Heterogeneity and bipotency of astroglial-like cerebellar progenitors along the interneuron and glial lineages

© 2015 the authors. Cerebellar GABAergic interneurons in mouse comprise multiple subsets of morphologically and neurochemically distinct phenotypes located at strategic nodes of cerebellar local circuits. These cells are produced by common progenitors deriving from the ventricular epithelium during...

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Detalles Bibliográficos
Autores: Parmigiani, E., Leto, K., Rolando, C., Figueres-Oñate, Maria, López-Mascaraque, Laura, Buffo, A., Rossi, F.
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/156749
Acceso en línea:http://hdl.handle.net/10261/156749
Access Level:acceso abierto
Palabra clave:prospective white matter
Neurogenesis
multipotent progenitors
Mouse brain
Cerebellum
Bergmann glia
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spelling Heterogeneity and bipotency of astroglial-like cerebellar progenitors along the interneuron and glial lineagesParmigiani, E.Leto, K.Rolando, C.Figueres-Oñate, MariaLópez-Mascaraque, LauraBuffo, A.Rossi, F.prospective white matterNeurogenesismultipotent progenitorsMouse brainCerebellumBergmann glia© 2015 the authors. Cerebellar GABAergic interneurons in mouse comprise multiple subsets of morphologically and neurochemically distinct phenotypes located at strategic nodes of cerebellar local circuits. These cells are produced by common progenitors deriving from the ventricular epithelium during embryogenesis and from the prospective white matter (PWM) during postnatal development. However, it is not clear whether these progenitors are also shared by other cerebellar lineages and whether germinative sites different from the PWM originate inhibitory interneurons. Indeed, the postnatal cerebellum hosts another germinal site along the Purkinje cell layer (PCL), in which Bergmann glia are generated up to first the postnatal weeks, which was proposed to be neurogenic. Both PCL and PWM comprise precursors displaying traits of juvenile astroglia and neural stem cell markers. First, we examine the proliferative and fate potential of these niches, showing that different proliferative dynamics regulate progenitor amplification at these sites. In addition, PCL and PWM differ in the generated progeny. GABAergic interneurons are produced exclusively by PWM astroglial-like progenitors, whereas PCL precursors produce only astrocytes. Finally, through in vitro, ex vivo, and in vivo clonal analyses we provide evidence that the postnatal PWM hosts a bipotent progenitor that gives rise to both interneurons and white matter astrocytes.Peer ReviewedSociety for NeuroscienceConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720152017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/156749reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1567492026-05-22T06:33:51Z
dc.title.none.fl_str_mv Heterogeneity and bipotency of astroglial-like cerebellar progenitors along the interneuron and glial lineages
title Heterogeneity and bipotency of astroglial-like cerebellar progenitors along the interneuron and glial lineages
spellingShingle Heterogeneity and bipotency of astroglial-like cerebellar progenitors along the interneuron and glial lineages
Parmigiani, E.
prospective white matter
Neurogenesis
multipotent progenitors
Mouse brain
Cerebellum
Bergmann glia
title_short Heterogeneity and bipotency of astroglial-like cerebellar progenitors along the interneuron and glial lineages
title_full Heterogeneity and bipotency of astroglial-like cerebellar progenitors along the interneuron and glial lineages
title_fullStr Heterogeneity and bipotency of astroglial-like cerebellar progenitors along the interneuron and glial lineages
title_full_unstemmed Heterogeneity and bipotency of astroglial-like cerebellar progenitors along the interneuron and glial lineages
title_sort Heterogeneity and bipotency of astroglial-like cerebellar progenitors along the interneuron and glial lineages
dc.creator.none.fl_str_mv Parmigiani, E.
Leto, K.
Rolando, C.
Figueres-Oñate, Maria
López-Mascaraque, Laura
Buffo, A.
Rossi, F.
author Parmigiani, E.
author_facet Parmigiani, E.
Leto, K.
Rolando, C.
Figueres-Oñate, Maria
López-Mascaraque, Laura
Buffo, A.
Rossi, F.
author_role author
author2 Leto, K.
Rolando, C.
Figueres-Oñate, Maria
López-Mascaraque, Laura
Buffo, A.
Rossi, F.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv prospective white matter
Neurogenesis
multipotent progenitors
Mouse brain
Cerebellum
Bergmann glia
topic prospective white matter
Neurogenesis
multipotent progenitors
Mouse brain
Cerebellum
Bergmann glia
description © 2015 the authors. Cerebellar GABAergic interneurons in mouse comprise multiple subsets of morphologically and neurochemically distinct phenotypes located at strategic nodes of cerebellar local circuits. These cells are produced by common progenitors deriving from the ventricular epithelium during embryogenesis and from the prospective white matter (PWM) during postnatal development. However, it is not clear whether these progenitors are also shared by other cerebellar lineages and whether germinative sites different from the PWM originate inhibitory interneurons. Indeed, the postnatal cerebellum hosts another germinal site along the Purkinje cell layer (PCL), in which Bergmann glia are generated up to first the postnatal weeks, which was proposed to be neurogenic. Both PCL and PWM comprise precursors displaying traits of juvenile astroglia and neural stem cell markers. First, we examine the proliferative and fate potential of these niches, showing that different proliferative dynamics regulate progenitor amplification at these sites. In addition, PCL and PWM differ in the generated progeny. GABAergic interneurons are produced exclusively by PWM astroglial-like progenitors, whereas PCL precursors produce only astrocytes. Finally, through in vitro, ex vivo, and in vivo clonal analyses we provide evidence that the postnatal PWM hosts a bipotent progenitor that gives rise to both interneurons and white matter astrocytes.
publishDate 2015
dc.date.none.fl_str_mv 2015
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/156749
url http://hdl.handle.net/10261/156749
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424333300957184
score 15.812429

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