Effects of a mutation in the gyrA gene on the virulence of uropathogenic Escherichia coli

Fluoroquinolones are among the drugs most extensively used for the treatment of bacterial infections in human and veterinary medicine. Resistance to quinolones can be chromosome or plasmid mediated. The chromosomal mechanism of resistance is associated with mutations in the DNA gyrase- and topoisome...

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Autores: Sánchez Céspedes, Javier, Sáez-López, Emma, Frimodt-Mφller, N., Soto González, Sara M., Vila Estapé, Jordi
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/99574
Acceso en línea:https://hdl.handle.net/2445/99574
Access Level:acceso abierto
Palabra clave:Escheríchia coli
Malalties bacterianes
Escherichia coli
Bacterial diseases
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spelling Effects of a mutation in the gyrA gene on the virulence of uropathogenic Escherichia coliSánchez Céspedes, JavierSáez-López, EmmaFrimodt-Mφller, N.Soto González, Sara M.Vila Estapé, JordiEscheríchia coliMalalties bacterianesEscherichia coliBacterial diseasesFluoroquinolones are among the drugs most extensively used for the treatment of bacterial infections in human and veterinary medicine. Resistance to quinolones can be chromosome or plasmid mediated. The chromosomal mechanism of resistance is associated with mutations in the DNA gyrase- and topoisomerase IV-encoding genes and mutations in regulatory genes affecting different efflux systems, among others. We studied the role of the acquisition of a mutation in the gyrA gene in the virulence and protein expression of uropathogenic Escherichia coli (UPEC). The HC14366M strain carrying a mutation in the gyrA gene (S83L) was found to lose the capacity to cause cystitis and pyelonephritis mainly due to a decrease in the expression of the fimA, papA, papB, and ompA genes. The levels of expression of the fimA, papB, and ompA genes were recovered on complementing the strain with a plasmid containing the gyrA wild-type gene. However, only a slight recovery was observed in the colonization of the bladder in the GyrA complement strain compared to the mutant strain in a murine model of ascending urinary tract infection. In conclusion, a mutation in the gyrA gene of uropathogenic E. coli reduced the virulence of the bacteria, likely in association with the effect of DNA supercoiling on the expression of several virulence factors and proteins, thereby decreasing their capacity to cause cystitis and pyelonephritis.American Society for Microbiology2016201620152016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion7 p.application/pdfhttps://hdl.handle.net/2445/99574Articles publicats en revistes (Fonaments Clínics)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: http://dx.doi.org/10.1128/AAC.00665-15Antimicrobial Agents and Chemotherapy, 2015, vol. 59, num. 8, p. 4662-4668http://dx.doi.org/10.1128/AAC.00665-15(c) American Society for Microbiology, 2015info:eu-repo/semantics/openAccessoai:recercat.cat:2445/995742026-05-29T05:05:01Z
dc.title.none.fl_str_mv Effects of a mutation in the gyrA gene on the virulence of uropathogenic Escherichia coli
title Effects of a mutation in the gyrA gene on the virulence of uropathogenic Escherichia coli
spellingShingle Effects of a mutation in the gyrA gene on the virulence of uropathogenic Escherichia coli
Sánchez Céspedes, Javier
Escheríchia coli
Malalties bacterianes
Escherichia coli
Bacterial diseases
title_short Effects of a mutation in the gyrA gene on the virulence of uropathogenic Escherichia coli
title_full Effects of a mutation in the gyrA gene on the virulence of uropathogenic Escherichia coli
title_fullStr Effects of a mutation in the gyrA gene on the virulence of uropathogenic Escherichia coli
title_full_unstemmed Effects of a mutation in the gyrA gene on the virulence of uropathogenic Escherichia coli
title_sort Effects of a mutation in the gyrA gene on the virulence of uropathogenic Escherichia coli
dc.creator.none.fl_str_mv Sánchez Céspedes, Javier
Sáez-López, Emma
Frimodt-Mφller, N.
Soto González, Sara M.
Vila Estapé, Jordi
author Sánchez Céspedes, Javier
author_facet Sánchez Céspedes, Javier
Sáez-López, Emma
Frimodt-Mφller, N.
Soto González, Sara M.
Vila Estapé, Jordi
author_role author
author2 Sáez-López, Emma
Frimodt-Mφller, N.
Soto González, Sara M.
Vila Estapé, Jordi
author2_role author
author
author
author
dc.subject.none.fl_str_mv Escheríchia coli
Malalties bacterianes
Escherichia coli
Bacterial diseases
topic Escheríchia coli
Malalties bacterianes
Escherichia coli
Bacterial diseases
description Fluoroquinolones are among the drugs most extensively used for the treatment of bacterial infections in human and veterinary medicine. Resistance to quinolones can be chromosome or plasmid mediated. The chromosomal mechanism of resistance is associated with mutations in the DNA gyrase- and topoisomerase IV-encoding genes and mutations in regulatory genes affecting different efflux systems, among others. We studied the role of the acquisition of a mutation in the gyrA gene in the virulence and protein expression of uropathogenic Escherichia coli (UPEC). The HC14366M strain carrying a mutation in the gyrA gene (S83L) was found to lose the capacity to cause cystitis and pyelonephritis mainly due to a decrease in the expression of the fimA, papA, papB, and ompA genes. The levels of expression of the fimA, papB, and ompA genes were recovered on complementing the strain with a plasmid containing the gyrA wild-type gene. However, only a slight recovery was observed in the colonization of the bladder in the GyrA complement strain compared to the mutant strain in a murine model of ascending urinary tract infection. In conclusion, a mutation in the gyrA gene of uropathogenic E. coli reduced the virulence of the bacteria, likely in association with the effect of DNA supercoiling on the expression of several virulence factors and proteins, thereby decreasing their capacity to cause cystitis and pyelonephritis.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/99574
url https://hdl.handle.net/2445/99574
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1128/AAC.00665-15
Antimicrobial Agents and Chemotherapy, 2015, vol. 59, num. 8, p. 4662-4668
http://dx.doi.org/10.1128/AAC.00665-15
dc.rights.none.fl_str_mv (c) American Society for Microbiology, 2015
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society for Microbiology, 2015
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7 p.
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv Articles publicats en revistes (Fonaments Clínics)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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