Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization

11 pages, 9 figures

Detalhes bibliográficos
Autores: López-Álvarez, Miriam, González, Pio, Serra, Julia, Fraguas, Javier, Valcárcel Barros, Jesús, Vázquez, José Antonio
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/211392
Acesso em linha:http://hdl.handle.net/10261/211392
Access Level:acceso abierto
Palavra-chave:Natural macromolecules
Chondroitin sulfate
Biomaterials
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spelling Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterizationLópez-Álvarez, MiriamGonzález, PioSerra, JuliaFraguas, JavierValcárcel Barros, JesúsVázquez, José AntonioNatural macromoleculesChondroitin sulfateBiomaterials11 pages, 9 figuresIn the present work, the potential of the Prionace glauca jaw as a source of both chondroitin sulfate and bioapatite is explored. The sandwich-type structure in cross section of the jaw based on alternate layers with prevalence in organic tissue or mineralized is shown and these bands respectively confirmed as CS or hydroxyapatite -enriched zones. As result of this, an optimized process in sequential steps for the recovery of both biomaterials and their purification process is proposed, by combining enzymatic proteolysis, chemical precipitation and separation using ultrafiltration membrane for CS production together with controlled thermal treatment for hydroxyapatite obtaining. The purified CS was characterized by Gel Permeation Chromatography, Nuclear Magnetic Resonance and Strong Anion Exchange Chromatography, revealing a polymeric material with a molecular weight of 67 kDa, and prevalent 6S-GalNAc sulfation (68%), followed by 4S-GalNAc (13%), a significant proportion of disulfated disaccharides (12%) and only 7% of non-sulfated units. In the case of the bioapatite a purified biphasic 60:40 porous calcium phosphate of hydroxyapatite: whitlockite/β-TCP was confirmed. Hydroxyapatite as major component (85%) was also obtained for jaws directly subjected to the thermal treatment. This proved the influence of the enzymatic hydrolysis and centrifugation on the composition of the mineral fractionThis research was partially supported by the European Union projects 0245_IBEROS_1_E and 0302_CVMAR_I_1_P, both from Interreg (POCTEP 2015) and BLUEHUMAN EAPA_151/2016 from INTERREG Atlantic Area, European Regional Development Fund. Moreover, regional funds for Competitive Reference Groups (GRC) ED431C 2017_51, Research networks ED431D 2017/13 and Groups of Potential Growth, all of them from Xunta de Galicia (Spain), are also acknowledged. M. López Álvarez and J. Valcárcel thank funding support provided by 0245_IBEROS_1_E from EU Interreg (POCTEP) projectPeer reviewedElsevierEuropean CommissionXunta de GaliciaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/211392reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.ijbiomac.2020.04.010Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2113922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization
title Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization
spellingShingle Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization
López-Álvarez, Miriam
Natural macromolecules
Chondroitin sulfate
Biomaterials
title_short Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization
title_full Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization
title_fullStr Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization
title_full_unstemmed Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization
title_sort Chondroitin sulfate and hydroxyapatite from Prionace glauca shark jaw: Physicochemical and structural characterization
dc.creator.none.fl_str_mv López-Álvarez, Miriam
González, Pio
Serra, Julia
Fraguas, Javier
Valcárcel Barros, Jesús
Vázquez, José Antonio
author López-Álvarez, Miriam
author_facet López-Álvarez, Miriam
González, Pio
Serra, Julia
Fraguas, Javier
Valcárcel Barros, Jesús
Vázquez, José Antonio
author_role author
author2 González, Pio
Serra, Julia
Fraguas, Javier
Valcárcel Barros, Jesús
Vázquez, José Antonio
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Xunta de Galicia
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Natural macromolecules
Chondroitin sulfate
Biomaterials
topic Natural macromolecules
Chondroitin sulfate
Biomaterials
description 11 pages, 9 figures
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/211392
url http://hdl.handle.net/10261/211392
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.ijbiomac.2020.04.010
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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