Environmental Compatibility of Penicillium rubens Strain 212

Fusarium wilt causes substantial losses in many crops, and Penicillium rubens strain 212 (PO212) is a well-established biological control agent effective against several soil-borne pathogens, including the causal agents of Fusarium wilt. Before its widespread use, it is essential to assess whether a...

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Autores: Guijarro, Belén, Vazquez, Gema, De Cal, Antonieta, Melgarejo, Paloma, Gaju, Núria|||0000-0002-4951-2096, Martínez-Alonso, Maira|||0000-0002-3321-2558, Larena, Inmaculada
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:325782
Acceso en línea:https://ddd.uab.cat/record/325782
https://dx.doi.org/urn:doi:10.3390/jof11120852
Access Level:acceso abierto
Palabra clave:Penicillium rubens
PO212
Biological control agent
Fusarium oxysporum
Environmental risk assessment
Soil fungal community
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spelling Environmental Compatibility of Penicillium rubens Strain 212Impact on Indigenous Soil Fungal Community DynamicsGuijarro, BelénVazquez, GemaDe Cal, AntonietaMelgarejo, PalomaGaju, Núria|||0000-0002-4951-2096Martínez-Alonso, Maira|||0000-0002-3321-2558Larena, InmaculadaPenicillium rubensPO212Biological control agentFusarium oxysporumEnvironmental risk assessmentSoil fungal communityFusarium wilt causes substantial losses in many crops, and Penicillium rubens strain 212 (PO212) is a well-established biological control agent effective against several soil-borne pathogens, including the causal agents of Fusarium wilt. Before its widespread use, it is essential to assess whether applying PO212 may affect indigenous soil microbial communities. To address this, two open-field tomato trials were conducted to evaluate spatial and temporal changes in non-target soil fungal communities following the application of PO212. Fungal community profiles were monitored over one year using PCR-DGGE of fungal rDNA, and representative DGGE bands were sequenced for taxonomic confirmation. Community structure and variability were analysed using cluster analysis (UPGMA and Neighbor-Joining) and analysis of molecular variance (AMOVA) to determine the effects of treatment, sampling date, and soil depth. PO212 application did not significantly altered the composition or diversity of indigenous soil fungal communities. DGGE banding patterns and diversity indices were similar between treated and untreated soils throughout the study period. Observed community changes were driven primarily by temporal (seasonal) variation, with samples collected at 365 days clustering separately from earlier sampling dates for both treatments. AMOVA confirmed that sampling date, rather than PO212 treatment, explained most of the variance in community composition (p < 0.05). Although PO212 persisted in soil, fluctuations in other fungal populations were minor and within the range of natural seasonal variability. Overall, field application of PO212 did not disrupt indigenous soil fungal communities, supporting its environmental safety as a biocontrol agent for managing Fusarium wilt. 22025-01-0120252025-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/325782https://dx.doi.org/urn:doi:10.3390/jof11120852reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Educación y Ciencia RTA2007-00067Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 RTA2010-00093open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3257822026-06-06T12:50:31Z
dc.title.none.fl_str_mv Environmental Compatibility of Penicillium rubens Strain 212
Impact on Indigenous Soil Fungal Community Dynamics
title Environmental Compatibility of Penicillium rubens Strain 212
spellingShingle Environmental Compatibility of Penicillium rubens Strain 212
Guijarro, Belén
Penicillium rubens
PO212
Biological control agent
Fusarium oxysporum
Environmental risk assessment
Soil fungal community
title_short Environmental Compatibility of Penicillium rubens Strain 212
title_full Environmental Compatibility of Penicillium rubens Strain 212
title_fullStr Environmental Compatibility of Penicillium rubens Strain 212
title_full_unstemmed Environmental Compatibility of Penicillium rubens Strain 212
title_sort Environmental Compatibility of Penicillium rubens Strain 212
dc.creator.none.fl_str_mv Guijarro, Belén
Vazquez, Gema
De Cal, Antonieta
Melgarejo, Paloma
Gaju, Núria|||0000-0002-4951-2096
Martínez-Alonso, Maira|||0000-0002-3321-2558
Larena, Inmaculada
author Guijarro, Belén
author_facet Guijarro, Belén
Vazquez, Gema
De Cal, Antonieta
Melgarejo, Paloma
Gaju, Núria|||0000-0002-4951-2096
Martínez-Alonso, Maira|||0000-0002-3321-2558
Larena, Inmaculada
author_role author
author2 Vazquez, Gema
De Cal, Antonieta
Melgarejo, Paloma
Gaju, Núria|||0000-0002-4951-2096
Martínez-Alonso, Maira|||0000-0002-3321-2558
Larena, Inmaculada
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Penicillium rubens
PO212
Biological control agent
Fusarium oxysporum
Environmental risk assessment
Soil fungal community
topic Penicillium rubens
PO212
Biological control agent
Fusarium oxysporum
Environmental risk assessment
Soil fungal community
description Fusarium wilt causes substantial losses in many crops, and Penicillium rubens strain 212 (PO212) is a well-established biological control agent effective against several soil-borne pathogens, including the causal agents of Fusarium wilt. Before its widespread use, it is essential to assess whether applying PO212 may affect indigenous soil microbial communities. To address this, two open-field tomato trials were conducted to evaluate spatial and temporal changes in non-target soil fungal communities following the application of PO212. Fungal community profiles were monitored over one year using PCR-DGGE of fungal rDNA, and representative DGGE bands were sequenced for taxonomic confirmation. Community structure and variability were analysed using cluster analysis (UPGMA and Neighbor-Joining) and analysis of molecular variance (AMOVA) to determine the effects of treatment, sampling date, and soil depth. PO212 application did not significantly altered the composition or diversity of indigenous soil fungal communities. DGGE banding patterns and diversity indices were similar between treated and untreated soils throughout the study period. Observed community changes were driven primarily by temporal (seasonal) variation, with samples collected at 365 days clustering separately from earlier sampling dates for both treatments. AMOVA confirmed that sampling date, rather than PO212 treatment, explained most of the variance in community composition (p < 0.05). Although PO212 persisted in soil, fluctuations in other fungal populations were minor and within the range of natural seasonal variability. Overall, field application of PO212 did not disrupt indigenous soil fungal communities, supporting its environmental safety as a biocontrol agent for managing Fusarium wilt.
publishDate 2025
dc.date.none.fl_str_mv 2
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/325782
https://dx.doi.org/urn:doi:10.3390/jof11120852
url https://ddd.uab.cat/record/325782
https://dx.doi.org/urn:doi:10.3390/jof11120852
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Educación y Ciencia RTA2007-00067
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 RTA2010-00093
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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