Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses

Understanding the responses of insect herbivores to plant chemical defences is pivotal for the management of crops and pests. However, the mechanisms of interaction are not entirely understood. In this study, we compared the whole transcriptome gene expression of the aphid Macrosiphum euphorbiae gro...

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Authors: Planelló, Rosario, Llorente, Lola, Herrero, Óscar, Novo Rodríguez, Marta, Blanco Sánchez, Lidia, Díaz Pendón, Juan Antonio, Fernández Muñoz, Rafael, Ferrero, Victoria, Peña, Eduardo de la
Format: article
Publication Date:2022
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/123848
Online Access:https://hdl.handle.net/20.500.14352/123848
Access Level:Open access
Keyword:595.754
591.15
632.752
635.1/.8
Molecular biology
Plant sciences
Secondary metabolism
Transcription
Transcriptomics
Insectos
Genética
Agricultura
Biología molecular (Biología)
2413 Biología de Insectos (Entomología)
2401.08 Genética Animal
3107 Horticultura
2415 Biología Molecular
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oai_identifier_str oai:docta.ucm.es:20.500.14352/123848
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repository_id_str
spelling Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responsesPlanelló, RosarioLlorente, LolaHerrero, ÓscarNovo Rodríguez, MartaBlanco Sánchez, LidiaDíaz Pendón, Juan AntonioFernández Muñoz, RafaelFerrero, VictoriaPeña, Eduardo de la595.754591.15632.752635.1/.8Molecular biologyPlant sciencesSecondary metabolismTranscriptionTranscriptomicsInsectosGenéticaAgriculturaBiología molecular (Biología)2413 Biología de Insectos (Entomología)2401.08 Genética Animal3107 Horticultura2415 Biología MolecularUnderstanding the responses of insect herbivores to plant chemical defences is pivotal for the management of crops and pests. However, the mechanisms of interaction are not entirely understood. In this study, we compared the whole transcriptome gene expression of the aphid Macrosiphum euphorbiae grown on two different varieties of tomato that differ in their inducible chemical defences. We used two isogenic lines of tomato with a shared genetic background that only differ in the presence of type IV glandular trichomes and their associated acylsucrose excretions. This works also reports a de novo transcriptome of the aphid M. euphorbiae. Subsequently, we identified a unique and distinct gene expression profile for the first time corresponding to aphid´s exposure to type IV glandular trichomes and acylsugars. The analysis of the aphid transcriptome shows that tomato glandular trichomes and their associated secretions are highly efficient in triggering stress-related responses in the aphid, and demonstrating that their role in plant defence goes beyond the physical impediment of herbivore activity. Some of the differentially expressed genes were associated with carbohydrate, lipid and xenobiotic metabolisms, immune system, oxidative stress response and hormone biosynthesis pathways. Also, the observed responses are compatible with a starvation syndrome. The transcriptome analysis puts forward a wide range of genes involved in the synthesis and regulation of detoxification enzymes that reveal important underlying mechanisms in the interaction of the aphid with its host plant and provides a valuable genomic resource for future study of biological processes at the molecular level using this aphid.Springer NatureUniversidad Complutense de Madrid20222022-11-2320222022-11-23journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/123848reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2015-67733-RMICIU Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 RED2018-102407-Topen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1238482026-06-02T12:44:21Z
dc.title.none.fl_str_mv Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses
title Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses
spellingShingle Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses
Planelló, Rosario
595.754
591.15
632.752
635.1/.8
Molecular biology
Plant sciences
Secondary metabolism
Transcription
Transcriptomics
Insectos
Genética
Agricultura
Biología molecular (Biología)
2413 Biología de Insectos (Entomología)
2401.08 Genética Animal
3107 Horticultura
2415 Biología Molecular
title_short Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses
title_full Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses
title_fullStr Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses
title_full_unstemmed Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses
title_sort Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses
dc.creator.none.fl_str_mv Planelló, Rosario
Llorente, Lola
Herrero, Óscar
Novo Rodríguez, Marta
Blanco Sánchez, Lidia
Díaz Pendón, Juan Antonio
Fernández Muñoz, Rafael
Ferrero, Victoria
Peña, Eduardo de la
author Planelló, Rosario
author_facet Planelló, Rosario
Llorente, Lola
Herrero, Óscar
Novo Rodríguez, Marta
Blanco Sánchez, Lidia
Díaz Pendón, Juan Antonio
Fernández Muñoz, Rafael
Ferrero, Victoria
Peña, Eduardo de la
author_role author
author2 Llorente, Lola
Herrero, Óscar
Novo Rodríguez, Marta
Blanco Sánchez, Lidia
Díaz Pendón, Juan Antonio
Fernández Muñoz, Rafael
Ferrero, Victoria
Peña, Eduardo de la
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 595.754
591.15
632.752
635.1/.8
Molecular biology
Plant sciences
Secondary metabolism
Transcription
Transcriptomics
Insectos
Genética
Agricultura
Biología molecular (Biología)
2413 Biología de Insectos (Entomología)
2401.08 Genética Animal
3107 Horticultura
2415 Biología Molecular
topic 595.754
591.15
632.752
635.1/.8
Molecular biology
Plant sciences
Secondary metabolism
Transcription
Transcriptomics
Insectos
Genética
Agricultura
Biología molecular (Biología)
2413 Biología de Insectos (Entomología)
2401.08 Genética Animal
3107 Horticultura
2415 Biología Molecular
description Understanding the responses of insect herbivores to plant chemical defences is pivotal for the management of crops and pests. However, the mechanisms of interaction are not entirely understood. In this study, we compared the whole transcriptome gene expression of the aphid Macrosiphum euphorbiae grown on two different varieties of tomato that differ in their inducible chemical defences. We used two isogenic lines of tomato with a shared genetic background that only differ in the presence of type IV glandular trichomes and their associated acylsucrose excretions. This works also reports a de novo transcriptome of the aphid M. euphorbiae. Subsequently, we identified a unique and distinct gene expression profile for the first time corresponding to aphid´s exposure to type IV glandular trichomes and acylsugars. The analysis of the aphid transcriptome shows that tomato glandular trichomes and their associated secretions are highly efficient in triggering stress-related responses in the aphid, and demonstrating that their role in plant defence goes beyond the physical impediment of herbivore activity. Some of the differentially expressed genes were associated with carbohydrate, lipid and xenobiotic metabolisms, immune system, oxidative stress response and hormone biosynthesis pathways. Also, the observed responses are compatible with a starvation syndrome. The transcriptome analysis puts forward a wide range of genes involved in the synthesis and regulation of detoxification enzymes that reveal important underlying mechanisms in the interaction of the aphid with its host plant and provides a valuable genomic resource for future study of biological processes at the molecular level using this aphid.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-11-23
2022
2022-11-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/123848
url https://hdl.handle.net/20.500.14352/123848
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available AGL2015-67733-R
MICIU Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 RED2018-102407-T
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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