An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations
Many herpetofauna species have been introduced outside of their native range. MtDNA barcoding is regularly used to determine the provenance of such populations. The alpine newt has been introduced across the Netherlands, the United Kingdom and Ireland. However, geographical mtDNA structure across th...
| Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/335682 |
| Acceso en línea: | http://hdl.handle.net/10261/335682 |
| Access Level: | acceso abierto |
| Palabra clave: | Citizen science Ichthyosaura alpestris Invasive species MtDNA barcoding Skin swabbing |
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An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populationsRobbemont, Jodyvan Veldhuijzen, SamAllain, Steven J.R.Ambu, JohannaBoyle, RyanCanestrelli, DanieleÓ Cathasaigh, ÉinneCathrine, ChrisChiocchio, AndreaCogalniceanu, DanCvijanovic, MilenaDufresnes, ChristopheEnnis, CollieGandola, RobJablonski, DanielJulian, AngelaKranželic, DariaLukanov, SimeonMartínez-Solano, ÍñigoMontgomery, RyanNaumov, BorislavO'Neill, MatthewNorth, AlexandraPabijan, MaciejPushendorf, RobertSalvi, DanieleSchmidt, BrunoSotiropoulos, KonstantinosStanescu, FlorinaStankovic, DavidStapleton, SarahŠunje, EminaSzabolcs, MártonVacheva, EmiliyaWillis, DavidZimic, AdnanFrance, JamesMeilink, Willem R.M.Stark, TariqStruijk, Richard P.J.H.Theodoropoulos, Anagnostisde Visser, Manon C.Wielstra, BenCitizen scienceIchthyosaura alpestrisInvasive speciesMtDNA barcodingSkin swabbingMany herpetofauna species have been introduced outside of their native range. MtDNA barcoding is regularly used to determine the provenance of such populations. The alpine newt has been introduced across the Netherlands, the United Kingdom and Ireland. However, geographical mtDNA structure across the natural range of the alpine newt is still incompletely understood and certain regions are severely undersampled. We collect mtDNA sequence data of over seven hundred individuals, from both the native and the introduced range. The main new insights from our extended mtDNA phylogeography are that 1) haplotypes from Spain do not form a reciprocally monophyletic clade, but are nested inside the mtDNA clade that covers western and eastern Europe; and 2) haplotypes from the northwest Balkans form a monophyletic clade together with those from the Southern Carpathians and Apuseni Mountains. We also home in on the regions where the distinct mtDNA clades meet in nature. We show that four out of the seven distinct mtDNA clades that comprise the alpine newt are implicated in the introductions in the Netherlands, United Kingdom and Ireland. In several introduced localities, two distinct mtDNA clades co-occur. As these mtDNA clades presumably represent cryptic species, we urge that the extent of genetic admixture between them is assessed from genome-wide nuclear DNA markers. We mobilized a large number of citizen scientists in this project to support the collection of DNA samples by skin swabbing and underscore the effectiveness of this sampling technique for mtDNA barcoding.DS was partially supported by the Slovenian Research Agency (P1-0255). MS was funded by the National Research, Development and Innovation Office of Hungary (NKFIH-OTKA K134391). MON & AT were supported by an Erasmus+ internship.Peer reviewedBrillSlovenian Research AgencyNational Research, Development and Innovation Office (Hungary)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/335682reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://brill.com/view/journals/amre/44/3/article-p347_8.xml?ebody=pdf-67975Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3356822026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations |
| title |
An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations |
| spellingShingle |
An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations Robbemont, Jody Citizen science Ichthyosaura alpestris Invasive species MtDNA barcoding Skin swabbing |
| title_short |
An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations |
| title_full |
An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations |
| title_fullStr |
An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations |
| title_full_unstemmed |
An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations |
| title_sort |
An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations |
| dc.creator.none.fl_str_mv |
Robbemont, Jody van Veldhuijzen, Sam Allain, Steven J.R. Ambu, Johanna Boyle, Ryan Canestrelli, Daniele Ó Cathasaigh, Éinne Cathrine, Chris Chiocchio, Andrea Cogalniceanu, Dan Cvijanovic, Milena Dufresnes, Christophe Ennis, Collie Gandola, Rob Jablonski, Daniel Julian, Angela Kranželic, Daria Lukanov, Simeon Martínez-Solano, Íñigo Montgomery, Ryan Naumov, Borislav O'Neill, Matthew North, Alexandra Pabijan, Maciej Pushendorf, Robert Salvi, Daniele Schmidt, Bruno Sotiropoulos, Konstantinos Stanescu, Florina Stankovic, David Stapleton, Sarah Šunje, Emina Szabolcs, Márton Vacheva, Emiliya Willis, David Zimic, Adnan France, James Meilink, Willem R.M. Stark, Tariq Struijk, Richard P.J.H. Theodoropoulos, Anagnostis de Visser, Manon C. Wielstra, Ben |
| author |
Robbemont, Jody |
| author_facet |
Robbemont, Jody van Veldhuijzen, Sam Allain, Steven J.R. Ambu, Johanna Boyle, Ryan Canestrelli, Daniele Ó Cathasaigh, Éinne Cathrine, Chris Chiocchio, Andrea Cogalniceanu, Dan Cvijanovic, Milena Dufresnes, Christophe Ennis, Collie Gandola, Rob Jablonski, Daniel Julian, Angela Kranželic, Daria Lukanov, Simeon Martínez-Solano, Íñigo Montgomery, Ryan Naumov, Borislav O'Neill, Matthew North, Alexandra Pabijan, Maciej Pushendorf, Robert Salvi, Daniele Schmidt, Bruno Sotiropoulos, Konstantinos Stanescu, Florina Stankovic, David Stapleton, Sarah Šunje, Emina Szabolcs, Márton Vacheva, Emiliya Willis, David Zimic, Adnan France, James Meilink, Willem R.M. Stark, Tariq Struijk, Richard P.J.H. Theodoropoulos, Anagnostis de Visser, Manon C. Wielstra, Ben |
| author_role |
author |
| author2 |
van Veldhuijzen, Sam Allain, Steven J.R. Ambu, Johanna Boyle, Ryan Canestrelli, Daniele Ó Cathasaigh, Éinne Cathrine, Chris Chiocchio, Andrea Cogalniceanu, Dan Cvijanovic, Milena Dufresnes, Christophe Ennis, Collie Gandola, Rob Jablonski, Daniel Julian, Angela Kranželic, Daria Lukanov, Simeon Martínez-Solano, Íñigo Montgomery, Ryan Naumov, Borislav O'Neill, Matthew North, Alexandra Pabijan, Maciej Pushendorf, Robert Salvi, Daniele Schmidt, Bruno Sotiropoulos, Konstantinos Stanescu, Florina Stankovic, David Stapleton, Sarah Šunje, Emina Szabolcs, Márton Vacheva, Emiliya Willis, David Zimic, Adnan France, James Meilink, Willem R.M. Stark, Tariq Struijk, Richard P.J.H. Theodoropoulos, Anagnostis de Visser, Manon C. Wielstra, Ben |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Slovenian Research Agency National Research, Development and Innovation Office (Hungary) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Citizen science Ichthyosaura alpestris Invasive species MtDNA barcoding Skin swabbing |
| topic |
Citizen science Ichthyosaura alpestris Invasive species MtDNA barcoding Skin swabbing |
| description |
Many herpetofauna species have been introduced outside of their native range. MtDNA barcoding is regularly used to determine the provenance of such populations. The alpine newt has been introduced across the Netherlands, the United Kingdom and Ireland. However, geographical mtDNA structure across the natural range of the alpine newt is still incompletely understood and certain regions are severely undersampled. We collect mtDNA sequence data of over seven hundred individuals, from both the native and the introduced range. The main new insights from our extended mtDNA phylogeography are that 1) haplotypes from Spain do not form a reciprocally monophyletic clade, but are nested inside the mtDNA clade that covers western and eastern Europe; and 2) haplotypes from the northwest Balkans form a monophyletic clade together with those from the Southern Carpathians and Apuseni Mountains. We also home in on the regions where the distinct mtDNA clades meet in nature. We show that four out of the seven distinct mtDNA clades that comprise the alpine newt are implicated in the introductions in the Netherlands, United Kingdom and Ireland. In several introduced localities, two distinct mtDNA clades co-occur. As these mtDNA clades presumably represent cryptic species, we urge that the extent of genetic admixture between them is assessed from genome-wide nuclear DNA markers. We mobilized a large number of citizen scientists in this project to support the collection of DNA samples by skin swabbing and underscore the effectiveness of this sampling technique for mtDNA barcoding. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/335682 |
| url |
http://hdl.handle.net/10261/335682 |
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Inglés |
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Inglés |
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https://brill.com/view/journals/amre/44/3/article-p347_8.xml?ebody=pdf-67975 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Brill |
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Brill |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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