Isolation and characterization of 16 polymorphic microsatellite loci for Frangula alnus (Rhamnaceae)

We report the first 16 polymorphic nuclear microsatellite markers developed for Frangula alnus (Rhamnaceae). Markers were tested on all three subspecies as well as on three local populations, including analyses of both leaf and seed endocarps. A total of 87 alleles were found (mean number of alleles...

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Detalles Bibliográficos
Autores: Rigueiro, Cristina, Arroyo, Juan M., Rodríguez, R., Hampe, Arndt, Jordano, Pedro
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/38390
Acceso en línea:http://hdl.handle.net/10261/38390
Access Level:acceso abierto
Palabra clave:endocarp
Frangula
Rhamnaceae
seed dispersal
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spelling Isolation and characterization of 16 polymorphic microsatellite loci for Frangula alnus (Rhamnaceae)Rigueiro, CristinaArroyo, Juan M.Rodríguez, R.Hampe, ArndtJordano, PedroJordano, PedroendocarpFrangulaRhamnaceaeseed dispersalWe report the first 16 polymorphic nuclear microsatellite markers developed for Frangula alnus (Rhamnaceae). Markers were tested on all three subspecies as well as on three local populations, including analyses of both leaf and seed endocarps. A total of 87 alleles were found (mean number of alleles per locus was 5.44) for 72 individuals genotyped. Observed and expected heterozygosities ranged from 0.097 to 0.792 and from 0.093 to 0.794, respectively. The levels of polymorphism and exclusionary power of the developed markers render them applicable for parentage analyses and measurements of seed dispersal through direct com- parison of endocarps and adult tree genotypes.Peer reviewedBlackwell Publishing201120112009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/38390reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://onlinelibrary.wiley.com/doi/10.1111/j.1755-0998.2009.02527.x/pdfinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/383902026-05-22T06:33:51Z
dc.title.none.fl_str_mv Isolation and characterization of 16 polymorphic microsatellite loci for Frangula alnus (Rhamnaceae)
title Isolation and characterization of 16 polymorphic microsatellite loci for Frangula alnus (Rhamnaceae)
spellingShingle Isolation and characterization of 16 polymorphic microsatellite loci for Frangula alnus (Rhamnaceae)
Rigueiro, Cristina
endocarp
Frangula
Rhamnaceae
seed dispersal
title_short Isolation and characterization of 16 polymorphic microsatellite loci for Frangula alnus (Rhamnaceae)
title_full Isolation and characterization of 16 polymorphic microsatellite loci for Frangula alnus (Rhamnaceae)
title_fullStr Isolation and characterization of 16 polymorphic microsatellite loci for Frangula alnus (Rhamnaceae)
title_full_unstemmed Isolation and characterization of 16 polymorphic microsatellite loci for Frangula alnus (Rhamnaceae)
title_sort Isolation and characterization of 16 polymorphic microsatellite loci for Frangula alnus (Rhamnaceae)
dc.creator.none.fl_str_mv Rigueiro, Cristina
Arroyo, Juan M.
Rodríguez, R.
Hampe, Arndt
Jordano, Pedro
Jordano, Pedro
author Rigueiro, Cristina
author_facet Rigueiro, Cristina
Arroyo, Juan M.
Rodríguez, R.
Hampe, Arndt
Jordano, Pedro
author_role author
author2 Arroyo, Juan M.
Rodríguez, R.
Hampe, Arndt
Jordano, Pedro
author2_role author
author
author
author
dc.subject.none.fl_str_mv endocarp
Frangula
Rhamnaceae
seed dispersal
topic endocarp
Frangula
Rhamnaceae
seed dispersal
description We report the first 16 polymorphic nuclear microsatellite markers developed for Frangula alnus (Rhamnaceae). Markers were tested on all three subspecies as well as on three local populations, including analyses of both leaf and seed endocarps. A total of 87 alleles were found (mean number of alleles per locus was 5.44) for 72 individuals genotyped. Observed and expected heterozygosities ranged from 0.097 to 0.792 and from 0.093 to 0.794, respectively. The levels of polymorphism and exclusionary power of the developed markers render them applicable for parentage analyses and measurements of seed dispersal through direct com- parison of endocarps and adult tree genotypes.
publishDate 2009
dc.date.none.fl_str_mv 2009
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/38390
url http://hdl.handle.net/10261/38390
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://onlinelibrary.wiley.com/doi/10.1111/j.1755-0998.2009.02527.x/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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