Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres

To create a chronic glaucoma animal model by a single intracameral injection of biodegradable poly lactic-co-glycolic acid (PLGA) microspheres (Ms) co-loaded with dexamethasone and fibronectin (MsDexaFibro). MsDexaFibro were prepared by a water-in-oil-in-water emulsion method including dexamethasone...

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Autores: Aragón Navas, Alba, Rodrigo, Maria Jesus, García Herranz, David, Martínez, Teresa, Subías, Manuel, Mendez, Silvia, Ruberte, Jesús, Pampalona, Judit, Bravo Osuna, Irene, García Feijoo, Julián, Pablo, Luis E, García Martín, Elena Salobrar, Herrero Vanrell, María Del Rocío
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/73112
Acesso em linha:https://hdl.handle.net/20.500.14352/73112
Access Level:acceso abierto
Palavra-chave:615.4
617.7-007.681
Glaucoma animal model
PLGA microspheres
Multiloaded
Intraocular pressure
Neurodegeneration
Medicina
Oftalmología
Farmacia
Tecnología farmaceútica
32 Ciencias Médicas
3201.09 Oftalmología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/73112
network_acronym_str ES
network_name_str España
repository_id_str
spelling Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheresAragón Navas, AlbaRodrigo, Maria JesusGarcía Herranz, DavidMartínez, TeresaSubías, ManuelMendez, SilviaRuberte, JesúsPampalona, JuditBravo Osuna, IreneGarcía Feijoo, JuliánPablo, Luis EGarcía Martín, Elena SalobrarHerrero Vanrell, María Del Rocío615.4617.7-007.681Glaucoma animal modelPLGA microspheresMultiloadedIntraocular pressureNeurodegenerationMedicinaOftalmologíaFarmaciaTecnología farmaceútica32 Ciencias Médicas3201.09 OftalmologíaTo create a chronic glaucoma animal model by a single intracameral injection of biodegradable poly lactic-co-glycolic acid (PLGA) microspheres (Ms) co-loaded with dexamethasone and fibronectin (MsDexaFibro). MsDexaFibro were prepared by a water-in-oil-in-water emulsion method including dexamethasone in the organic phase and fibronectin in the inner aqueous phase. To create the chronic glaucoma model, an interventionist and longitudinal animal study was performed using forty-five Long Evans rats (4-week-old). Rats received a single intracameral injection of MsDexafibro suspension (10%w/v) in the right eye. Ophthalmological parameters such as clinical signs, intraocular pressure (IOP), neuro-retinal functionality by electroretinography (ERG), retinal structural analysis by optical coherence tomography (OCT), and histology were evaluated up to six months. According to the results obtained, the model proposed was able to induce IOP increasing in both eyes over the study, higher in the injected eyes up to 6 weeks (p < 0.05), while preserving the ocular surface. OCT quantified progressive neuro-retinal degeneration (mainly in the retinal nerve fiber layer) in both eyes but higher in the injected eye. Ganglion cell functionality decreased in injected eyes, thus smaller amplitudes in PhNR were detected by ERG. In conclusion, a new chronic glaucoma animal model was created by a single injection of MsDexaFibro very similar to open-angle glaucoma occurring in humans. This model would impact in different fields such as ophthalmology, allowing long period of study of this pathology; pharmacology, evaluating the neuroprotective activity of active compounds; and pharmaceutical technology, allowing the correct evaluation of the efficacy of long-term sustained ocular drug delivery systems.Taylor and FrancisUniversidad Complutense de Madrid20222022-07-2920222022-07-29journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/73112reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/731122026-06-02T12:44:21Z
dc.title.none.fl_str_mv Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres
title Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres
spellingShingle Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres
Aragón Navas, Alba
615.4
617.7-007.681
Glaucoma animal model
PLGA microspheres
Multiloaded
Intraocular pressure
Neurodegeneration
Medicina
Oftalmología
Farmacia
Tecnología farmaceútica
32 Ciencias Médicas
3201.09 Oftalmología
title_short Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres
title_full Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres
title_fullStr Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres
title_full_unstemmed Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres
title_sort Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres
dc.creator.none.fl_str_mv Aragón Navas, Alba
Rodrigo, Maria Jesus
García Herranz, David
Martínez, Teresa
Subías, Manuel
Mendez, Silvia
Ruberte, Jesús
Pampalona, Judit
Bravo Osuna, Irene
García Feijoo, Julián
Pablo, Luis E
García Martín, Elena Salobrar
Herrero Vanrell, María Del Rocío
author Aragón Navas, Alba
author_facet Aragón Navas, Alba
Rodrigo, Maria Jesus
García Herranz, David
Martínez, Teresa
Subías, Manuel
Mendez, Silvia
Ruberte, Jesús
Pampalona, Judit
Bravo Osuna, Irene
García Feijoo, Julián
Pablo, Luis E
García Martín, Elena Salobrar
Herrero Vanrell, María Del Rocío
author_role author
author2 Rodrigo, Maria Jesus
García Herranz, David
Martínez, Teresa
Subías, Manuel
Mendez, Silvia
Ruberte, Jesús
Pampalona, Judit
Bravo Osuna, Irene
García Feijoo, Julián
Pablo, Luis E
García Martín, Elena Salobrar
Herrero Vanrell, María Del Rocío
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 615.4
617.7-007.681
Glaucoma animal model
PLGA microspheres
Multiloaded
Intraocular pressure
Neurodegeneration
Medicina
Oftalmología
Farmacia
Tecnología farmaceútica
32 Ciencias Médicas
3201.09 Oftalmología
topic 615.4
617.7-007.681
Glaucoma animal model
PLGA microspheres
Multiloaded
Intraocular pressure
Neurodegeneration
Medicina
Oftalmología
Farmacia
Tecnología farmaceútica
32 Ciencias Médicas
3201.09 Oftalmología
description To create a chronic glaucoma animal model by a single intracameral injection of biodegradable poly lactic-co-glycolic acid (PLGA) microspheres (Ms) co-loaded with dexamethasone and fibronectin (MsDexaFibro). MsDexaFibro were prepared by a water-in-oil-in-water emulsion method including dexamethasone in the organic phase and fibronectin in the inner aqueous phase. To create the chronic glaucoma model, an interventionist and longitudinal animal study was performed using forty-five Long Evans rats (4-week-old). Rats received a single intracameral injection of MsDexafibro suspension (10%w/v) in the right eye. Ophthalmological parameters such as clinical signs, intraocular pressure (IOP), neuro-retinal functionality by electroretinography (ERG), retinal structural analysis by optical coherence tomography (OCT), and histology were evaluated up to six months. According to the results obtained, the model proposed was able to induce IOP increasing in both eyes over the study, higher in the injected eyes up to 6 weeks (p < 0.05), while preserving the ocular surface. OCT quantified progressive neuro-retinal degeneration (mainly in the retinal nerve fiber layer) in both eyes but higher in the injected eye. Ganglion cell functionality decreased in injected eyes, thus smaller amplitudes in PhNR were detected by ERG. In conclusion, a new chronic glaucoma animal model was created by a single injection of MsDexaFibro very similar to open-angle glaucoma occurring in humans. This model would impact in different fields such as ophthalmology, allowing long period of study of this pathology; pharmacology, evaluating the neuroprotective activity of active compounds; and pharmaceutical technology, allowing the correct evaluation of the efficacy of long-term sustained ocular drug delivery systems.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-07-29
2022
2022-07-29
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/73112
url https://hdl.handle.net/20.500.14352/73112
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424340655669248
score 15,300719

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