Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions

[EN] Bull spermatozoa physiology may be modulated by melatonin. We washed ejaculated spermatozoa free of melatonin and incubated them (4 h, 38 °C) with 0-pM, 1-pM, 100-pM, 10-nM and 1-µM melatonin in TALP-HEPES (non-capacitating) and TALP-HEPES-heparin (capacitating). This range of concentrations en...

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Detalles Bibliográficos
Autores: Fernández Alegre, Estela, Alvarez-Fernandez, Indira, Domínguez Fernández de Tejerina, Juan Carlos, Casao, Adriana, Martínez Pastor, Felipe
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/18004
Acceso en línea:https://www.mdpi.com/1422-0067/21/8/2701
https://hdl.handle.net/10612/18004
Access Level:acceso abierto
Palabra clave:Biología
Melatonin
Spermatozoon
Bull
Sperm motility
Sperm physiology
3104.11 Reproducción
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repository_id_str
spelling Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating ConditionsFernández Alegre, EstelaAlvarez-Fernandez, IndiraDomínguez Fernández de Tejerina, Juan CarlosCasao, AdrianaMartínez Pastor, FelipeBiologíaMelatoninSpermatozoonBullSperm motilitySperm physiology3104.11 Reproducción[EN] Bull spermatozoa physiology may be modulated by melatonin. We washed ejaculated spermatozoa free of melatonin and incubated them (4 h, 38 °C) with 0-pM, 1-pM, 100-pM, 10-nM and 1-µM melatonin in TALP-HEPES (non-capacitating) and TALP-HEPES-heparin (capacitating). This range of concentrations encompassed the effects mediated by melatonin receptors (pM), intracellular targets (nM–µM) or antioxidant activity (µM). Treatment effects were assessed as motility changes by computer-assisted sperm analysis (CASA) of motility and physiological changes by flow cytometry. Melatonin effects were more evident in capacitating conditions, with 100 pM reducing motility and velocity (VCL) while increasing a “slow” subpopulation. All concentrations decreased apoptotic spermatozoa and stimulated mitochondrial activity in viable spermatozoa, with 100 pM–1 µM increasing acrosomal damage, 10 nM–1 µM increasing intracellular calcium and 1 pM reducing the response to a calcium-ionophore challenge. In non-capacitating media, 1 µM increased hyperactivation-related variables and decreased apoptotic spermatozoa; 100 pM–1 µM increased membrane disorders (related to capacitation); all concentrations decreased mitochondrial ROS production. Melatonin concentrations had a modal effect on bull spermatozoa, suggesting a capacitation-modulating role and protective effect at physiological concentrations (pM). Some effects may be of practical use, considering artificial reproductive techniquesSIThis research was funded by MINECO (Spain), Grant Number AGL2013-43328MDPIBiologia CelularFacultad de Ciencias Biologicas y Ambientales2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.mdpi.com/1422-0067/21/8/2701https://hdl.handle.net/10612/18004reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/AGL2013-43328ttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/180042026-06-24T12:43:27Z
dc.title.none.fl_str_mv Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions
title Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions
spellingShingle Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions
Fernández Alegre, Estela
Biología
Melatonin
Spermatozoon
Bull
Sperm motility
Sperm physiology
3104.11 Reproducción
title_short Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions
title_full Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions
title_fullStr Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions
title_full_unstemmed Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions
title_sort Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions
dc.creator.none.fl_str_mv Fernández Alegre, Estela
Alvarez-Fernandez, Indira
Domínguez Fernández de Tejerina, Juan Carlos
Casao, Adriana
Martínez Pastor, Felipe
author Fernández Alegre, Estela
author_facet Fernández Alegre, Estela
Alvarez-Fernandez, Indira
Domínguez Fernández de Tejerina, Juan Carlos
Casao, Adriana
Martínez Pastor, Felipe
author_role author
author2 Alvarez-Fernandez, Indira
Domínguez Fernández de Tejerina, Juan Carlos
Casao, Adriana
Martínez Pastor, Felipe
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Biologia Celular
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Melatonin
Spermatozoon
Bull
Sperm motility
Sperm physiology
3104.11 Reproducción
topic Biología
Melatonin
Spermatozoon
Bull
Sperm motility
Sperm physiology
3104.11 Reproducción
description [EN] Bull spermatozoa physiology may be modulated by melatonin. We washed ejaculated spermatozoa free of melatonin and incubated them (4 h, 38 °C) with 0-pM, 1-pM, 100-pM, 10-nM and 1-µM melatonin in TALP-HEPES (non-capacitating) and TALP-HEPES-heparin (capacitating). This range of concentrations encompassed the effects mediated by melatonin receptors (pM), intracellular targets (nM–µM) or antioxidant activity (µM). Treatment effects were assessed as motility changes by computer-assisted sperm analysis (CASA) of motility and physiological changes by flow cytometry. Melatonin effects were more evident in capacitating conditions, with 100 pM reducing motility and velocity (VCL) while increasing a “slow” subpopulation. All concentrations decreased apoptotic spermatozoa and stimulated mitochondrial activity in viable spermatozoa, with 100 pM–1 µM increasing acrosomal damage, 10 nM–1 µM increasing intracellular calcium and 1 pM reducing the response to a calcium-ionophore challenge. In non-capacitating media, 1 µM increased hyperactivation-related variables and decreased apoptotic spermatozoa; 100 pM–1 µM increased membrane disorders (related to capacitation); all concentrations decreased mitochondrial ROS production. Melatonin concentrations had a modal effect on bull spermatozoa, suggesting a capacitation-modulating role and protective effect at physiological concentrations (pM). Some effects may be of practical use, considering artificial reproductive techniques
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.mdpi.com/1422-0067/21/8/2701
https://hdl.handle.net/10612/18004
url https://www.mdpi.com/1422-0067/21/8/2701
https://hdl.handle.net/10612/18004
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/AGL2013-43328
dc.rights.none.fl_str_mv ttps://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv ttps://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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