Metformin and glucose concentration as limiting factors in retinal pigment epithelial cell viability and proliferation

This article belongs to the Special Issue Cellular and Molecular Mechanisms of Cardiovascular and Metabolic Diseases 2.0.

Bibliographic Details
Authors: Villa-Fernández, Elsa, Garcia, Ana Victoria, Fernández-Fernández, Alejandra, García-Villarino, Miguel, Ares-Blanco, Jessica, Pujante Alarcón, Pedro, González-Vidal, Tomás, Fraga, Mario F., Menéndez-Torre, Edelmiro, Delgado-Álvarez, Elías, Lambert, Carmen
Format: article
Status:Published version
Publication Date:2024
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/349112
Online Access:http://hdl.handle.net/10261/349112
Access Level:Open access
Keyword:Metformin
Glucose
Cell proliferation
Type 2 diabetes
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spelling Metformin and glucose concentration as limiting factors in retinal pigment epithelial cell viability and proliferationVilla-Fernández, ElsaGarcia, Ana VictoriaFernández-Fernández, AlejandraGarcía-Villarino, MiguelAres-Blanco, JessicaPujante Alarcón, PedroGonzález-Vidal, TomásFraga, Mario F.Menéndez-Torre, EdelmiroDelgado-Álvarez, ElíasLambert, CarmenMetforminGlucoseCell proliferationType 2 diabetesThis article belongs to the Special Issue Cellular and Molecular Mechanisms of Cardiovascular and Metabolic Diseases 2.0.Metformin is a well-established drug for the treatment of type 2 diabetes; however, the mechanism of action has not been well described and many aspects of how it truly acts are still unknown. Moreover, regarding in vitro experiments, the glycaemic status when metformin is used is generally not considered, which, added to the suprapharmacological drug concentrations that are commonly employed in research, has resulted in gaps of its mechanism of action. The aim of this study was to determine how glucose and metformin concentrations influence cell culture. Considering that diabetic retinopathy is one of the most common complications of diabetes, a retinal pigment epithelial cell line was selected, and cell viability and proliferation rates were measured at different glucose and metformin concentrations. As expected, glucose concentration by itself positively influenced cell proliferation rates. When the metformin was considered, results were conditioned, as well, by metformin concentration. This conditioning resulted in cell death when high concentrations of metformin were used under physiological concentrations of glucose, while this did not happen when clinically relevant concentrations of metformin were used independently of glucose status. Our study shows the importance of in vitro cell growth conditions when drug effects such as metformin’s are being analysed.This study has been funded by Fundación Caja Rural de Asturias and Sociedad Asturiana de Diabetes, Endocrinología, Nutrición y Obesidad. C.L is a recipient of a Margarita Salas post-doctoral grant from the University of Barcelona.Peer reviewedMultidisciplinary Digital Publishing InstituteCaja Rural de AsturiasSociedad Asturiana de Diabetes, Endocrinología, Nutrición y ObesidadUniversidad de BarcelonaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/349112reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/ijms25052637https://doi.org/10.3390/ijms25052637Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3491122026-05-22T06:33:51Z
dc.title.none.fl_str_mv Metformin and glucose concentration as limiting factors in retinal pigment epithelial cell viability and proliferation
title Metformin and glucose concentration as limiting factors in retinal pigment epithelial cell viability and proliferation
spellingShingle Metformin and glucose concentration as limiting factors in retinal pigment epithelial cell viability and proliferation
Villa-Fernández, Elsa
Metformin
Glucose
Cell proliferation
Type 2 diabetes
title_short Metformin and glucose concentration as limiting factors in retinal pigment epithelial cell viability and proliferation
title_full Metformin and glucose concentration as limiting factors in retinal pigment epithelial cell viability and proliferation
title_fullStr Metformin and glucose concentration as limiting factors in retinal pigment epithelial cell viability and proliferation
title_full_unstemmed Metformin and glucose concentration as limiting factors in retinal pigment epithelial cell viability and proliferation
title_sort Metformin and glucose concentration as limiting factors in retinal pigment epithelial cell viability and proliferation
dc.creator.none.fl_str_mv Villa-Fernández, Elsa
Garcia, Ana Victoria
Fernández-Fernández, Alejandra
García-Villarino, Miguel
Ares-Blanco, Jessica
Pujante Alarcón, Pedro
González-Vidal, Tomás
Fraga, Mario F.
Menéndez-Torre, Edelmiro
Delgado-Álvarez, Elías
Lambert, Carmen
author Villa-Fernández, Elsa
author_facet Villa-Fernández, Elsa
Garcia, Ana Victoria
Fernández-Fernández, Alejandra
García-Villarino, Miguel
Ares-Blanco, Jessica
Pujante Alarcón, Pedro
González-Vidal, Tomás
Fraga, Mario F.
Menéndez-Torre, Edelmiro
Delgado-Álvarez, Elías
Lambert, Carmen
author_role author
author2 Garcia, Ana Victoria
Fernández-Fernández, Alejandra
García-Villarino, Miguel
Ares-Blanco, Jessica
Pujante Alarcón, Pedro
González-Vidal, Tomás
Fraga, Mario F.
Menéndez-Torre, Edelmiro
Delgado-Álvarez, Elías
Lambert, Carmen
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Caja Rural de Asturias
Sociedad Asturiana de Diabetes, Endocrinología, Nutrición y Obesidad
Universidad de Barcelona
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Metformin
Glucose
Cell proliferation
Type 2 diabetes
topic Metformin
Glucose
Cell proliferation
Type 2 diabetes
description This article belongs to the Special Issue Cellular and Molecular Mechanisms of Cardiovascular and Metabolic Diseases 2.0.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/349112
url http://hdl.handle.net/10261/349112
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/ijms25052637
https://doi.org/10.3390/ijms25052637
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.198674

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