Nanopore short-read sequencing: A quick, cost-effective and accurate method for DNA metabarcoding

15 pages, 3 figures, 3 tables, supporting information https://doi.org/10.1002/edn3.374.-- Data Availability Statement: All the data used in this study is publicly available. The raw sequence data is available on NCBI's Sequence Read Archive (http://www.ncbi.nlm.nih.gov/bioproject/898436) with a...

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Detalles Bibliográficos
Autores: Reis, Aimee L. van der, Beckley, Lynnath E., Olivar, M. Pilar, Jeffs, Andrew G.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/304524
Acceso en línea:http://hdl.handle.net/10261/304524
Access Level:acceso abierto
Palabra clave:18S rRNA
COI
Diet analysis
DNA metabarcoding
Hygophum
Illumina
Lanternfish
Nanopore
http://metadata.un.org/sdg/14
Conserve and sustainably use the oceans, seas and marine resources for sustainable development
Descripción
Sumario:15 pages, 3 figures, 3 tables, supporting information https://doi.org/10.1002/edn3.374.-- Data Availability Statement: All the data used in this study is publicly available. The raw sequence data is available on NCBI's Sequence Read Archive (http://www.ncbi.nlm.nih.gov/bioproject/898436) with associated metadata. The raw data provided for Illumina is fastq. The raw data provided for Nanopore is fast5 (a single file in archived format; i.e., pre-guppy) and fastq (post-guppy). All R code and associated data is available on Figshare as an R project (https://doi.org/10.17608/k6.auckland.21482826)