Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents

Laveriano-Santos, Emily P.; Marhuenda-Muñoz, María; Vallverdú i Queralt, Anna; Martínez Huélamo, Miriam; Tresserra i Rimbau, Anna; Miliarakis, Eleftherios; Arancibia Riveros, Camila; Jáuregui Pallarés, Olga; Ruíz León, Ana María; Castro-Barquero, Sara; Estruch, Ramon; Bodega, Patricia; Miguel, Mercedes de; Cos Gandoy, Amaya de; Martínez Gomez, Jesús; Santos-Beneit, Gloria; Fernández Alvira, Juan Miguel; Fernández Jiménez, Rodrigo; Lamuela Raventós, Rosa Ma.

Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents

This study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relation...

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Autores:	Laveriano-Santos, Emily P., Marhuenda-Muñoz, María, Vallverdú i Queralt, Anna, Martínez Huélamo, Miriam, Tresserra i Rimbau, Anna, Miliarakis, Eleftherios, Arancibia Riveros, Camila, Jáuregui Pallarés, Olga, Ruíz León, Ana María, Castro-Barquero, Sara, Estruch, Ramon, Bodega, Patricia, Miguel, Mercedes de, Cos Gandoy, Amaya de, Martínez Gomez, Jesús, Santos-Beneit, Gloria, Fernández Alvira, Juan Miguel, Fernández Jiménez, Rodrigo, Lamuela Raventós, Rosa Ma.
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2022
País:	España
Institución:	Universidad de Barcelona
Repositorio:	Dipòsit Digital de la UB
OAI Identifier:	oai:diposit.ub.edu:2445/189942
Acceso en línea:	https://hdl.handle.net/2445/189942
Access Level:	acceso abierto
Palabra clave:	Microbiota intestinal Polifenols Adolescents Gastrointestinal microbiome Polyphenols Teenagers

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spelling	Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in AdolescentsLaveriano-Santos, Emily P.Marhuenda-Muñoz, MaríaVallverdú i Queralt, AnnaMartínez Huélamo, MiriamTresserra i Rimbau, AnnaMiliarakis, EleftheriosArancibia Riveros, CamilaJáuregui Pallarés, OlgaRuíz León, Ana MaríaCastro-Barquero, SaraEstruch, RamonBodega, PatriciaMiguel, Mercedes deCos Gandoy, Amaya deMartínez Gomez, JesúsSantos-Beneit, GloriaFernández Alvira, Juan MiguelFernández Jiménez, RodrigoLamuela Raventós, Rosa Ma.Microbiota intestinalPolifenolsAdolescentsGastrointestinal microbiomePolyphenolsTeenagersThis study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relationship between MPM and dietary (poly)phenols in Spanish adolescents. A total of 601 spot urine samples of adolescents aged 12.02 ± 0.41 years were analyzed. The quantitative method was validated for linearity, limit of detection, limit of quantification, recovery, intra-and inter-day accuracy and precision, as well as postpreparative stability according to the criteria established by the Association of Official Agricultural Chemists International. A total of 17 aglycones and 37 phase II MPM were identified and quantified in 601 spot urine samples. Phenolic acids were the most abundant urinary MPM, whereas stilbenes, hydroxytyrosol, and enterodiol were the least abundant. Urinary hydroxycoumarin acids (urolithins) were positively correlated with flavonoid and total (poly)phenol intake. An HPLC-ESI-LTQ-Orbitrap-HRMS method was developed and fully validated to quantify MPM. The new method was performed accurately and is suitable for MPM quantification in large epidemiological studies. Urinary lignans and urolithins are proposed as potential biomarkers of grain and nut intake in an adolescent population.MDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/189942Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/antiox11061167Antioxidants, 2022, vol. 11, num. 6, p. 1167https://doi.org/10.3390/antiox11061167cc-by (c) Laveriano-Santos, Emily P. et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1899422026-05-27T06:46:51Z
dc.title.none.fl_str_mv	Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents
title	Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents
spellingShingle	Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents Laveriano-Santos, Emily P. Microbiota intestinal Polifenols Adolescents Gastrointestinal microbiome Polyphenols Teenagers
title_short	Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents
title_full	Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents
title_fullStr	Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents
title_full_unstemmed	Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents
title_sort	Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents
dc.creator.none.fl_str_mv	Laveriano-Santos, Emily P. Marhuenda-Muñoz, María Vallverdú i Queralt, Anna Martínez Huélamo, Miriam Tresserra i Rimbau, Anna Miliarakis, Eleftherios Arancibia Riveros, Camila Jáuregui Pallarés, Olga Ruíz León, Ana María Castro-Barquero, Sara Estruch, Ramon Bodega, Patricia Miguel, Mercedes de Cos Gandoy, Amaya de Martínez Gomez, Jesús Santos-Beneit, Gloria Fernández Alvira, Juan Miguel Fernández Jiménez, Rodrigo Lamuela Raventós, Rosa Ma.
author	Laveriano-Santos, Emily P.
author_facet	Laveriano-Santos, Emily P. Marhuenda-Muñoz, María Vallverdú i Queralt, Anna Martínez Huélamo, Miriam Tresserra i Rimbau, Anna Miliarakis, Eleftherios Arancibia Riveros, Camila Jáuregui Pallarés, Olga Ruíz León, Ana María Castro-Barquero, Sara Estruch, Ramon Bodega, Patricia Miguel, Mercedes de Cos Gandoy, Amaya de Martínez Gomez, Jesús Santos-Beneit, Gloria Fernández Alvira, Juan Miguel Fernández Jiménez, Rodrigo Lamuela Raventós, Rosa Ma.
author_role	author
author2	Marhuenda-Muñoz, María Vallverdú i Queralt, Anna Martínez Huélamo, Miriam Tresserra i Rimbau, Anna Miliarakis, Eleftherios Arancibia Riveros, Camila Jáuregui Pallarés, Olga Ruíz León, Ana María Castro-Barquero, Sara Estruch, Ramon Bodega, Patricia Miguel, Mercedes de Cos Gandoy, Amaya de Martínez Gomez, Jesús Santos-Beneit, Gloria Fernández Alvira, Juan Miguel Fernández Jiménez, Rodrigo Lamuela Raventós, Rosa Ma.
author2_role	author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Microbiota intestinal Polifenols Adolescents Gastrointestinal microbiome Polyphenols Teenagers
topic	Microbiota intestinal Polifenols Adolescents Gastrointestinal microbiome Polyphenols Teenagers
description	This study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relationship between MPM and dietary (poly)phenols in Spanish adolescents. A total of 601 spot urine samples of adolescents aged 12.02 ± 0.41 years were analyzed. The quantitative method was validated for linearity, limit of detection, limit of quantification, recovery, intra-and inter-day accuracy and precision, as well as postpreparative stability according to the criteria established by the Association of Official Agricultural Chemists International. A total of 17 aglycones and 37 phase II MPM were identified and quantified in 601 spot urine samples. Phenolic acids were the most abundant urinary MPM, whereas stilbenes, hydroxytyrosol, and enterodiol were the least abundant. Urinary hydroxycoumarin acids (urolithins) were positively correlated with flavonoid and total (poly)phenol intake. An HPLC-ESI-LTQ-Orbitrap-HRMS method was developed and fully validated to quantify MPM. The new method was performed accurately and is suitable for MPM quantification in large epidemiological studies. Urinary lignans and urolithins are proposed as potential biomarkers of grain and nut intake in an adolescent population.
publishDate	2022
dc.date.none.fl_str_mv	2022
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/189942
url	https://hdl.handle.net/2445/189942
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.3390/antiox11061167 Antioxidants, 2022, vol. 11, num. 6, p. 1167 https://doi.org/10.3390/antiox11061167
dc.rights.none.fl_str_mv	cc-by (c) Laveriano-Santos, Emily P. et al., 2022 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc-by (c) Laveriano-Santos, Emily P. et al., 2022 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona
instname_str	Universidad de Barcelona
reponame_str	Dipòsit Digital de la UB
collection	Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,300719

Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents

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