Diagnostic performance of an in-clinic cortisol assay in dogs with suspected hypoadrenocorticism

Objective: To assess the diagnostic accuracy of the rapid point-of-care ELISA by IDEXX (SNAP) for detecting hypoadrenocorticism (HA) in dogs. A secondary aim was to evaluate the agreement between the SNAP and the veterinary reference laboratory (VRL) using chemiluminescent enzyme immunoassay (IMMULI...

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Detalles Bibliográficos
Autores: Torrano Guillamón, Ana, Juárez Sarrión, Casandra, Prosper Asensi, Eva, Castro López, Jorge, García San José, Paula, Arenas Bermejo, Carolina
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/127315
Acceso en línea:https://hdl.handle.net/20.500.14352/127315
Access Level:acceso abierto
Palabra clave:636.09
ELISA
SNAP test
Canine hypoadrenocorticism
Cortisol immunoassay
Hypoadrenocorticism
Veterinaria
3109 Ciencias Veterinarias
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spelling Diagnostic performance of an in-clinic cortisol assay in dogs with suspected hypoadrenocorticismTorrano Guillamón, AnaJuárez Sarrión, CasandraProsper Asensi, EvaCastro López, JorgeGarcía San José, PaulaArenas Bermejo, Carolina636.09ELISASNAP testCanine hypoadrenocorticismCortisol immunoassayHypoadrenocorticismVeterinaria3109 Ciencias VeterinariasObjective: To assess the diagnostic accuracy of the rapid point-of-care ELISA by IDEXX (SNAP) for detecting hypoadrenocorticism (HA) in dogs. A secondary aim was to evaluate the agreement between the SNAP and the veterinary reference laboratory (VRL) using chemiluminescent enzyme immunoassay (IMMULITE 2000) cortisol measurements. We hypothesized that the SNAP would correlate well with the VRL and aid in HA diagnosis. Methods: A prospective observational diagnostic accuracy study was conducted from January 2023 through January 2024, enrolling dogs with suspected HA. Hypoadrenocorticism was diagnosed using an ACTH stimulation test, with basal serum cortisol (BSC) used for screening in some cases. Samples were analyzed by the SNAP and VRL, with the VRL serving as the gold standard. Results: 96 dogs were enrolled; 21 were diagnosed with HA. The SNAP showed correlation with the VRL (Spearman ρ = 0.923; P < .001). Median BSC and post-ACTH cortisol concentrations did not differ significantly between methods. A BSC < 2 µg/dL yielded 100% sensitivity for both, with the SNAP showing higher specificity (73.1%) than the VRL (64.2%). Post-ACTH cortisol < 2 µg/dL was 100% specific by both methods, with 95.2% sensitivity using the SNAP. A 10.5% clinical discordance was observed. Passing-Bablok regression revealed proportional and constant bias, with the SNAP test tending to overestimate cortisol concentrations, but in the low cortisol range this overestimation appears minimal. Conclusions: The SNAP correlated with the VRL for HA diagnosis; however, borderline post-ACTH SNAP results should be confirmed with the VRL. The SNAP-derived BSC may be more specific than the VRL for ruling out HA. Clinical relevance: The SNAP test aids HA diagnosis in urgent care.American Veterinary Medical AssociationUniversidad Complutense de Madrid20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/127315reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1273152026-06-02T12:44:21Z
dc.title.none.fl_str_mv Diagnostic performance of an in-clinic cortisol assay in dogs with suspected hypoadrenocorticism
title Diagnostic performance of an in-clinic cortisol assay in dogs with suspected hypoadrenocorticism
spellingShingle Diagnostic performance of an in-clinic cortisol assay in dogs with suspected hypoadrenocorticism
Torrano Guillamón, Ana
636.09
ELISA
SNAP test
Canine hypoadrenocorticism
Cortisol immunoassay
Hypoadrenocorticism
Veterinaria
3109 Ciencias Veterinarias
title_short Diagnostic performance of an in-clinic cortisol assay in dogs with suspected hypoadrenocorticism
title_full Diagnostic performance of an in-clinic cortisol assay in dogs with suspected hypoadrenocorticism
title_fullStr Diagnostic performance of an in-clinic cortisol assay in dogs with suspected hypoadrenocorticism
title_full_unstemmed Diagnostic performance of an in-clinic cortisol assay in dogs with suspected hypoadrenocorticism
title_sort Diagnostic performance of an in-clinic cortisol assay in dogs with suspected hypoadrenocorticism
dc.creator.none.fl_str_mv Torrano Guillamón, Ana
Juárez Sarrión, Casandra
Prosper Asensi, Eva
Castro López, Jorge
García San José, Paula
Arenas Bermejo, Carolina
author Torrano Guillamón, Ana
author_facet Torrano Guillamón, Ana
Juárez Sarrión, Casandra
Prosper Asensi, Eva
Castro López, Jorge
García San José, Paula
Arenas Bermejo, Carolina
author_role author
author2 Juárez Sarrión, Casandra
Prosper Asensi, Eva
Castro López, Jorge
García San José, Paula
Arenas Bermejo, Carolina
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.09
ELISA
SNAP test
Canine hypoadrenocorticism
Cortisol immunoassay
Hypoadrenocorticism
Veterinaria
3109 Ciencias Veterinarias
topic 636.09
ELISA
SNAP test
Canine hypoadrenocorticism
Cortisol immunoassay
Hypoadrenocorticism
Veterinaria
3109 Ciencias Veterinarias
description Objective: To assess the diagnostic accuracy of the rapid point-of-care ELISA by IDEXX (SNAP) for detecting hypoadrenocorticism (HA) in dogs. A secondary aim was to evaluate the agreement between the SNAP and the veterinary reference laboratory (VRL) using chemiluminescent enzyme immunoassay (IMMULITE 2000) cortisol measurements. We hypothesized that the SNAP would correlate well with the VRL and aid in HA diagnosis. Methods: A prospective observational diagnostic accuracy study was conducted from January 2023 through January 2024, enrolling dogs with suspected HA. Hypoadrenocorticism was diagnosed using an ACTH stimulation test, with basal serum cortisol (BSC) used for screening in some cases. Samples were analyzed by the SNAP and VRL, with the VRL serving as the gold standard. Results: 96 dogs were enrolled; 21 were diagnosed with HA. The SNAP showed correlation with the VRL (Spearman ρ = 0.923; P < .001). Median BSC and post-ACTH cortisol concentrations did not differ significantly between methods. A BSC < 2 µg/dL yielded 100% sensitivity for both, with the SNAP showing higher specificity (73.1%) than the VRL (64.2%). Post-ACTH cortisol < 2 µg/dL was 100% specific by both methods, with 95.2% sensitivity using the SNAP. A 10.5% clinical discordance was observed. Passing-Bablok regression revealed proportional and constant bias, with the SNAP test tending to overestimate cortisol concentrations, but in the low cortisol range this overestimation appears minimal. Conclusions: The SNAP correlated with the VRL for HA diagnosis; however, borderline post-ACTH SNAP results should be confirmed with the VRL. The SNAP-derived BSC may be more specific than the VRL for ruling out HA. Clinical relevance: The SNAP test aids HA diagnosis in urgent care.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/127315
url https://hdl.handle.net/20.500.14352/127315
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Veterinary Medical Association
publisher.none.fl_str_mv American Veterinary Medical Association
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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