/148840
Acceso en línea:https://hdl.handle.net/2445/148840
http://hdl.handle.net/10803/668406
Access Level:acceso abierto
Palabra clave:Factors de transcripció
Càncer de pròstata
Andrògens
Proteïnes
Transcription factors
Prostate cancer
Androgens
Proteins
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spelling Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case studySzulc, Elzbieta MariaFactors de transcripcióCàncer de pròstataAndrògensProteïnesTranscription factorsProstate cancerAndrogensProteins[eng] Androgen receptor is a transcription factor that plays a key role in prostate cancer development and progression. Its N-terminal intrinsically disordered activation domain is an example of ill- structured negatively charged "acid blob" or "negative noodle" described in 1988 by Paul B. Sigler. In his model the transcription pre-initiation complex formation relies on non-specific interactions between the "negative noodles" of the transcriptional activating proteins and hydroxyl groups of the heptapeptide repeats of the C-terminal domain in the largest subunit of RNA polymerase II. Here, we take advantage of various biophysical methods to understand the AR activation domain characteristics, its ability to undergo liquid-liquid phase separation and the importance of both for protein-protein interactions of the domain. We show relevance of the secondary structure elements in the AD function despite its general "acid blob" character.Universitat de BarcelonaSalvatella i Giralt, XavierUniversitat de Barcelona. Facultat de Farmàcia2020info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/148840http://hdl.handle.net/10803/668406Tesis Doctorals - Facultat - Farmàcia i Ciències de l'Alimentacióreponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglés(c) Szulc, 2020info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1488402026-05-27T06:46:51Z
dc.title.none.fl_str_mv Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study
title Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study
spellingShingle Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study
Szulc, Elzbieta Maria
Factors de transcripció
Càncer de pròstata
Andrògens
Proteïnes
Transcription factors
Prostate cancer
Androgens
Proteins
title_short Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study
title_full Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study
title_fullStr Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study
title_full_unstemmed Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study
title_sort Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study
dc.creator.none.fl_str_mv Szulc, Elzbieta Maria
author Szulc, Elzbieta Maria
author_facet Szulc, Elzbieta Maria
author_role author
dc.contributor.none.fl_str_mv Salvatella i Giralt, Xavier
Universitat de Barcelona. Facultat de Farmàcia
dc.subject.none.fl_str_mv Factors de transcripció
Càncer de pròstata
Andrògens
Proteïnes
Transcription factors
Prostate cancer
Androgens
Proteins
topic Factors de transcripció
Càncer de pròstata
Andrògens
Proteïnes
Transcription factors
Prostate cancer
Androgens
Proteins
description [eng] Androgen receptor is a transcription factor that plays a key role in prostate cancer development and progression. Its N-terminal intrinsically disordered activation domain is an example of ill- structured negatively charged "acid blob" or "negative noodle" described in 1988 by Paul B. Sigler. In his model the transcription pre-initiation complex formation relies on non-specific interactions between the "negative noodles" of the transcriptional activating proteins and hydroxyl groups of the heptapeptide repeats of the C-terminal domain in the largest subunit of RNA polymerase II. Here, we take advantage of various biophysical methods to understand the AR activation domain characteristics, its ability to undergo liquid-liquid phase separation and the importance of both for protein-protein interactions of the domain. We show relevance of the secondary structure elements in the AD function despite its general "acid blob" character.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/148840
http://hdl.handle.net/10803/668406
url https://hdl.handle.net/2445/148840
http://hdl.handle.net/10803/668406
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv (c) Szulc, 2020
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Szulc, 2020
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universitat de Barcelona
publisher.none.fl_str_mv Universitat de Barcelona
dc.source.none.fl_str_mv Tesis Doctorals - Facultat - Farmàcia i Ciències de l'Alimentació
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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